Amphiphilic polymers formed from ring-opening polymerization: a strategy for the enhancement of gene delivery

2017 ◽  
Vol 5 (4) ◽  
pp. 718-729 ◽  
Author(s):  
Yi-Mei Zhang ◽  
Zheng Huang ◽  
Ji Zhang ◽  
Wan-Xia Wu ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Ring Opening Polymerization ◽  
Amphiphilic Polymers ◽  
Cationic Lipids ◽  
Promising Strategy

Ring-opening polymerization was found to be a promising strategy to improve the transfection efficiency and serum tolerance of cationic lipids.

Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles

2015 ◽  
Vol 3 (13) ◽  
pp. 2660-2670 ◽  
Author(s):  
Qian Guo ◽  
Yan-Hong Liu ◽  
Miao-Miao Xun ◽  
Ji Zhang ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Glycidyl Ether ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

PEI 600-based polymers were synthesized via ring-opening polymerization and exhibited much better transfection efficiency and biocompatibility than PEI 25 kDa.

Cationic polymer-derived carbon dots for enhanced gene delivery and cell imaging

2019 ◽  
Vol 7 (5) ◽  
pp. 1940-1948 ◽  
Author(s):  
Xi He ◽  
Ping Chen ◽  
Ji Zhang ◽  
Tian-Ying Luo ◽  
Hai-Jiao Wang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Carbon Dots ◽  
Ring Opening ◽  
Cell Imaging ◽  
High Efficiency ◽  
Ring Opening Polymerization ◽  
Cationic Polymer ◽  
Cationic Polymers ◽  
Promising Strategy ◽  
Gene Vectors

The transformation of cationic polymers derived from ring-opening polymerization to carbon dots was proved as a promising strategy for developing gene vectors with high efficiency and cell-imaging ability.

Bio-reducible polycations from ring-opening polymerization as potential gene delivery vehicles

2016 ◽  
Vol 14 (27) ◽  
pp. 6470-6478 ◽  
Author(s):  
Qing-Ying Yu ◽  
Yan-Hong Liu ◽  
Zheng Huang ◽  
Ji Zhang ◽  
Chao-Ran Luan ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Ring Opening Polymerization ◽  
Dna Condensation ◽  
Molecular Weights ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

Bio-reducible polycations were prepared via ring-opening polymerization. These materials have relatively low molecular weights and cytotoxicity but have good DNA condensation ability, transfection efficiency and excellent serum tolerance.

Stepwise Development of Biomimetic Chimeric Peptides for Gene Delivery

2020 ◽  
Vol 27 (8) ◽  
pp. 698-710
Author(s):  
Roya Cheraghi ◽  
Mahboobeh Nazari ◽  
Mohsen Alipour ◽  
Saman Hosseinkhani
Keyword(s):  
Gene Delivery ◽  
Targeted Delivery ◽  
Viral Vectors ◽  
Large Scale ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Target Cells ◽  
Scale Production ◽  
Stepwise Development ◽  
Chimeric Peptides

Gene-based therapy largely relies on the vector type that allows a selective and efficient transfection into the target cells with maximum efficacy and minimal toxicity. Although, genes delivered utilizing modified viruses transfect efficiently and precisely, these vectors can cause severe immunological responses and are potentially carcinogenic. A promising method of overcoming this limitation is the use of non-viral vectors, including cationic lipids, polymers, dendrimers, and peptides, which offer potential routes for compacting DNA for targeted delivery. Although non-viral vectors exhibit reduced transfection efficiency compared to their viral counterpart, their superior biocompatibility, non-immunogenicity and potential for large-scale production make them increasingly attractive for modern therapy. There has been a great deal of interest in the development of biomimetic chimeric peptides. Biomimetic chimeric peptides contain different motifs for gene translocation into the nucleus of the desired cells. They have motifs for gene targeting into the desired cell, condense DNA into nanosize particles, translocate the gene into the nucleus and enhance the release of the particle into the cytoplasm. These carriers were developed in recent years. This review highlights the stepwise development of the biomimetic chimeric peptides currently being used in gene delivery.

