Cationic polymer-derived carbon dots for enhanced gene delivery and cell imaging

2019 ◽  
Vol 7 (5) ◽  
pp. 1940-1948 ◽  
Author(s):  
Xi He ◽  
Ping Chen ◽  
Ji Zhang ◽  
Tian-Ying Luo ◽  
Hai-Jiao Wang ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Carbon Dots ◽  
Ring Opening ◽  
Cell Imaging ◽  
High Efficiency ◽  
Ring Opening Polymerization ◽  
Cationic Polymer ◽  
Cationic Polymers ◽  
Promising Strategy ◽  
Gene Vectors

The transformation of cationic polymers derived from ring-opening polymerization to carbon dots was proved as a promising strategy for developing gene vectors with high efficiency and cell-imaging ability.

scholarly journals Photoluminescent F-doped carbon dots prepared by ring-opening reaction for gene delivery and cell imaging

RSC Advances ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 6053-6062 ◽  
Author(s):  
Tian-Ying Luo ◽  
Xi He ◽  
Ji Zhang ◽  
Ping Chen ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Gene Delivery ◽  
Carbon Dots ◽  
Ring Opening ◽  
Cell Imaging ◽  
Imaging Agents ◽  
Ring Opening Reaction ◽  
Doped Carbon Dots

Carbon dots (CDs) are photoluminescent nanoparticles with distinctive properties, having great potential in nano-biomaterial systems such as gene/drug delivery vectors and cell imaging agents.

Amphiphilic polymers formed from ring-opening polymerization: a strategy for the enhancement of gene delivery

2017 ◽  
Vol 5 (4) ◽  
pp. 718-729 ◽  
Author(s):  
Yi-Mei Zhang ◽  
Zheng Huang ◽  
Ji Zhang ◽  
Wan-Xia Wu ◽  
Yan-Hong Liu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Ring Opening Polymerization ◽  
Amphiphilic Polymers ◽  
Cationic Lipids ◽  
Promising Strategy

Ring-opening polymerization was found to be a promising strategy to improve the transfection efficiency and serum tolerance of cationic lipids.

Hydrophobically modified carbon dots as a multifunctional platform for serum-resistant gene delivery and cell imaging

2020 ◽  
Vol 8 (13) ◽  
pp. 3730-3740
Author(s):  
Ping Chen ◽  
Ji Zhang ◽  
Xi He ◽  
Yan-Hong Liu ◽  
Xiao-Qi Yu
Keyword(s):  
Gene Delivery ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Activity Relationship ◽  
Viral Gene ◽  
Resistant Gene ◽  
Hydrophobically Modified ◽  
Structure Activity ◽  
Gene Vectors ◽  
Multifunctional Platform

Hydrophobically modified carbon dots were prepared and their structure-activity relationship as non-viral gene vectors was investigated.

High Efficiency Organic Lewis Pair Catalyst for Ring-Opening Polymerization of Epoxides with Chemoselectivity

2018 ◽  
Vol 51 (20) ◽  
pp. 8286-8297 ◽  
Author(s):  
Ye Chen ◽  
Jizhou Shen ◽  
Shan Liu ◽  
Junpeng Zhao ◽  
Yucai Wang ◽  
...  
Keyword(s):  
Ring Opening ◽  
High Efficiency ◽  
Ring Opening Polymerization

Diol glycidyl ether-bridged low molecular weight PEI as potential gene delivery vehicles

2015 ◽  
Vol 3 (13) ◽  
pp. 2660-2670 ◽  
Author(s):  
Qian Guo ◽  
Yan-Hong Liu ◽  
Miao-Miao Xun ◽  
Ji Zhang ◽  
Zheng Huang ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Gene Delivery ◽  
Ring Opening ◽  
Transfection Efficiency ◽  
Glycidyl Ether ◽  
Ring Opening Polymerization ◽  
Low Molecular Weight ◽  
Delivery Vehicles ◽  
Gene Delivery Vehicles

PEI 600-based polymers were synthesized via ring-opening polymerization and exhibited much better transfection efficiency and biocompatibility than PEI 25 kDa.

scholarly journals Identifying key barriers in cationic polymer gene delivery to human T cells

