Logic-signal-based multiplex detection of MiRNAs with high tension hybridization and multiple signal amplification

The Analyst ◽  
2020 ◽  
Vol 145 (12) ◽  
pp. 4314-4320
Author(s):  
Yaqin Tang ◽  
Xiao He ◽  
Rui Yuan ◽  
Xingming Liu ◽  
Yi Zhao ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Multiplex Detection ◽  
Detection Scheme ◽  
Multiple Signal ◽  
High Tension ◽  
Logic Signal ◽  
Mirna Detection ◽  
Signal Output

A multiplex miRNA detection scheme with simultaneous multiple signal output by single excitation has been reported.

Highly sensitive and selective miRNA detection based on a closed ring probe and multiple signal amplification

2016 ◽  
Vol 52 (96) ◽  
pp. 13905-13908 ◽  
Author(s):  
Yaqin Tang ◽  
Xiao He ◽  
Zhenxia Zhou ◽  
Jiakun Tang ◽  
Rong Guo ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Multiple Signal ◽  
Detection Approach ◽  
Closed Ring ◽  
Highly Sensitive ◽  
Mirna Detection ◽  
Dual Signal Amplification

A highly sensitive and selective miRNA detection approach based on a small circular probe and dual signal amplification has been reported.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Ultrasensitive and specific multi-miRNA detection based on dual signal amplification

2021 ◽  
Vol 337 ◽  
pp. 129745
Author(s):  
Ensheng Xu ◽  
Yahui Feng ◽  
Haitang Yang ◽  
Peng Li ◽  
Lu Kong ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Mirna Detection ◽  
Dual Signal Amplification

A multiple signal amplification electrochemical biosensors based on target DNA recycling for detection of the EGFR mutation status in lung cancer patients

2019 ◽  
Vol 853 ◽  
pp. 113555
Author(s):  
Xiu-Hua Weng ◽  
Xiong-Wei Xu ◽  
Pin-Fang Huang ◽  
Zhou-Jie Liu ◽  
Ai-Lin Liu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Patients ◽  
Signal Amplification ◽  
Egfr Mutation ◽  
Electrochemical Biosensors ◽  
Mutation Status ◽  
Lung Cancer Patients ◽  
Multiple Signal ◽  
Target Dna

Ultrasensitive colorimetric biosensor for BRCA1 mutation based on multiple signal amplification strategy

2020 ◽  
Vol 166 ◽  
pp. 112424
Author(s):  
Yunfei Bai ◽  
Huizhen Li ◽  
Jun Xu ◽  
Yufan Huang ◽  
Xiuming Zhang ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Brca1 Mutation ◽  
Multiple Signal ◽  
Amplification Strategy

Readout-Signal Amplification by Homodyne Detection Scheme

2007 ◽  
Author(s):  
Hideharu Mikami ◽  
Takeshi Shimano ◽  
Hiromi Kudo ◽  
Jiro Hashizume ◽  
Harukazu Miyamoto
Keyword(s):  
Signal Amplification ◽  
Homodyne Detection ◽  
Detection Scheme ◽  
Readout Signal

A highly sensitive surface plasmon resonance sensor for the detection of DNA and cancer cells by a target-triggered multiple signal amplification strategy

2014 ◽  
Vol 50 (73) ◽  
pp. 10718-10721 ◽  
Author(s):  
Peng He ◽  
Wenping Qiao ◽  
Lijun Liu ◽  
Shusheng Zhang
Keyword(s):  
Surface Plasmon Resonance ◽  
Cancer Cells ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Signal Amplification ◽  
Surface Plasmon Resonance Sensor ◽  
Multiple Signal ◽  
Highly Sensitive ◽  
Resonance Sensor ◽  
Amplification Strategy

A SPR bioassay was developed for the detection of DNA and Ramos cells by combining the target-triggered isothermal exponential amplification with MNP-based RCA.

Sign in / Sign up

Export Citation Format

Share Document