Ring-Opening Polymerization for Hyperbranched Polycationic Gene Delivery Vectors with Excellent Serum Tolerance

2014 ◽  
Vol 6 (18) ◽  
pp. 15733-15742 ◽  
Author(s):  
Qin-Fang Zhang ◽  
Qing-Ying Yu ◽  
Yanyan Geng ◽  
Ji Zhang ◽  
Wan-Xia Wu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Ring Opening Polymerization ◽  
Gene Delivery Vectors

Gene delivery by cationic lipids: in and out of an endosome

2007 ◽  
Vol 35 (1) ◽  
pp. 68-71 ◽  
Author(s):  
D. Hoekstra ◽  
J. Rejman ◽  
L. Wasungu ◽  
F. Shi ◽  
I. Zuhorn
Keyword(s):  
Gene Delivery ◽  
Molecular Mechanisms ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Phase Changes ◽  
Model Systems ◽  
Major Pathway ◽  
Intracellular Lipids ◽  
Relative Contribution ◽  
Endosomal Membranes

Cationic lipids are exploited as vectors (‘lipoplexes’) for delivering nucleic acids, including genes, into cells for both therapeutic and cell biological purposes. However, to meet therapeutic requirements, their efficacy needs major improvement, and better defining the mechanism of entry in relation to eventual transfection efficiency could be part of such a strategy. Endocytosis is the major pathway of entry, but the relative contribution of distinct endocytic pathways, including clathrin- and caveolae-mediated endocytosis and/or macropinocytosis is as yet poorly defined. Escape of DNA/RNA from endosomal compartments is thought to represent a major obstacle. Evidence is accumulating that non-lamellar phase changes of the lipoplexes, facilitated by intracellular lipids, which allow DNA to dissociate from the vector and destabilize endosomal membranes, are instrumental in plasmid translocation into the cytosol, a prerequisite for nuclear delivery. To further clarify molecular mechanisms and to appreciate and overcome intracellular hurdles in lipoplex-mediated gene delivery, quantification of distinct steps in overall transfection and proper model systems are required.

scholarly journals Niosomes based on synthetic cationic lipids for gene delivery: the influence of polar head-groups on the transfection efficiency in HEK-293, ARPE-19 and MSC-D1 cells

2015 ◽  
Vol 13 (4) ◽  
pp. 1068-1081 ◽  
Author(s):  
E. Ojeda ◽  
G. Puras ◽  
M. Agirre ◽  
J. Zárate ◽  
S. Grijalvo ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Cationic Lipids ◽  
Head Group ◽  
Polar Head Group ◽  
Hek 293 ◽  
Polar Head ◽  
Head Groups

We designed niosomes based on three lipids that differed only in the polar-head group to analyze their influence on the transfection efficiency.

Linear polycations by ring-opening polymerization as non-viral gene delivery vectors

Biomaterials ◽  
2013 ◽  
Vol 34 (21) ◽  
pp. 5391-5401 ◽  
Author(s):  
Qin-Fang Zhang ◽  
Wen-Jing Yi ◽  
Bing Wang ◽  
Ji Zhang ◽  
Laifeng Ren ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Ring Opening Polymerization ◽  
Viral Gene ◽  
Gene Delivery Vectors ◽  
Viral Gene Delivery

CATIONIC BOLAAMPHIPHILES FOR GENE DELIVERY

COSMOS ◽  
2014 ◽  
Vol 10 (01) ◽  
pp. 25-38 ◽  
Author(s):  
AMELIA LI MIN TAN ◽  
ALISA XUE LING LIM ◽  
YITING ZHU ◽  
YI YAN YANG ◽  
MAJAD KHAN
Keyword(s):  
Gene Delivery ◽  
Viral Vectors ◽  
Serum Proteins ◽  
Transfection Efficiency ◽  
Genetic Diseases ◽  
Cationic Lipids ◽  
Nonviral Vectors ◽  
Therapeutic Gene ◽  
Natural Mechanism ◽  
High Cytotoxicity

Advances in medical research have shed light on the genetic cause of many human diseases. Gene therapy is a promising approach which can be used to deliver therapeutic genes to treat genetic diseases at its most fundamental level. In general, nonviral vectors are preferred due to reduced risk of immune response, but they are also commonly associated with low transfection efficiency and high cytotoxicity. In contrast to viral vectors, nonviral vectors do not have a natural mechanism to overcome extra- and intracellular barriers when delivering the therapeutic gene into cell. Hence, its design has been increasingly complex to meet challenges faced in targeting of, penetration of and expression in a specific host cell in achieving more satisfactory transfection efficiency. Flexibility in design of the vector is desirable, to enable a careful and controlled manipulation of its properties and functions. This can be met by the use of bolaamphiphile, a special class of lipid. Unlike conventional lipids, bolaamphiphiles can form asymmetric complexes with the therapeutic gene. The advantage of having an asymmetric complex lies in the different purposes served by the interior and exterior of the complex. More effective gene encapsulation within the interior of the complex can be achieved without triggering greater aggregation of serum proteins with the exterior, potentially overcoming one of the great hurdles faced by conventional single-head cationic lipids. In this review, we will look into the physiochemical considerations as well as the biological aspects of a bolaamphiphile-based gene delivery system.

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