2019 ◽  
Vol 7 (3) ◽  
pp. 789-797 ◽  
Author(s):  
Brynn R. Olden ◽  
Emmeline Cheng ◽  
Yilong Cheng ◽  
Suzie H. Pun
Keyword(s):  
T Cells ◽  
Gene Delivery ◽  
Intracellular Ph ◽  
Cationic Polymer ◽  
Cationic Polymers ◽  
Human T Cells

Decreased uptake of polyplexes and an increased intracellular pH contribute to poor gene delivery to human T cellsviacationic polymers.

scholarly journals Hyaluronic acid-based carbon dots for efficient gene delivery and cell imaging

RSC Advances ◽  
2017 ◽  
Vol 7 (25) ◽  
pp. 15613-15624 ◽  
Author(s):  
Hai-Jiao Wang ◽  
Ji Zhang ◽  
Yan-Hong Liu ◽  
Tian-Ying Luo ◽  
Xi He ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Gene Delivery ◽  
Carbon Dots ◽  
Cell Imaging ◽  
Microwave Assisted ◽  
Efficient Gene ◽  
Microwave Assisted Method

Two polymers were used with no additives to directly construct multifunctional carbon dots by a microwave-assisted method for simultaneous gene delivery and cell imaging.

scholarly journals Enhanced transfection of a macromolecular lignin-based DNA complex with low cellular toxicity

2018 ◽  
Vol 38 (6) ◽  
Author(s):  
Yoon Khei Ho ◽  
Dan Kai ◽  
Geraldine Xue En Tu ◽  
G. Roshan Deen ◽  
Heng Phon Too ◽  
...  
Keyword(s):  
Gene Delivery ◽  
High Efficiency ◽  
Endosomal Escape ◽  
Viral Gene ◽  
Attractive Alternative ◽  
Cationic Polymers ◽  
Dna Amount ◽  
Cellular Toxicity ◽  
Molar Ratios ◽  
Post Transfection

Cationic polymers remain attractive tools for non-viral gene transfer. The effectiveness of these vectors rely on the ability to deliver plasmid DNA (pDNA) into the nucleus of cells. While we have previously demonstrated the potential of Lignin-PGEA-PEGMA as a non-viral gene delivery vector, alterations of cellular phenotype and cytotoxicity were observed post transfection. The present study aims to explore transfection conditions for high efficiency and low toxicity of the Lignin-PGEA-PEGMA based gene delivery system. Cellular toxicity was significantly reduced by using the centrifugation protocol, which enables rapid deposition of DNA complexes. Replacement of media post centrifugation resulted in minimal exposure of cells to excess polymers, which were toxic to cells. At an optimized DNA amount (500–750 ng) and molar ratios of nitrogen (N) in polymer to phosphate (P) in pDNA (N/P = 30–40), with the use of a novel transfection enhancer that facilitates endosomal escape and nuclear trafficking, the efficiency of gene delivery was increased significantly 24 h post transfection. The present study demonstrated an appropriately optimized protocol that enabled the utility of a novel cationic polymer blend with a mixture of fusogenic lipids and a histone deacetylate inhibitor in non-viral transfection, thereby providing an attractive alternative to costly commercial gene carriers.

A mitochondrial-targeting and NO-based anticancer nanosystem with enhanced photo-controllability and low dark-toxicity

2015 ◽  
Vol 3 (24) ◽  
pp. 4904-4912 ◽  
Author(s):  
Jiangsheng Xu ◽  
Fang Zeng ◽  
Hao Wu ◽  
Shuizhu Wu
Keyword(s):  
Cancer Cells ◽  
Carbon Dots ◽  
Cell Imaging ◽  
High Efficiency ◽  
Mitochondrial Targeting

A spatiotemporally controllable NO-releasing nanosystem for killing cancer cells with high efficiency based on carbon dots has been developed, which exhibits mitochondrial targeting, light-responsive NO-releasing and cell imaging capabilities.

Ring-Opening Polymerization for Hyperbranched Polycationic Gene Delivery Vectors with Excellent Serum Tolerance

2014 ◽  
Vol 6 (18) ◽  
pp. 15733-15742 ◽  
Author(s):  
Qin-Fang Zhang ◽  
Qing-Ying Yu ◽  
Yanyan Geng ◽  
Ji Zhang ◽  
Wan-Xia Wu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Ring Opening ◽  
Ring Opening Polymerization ◽  
Gene Delivery Vectors
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