Hypouricemic effects of Mesona procumbens Hemsl. through modulating xanthine oxidase activity in vitro and in vivo

Two substances, 8-azaguanine (8AG) and 3, 3' -4, 4' -tetrahydroxy-chalcone (THC), believed to be nonoxidized in vivo inhibitors of xanthine oxidase in the rat, were used in an attempt to depress iron-release mechanisms during shock. Purine, a substrate of xanthine oxidase, was administered during shock as a means of stimulating these same mechanisms. No protection was obtained with 8AG and subsequent tests revealed that 8AG failed to depress the release of iron and uric acid both in shocked and untreated rats. The data point, instead, to inhibition of uricase by 8AG in the intact rat and not to inhibition of xanthine oxidase activity. Despite occasional depression of xanthine oxidase activity, THC exacerbated the course of traumatic shock in rats, possibly as a result of interference with pressor amine mechanisms. Purine loads were administered to normal rats and to rats primed with thorotrast. Although purine is known to elevate plasma iron in other species, rats treated with this metabolite were not unusually susceptible to lethal traumatic injury.

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In Vitro and In Vivo Studies on Adlay-Derived Seed Extracts: Phenolic Profiles, Antioxidant Activities, Serum Uric Acid Suppression, and Xanthine Oxidase Inhibitory Effects

Journal of Agricultural and Food Chemistry ◽

10.1021/jf501952e ◽

2014 ◽

Vol 62 (31) ◽

pp. 7771-7778 ◽

Cited By ~ 50

Author(s):

Mouming Zhao ◽

Dashuai Zhu ◽

Dongxiao Sun-Waterhouse ◽

Guowan Su ◽

Lianzhu Lin ◽

...

Keyword(s):

Uric Acid ◽

Xanthine Oxidase ◽

Serum Uric Acid ◽

Antioxidant Activities ◽

In Vivo Studies ◽

Inhibitory Effects ◽

Phenolic Profiles ◽

Seed Extracts

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Salvia plebeia Extract Inhibits Xanthine Oxidase Activity In Vitro and Reduces Serum Uric Acid in an Animal Model of Hyperuricemia

Planta Medica ◽

10.1055/s-0043-111012 ◽

2017 ◽

Vol 83 (17) ◽

pp. 1335-1341 ◽

Cited By ~ 3

Author(s):

Jin Kim ◽

Woo Kim ◽

Jung Hyun ◽

Jong Lee ◽

Jin Kwon ◽

...

Keyword(s):

Animal Model ◽

Enzyme Activity ◽

Uric Acid ◽

Xanthine Oxidase ◽

Serum Uric Acid ◽

Serum Urate ◽

Key Enzyme ◽

Ic50 Values

AbstractHyperuricemia is a clinical condition characterized by an elevated level of serum uric acid and is a key risk factor for the development of gout and metabolic disorders. The existing urate-lowering therapies are often impractical for certain patient populations, providing a rationale to explore new agents with improved safety and efficacy. Here, we discovered that Salvia plebeia extract inhibited the enzyme activity of xanthine oxidase, which is a key enzyme generating uric acid in the liver. In an animal model of hyperuricemia, S. plebeia extract reduced serum urate to the levels observed in control animals. The urate-lowering effect of S. plebeia extract in vivo was supported by the identification of compounds that inhibit xanthine oxidase enzyme activity in vitro. Nepetin, scutellarein, and luteolin contributed significantly to S. plebeia bioactivity in vitro. These compounds showed the highest potency against xanthine oxidase with IC50 values of 2.35, 1.74, and 1.90 µM, respectively, and were present at moderate quantities. These observations serve as a basis for further elaboration of the S. plebeia extracts for the development of new therapeutics for hyperuricemia and related diseases.

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