Highly sensitive and selective miRNA detection based on a closed ring probe and multiple signal amplification

2016 ◽  
Vol 52 (96) ◽  
pp. 13905-13908 ◽  
Author(s):  
Yaqin Tang ◽  
Xiao He ◽  
Zhenxia Zhou ◽  
Jiakun Tang ◽  
Rong Guo ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Multiple Signal ◽  
Detection Approach ◽  
Closed Ring ◽  
Highly Sensitive ◽  
Mirna Detection ◽  
Dual Signal Amplification

A highly sensitive and selective miRNA detection approach based on a small circular probe and dual signal amplification has been reported.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Ultrasensitive and specific multi-miRNA detection based on dual signal amplification

2021 ◽  
Vol 337 ◽  
pp. 129745
Author(s):  
Ensheng Xu ◽  
Yahui Feng ◽  
Haitang Yang ◽  
Peng Li ◽  
Lu Kong ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Mirna Detection ◽  
Dual Signal Amplification

A novel electrochemical immunosensor based on dual signal amplification of gold nanoparticles and telomerase extension reaction

2014 ◽  
Vol 6 (7) ◽  
pp. 2221-2226 ◽  
Author(s):  
Xiao-Yan Li ◽  
Zi Yi ◽  
Hao Tang ◽  
Xia Chu ◽  
Ru-Qin Yu
Keyword(s):  
Gold Nanoparticles ◽  
Signal Amplification ◽  
Electrochemical Immunosensor ◽  
Highly Sensitive ◽  
Dual Signal Amplification

Highly sensitive electrochemical immune analysis was achieved based on dual signal amplification of AuNPs and telomerase extension reaction.

Electrochemical biosensor for detection of pathogenic bacteria based on dual signal amplification of Cu3(PO4)2-mediated click chemistry and DNAzyme

The Analyst ◽  
2021 ◽  
Author(s):  
Hongguo Wei ◽  
Jiayu Wan ◽  
Shengjun Bu ◽  
Wenguang Zhang ◽  
Ma Li ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Magnetic Separation ◽  
Concanavalin A ◽  
Signal Amplification ◽  
Pathogenic Bacteria ◽  
Electrochemical Biosensor ◽  
Inorganic Hybrid ◽  
Highly Sensitive ◽  
Antibody Conjugates ◽  
Dual Signal Amplification

A novel electrochemical biosensor for detecting pathogenic bacteria was designed based on specific magnetic separation and highly sensitive click chemistry. Instead of enzyme-antibody conjugates, organic-inorganic hybrid nanoflowers (Concanavalin A (Con...

Logic-signal-based multiplex detection of MiRNAs with high tension hybridization and multiple signal amplification

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4314-4320
Author(s):  
Yaqin Tang ◽  
Xiao He ◽  
Rui Yuan ◽  
Xingming Liu ◽  
Yi Zhao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Multiplex Detection ◽  
Detection Scheme ◽  
Multiple Signal ◽  
High Tension ◽  
Logic Signal ◽  
Mirna Detection ◽  
Signal Output

A multiplex miRNA detection scheme with simultaneous multiple signal output by single excitation has been reported.

Highly sensitive electrochemiluminescence aptasensor based on dual-signal amplification strategy for kanamycin detection

2020 ◽  
Vol 737 ◽  
pp. 139785
Author(s):  
Shuting Cheng ◽  
Hui Zhang ◽  
Jingcheng Huang ◽  
Rui Xu ◽  
Xia Sun ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Highly Sensitive ◽  
Amplification Strategy ◽  
Dual Signal Amplification

Ratiometric fluorescence and mesoporous structure dual signal amplification for sensitive and selective detection of TNT based on MIP@QD fluorescence sensors

2015 ◽  
Vol 51 (15) ◽  
pp. 3200-3203 ◽  
Author(s):  
Shoufang Xu ◽  
Hongzhi Lu
Keyword(s):  
Signal Amplification ◽  
Mesoporous Structure ◽  
Selective Detection ◽  
Ratiometric Fluorescence ◽  
Fluorescence Sensors ◽  
Highly Sensitive ◽  
Silica Materials ◽  
Mesoporous Silica Materials ◽  
Highly Sensitive Detection ◽  
Dual Signal Amplification

MIP@QD fluorescence sensors combined with ratiometric fluorescence techniques and mesoporous silica materials for highly sensitive detection of TNT.

A highly sensitive surface plasmon resonance sensor for the detection of DNA and cancer cells by a target-triggered multiple signal amplification strategy

2014 ◽  
Vol 50 (73) ◽  
pp. 10718-10721 ◽  
Author(s):  
Peng He ◽  
Wenping Qiao ◽  
Lijun Liu ◽  
Shusheng Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Cancer Cells ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Surface Plasmon Resonance Sensor ◽  
Multiple Signal ◽  
Highly Sensitive ◽  
Resonance Sensor ◽  
Amplification Strategy

A SPR bioassay was developed for the detection of DNA and Ramos cells by combining the target-triggered isothermal exponential amplification with MNP-based RCA.

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