An automated and compartmented fluidic reactor device for multi-step sample-to-answer processes using magnetic particles

2017 ◽  
Vol 2 (3) ◽  
pp. 349-365 ◽  
Author(s):  
J. Hübner ◽  
R. Heinzler ◽  
C. Arlt ◽  
S. Hohmann ◽  
G. Brenner-Weiß ◽  
...  
Keyword(s):  
Magnetic Particles ◽  
Magnetic Particle ◽  
Particle Separation ◽  
Biotechnological Applications ◽  
Segmented Flow

A benchtop device that combines segmented flow with magnetic particle separation and active resuspension capabilities for biotechnological applications, e.g. biomolecule purification.

A laboratory and clinical evaluation of an immunochemiluminometric assay of thyrotropin in serum.

1988 ◽  
Vol 34 (10) ◽  
pp. 2087-2090 ◽  
Author(s):  
D J Berry ◽  
P M Clark ◽  
C P Price
Keyword(s):  
Magnetic Particles ◽  
Magnetic Particle ◽  
External Quality Assessment ◽  
Limit Of Detection ◽  
Particle Separation ◽  
Reference Interval ◽  
External Quality ◽  
Analytical Recovery ◽  
Luminescence Assay ◽  
Assay Time

Abstract We evaluated an immunochemiluminometric assay for human thyrotropin. A chemiluminescent acridinium ester is used as a label, with magnetic-particle separation. The lower limit of detection of the assay (mean + 3 SD of the zero standard) was 0.07 milli-int. unit/L, with a working range of 0.5 to greater than 60.0 milli-int. units/L. Assay accuracy was good as judged from analytical recovery, analysis of external quality-assessment samples, and comparison with an enzyme-amplified immunoassay. There were no significant interferences or cross-reactivities. Twenty-four samples assayed showed aggregation of the magnetic particles. On re-assay, four of these samples showed a significant increase in the measured TSH by the luminescence assay. Assay time for 60 tubes was approximately 3.5 h with the use of a semi-automated luminometer. The reference interval, determined from data on 144 healthy euthyroid subjects, was 0.3-4.0 milli-int. units/L. Sixteen of 19 thyrotoxic patients showed clearly suppressed concentrations of thyrotropin in serum.

scholarly journals Optical Imaging of Magnetic Particle Cluster Oscillation and Rotation in Glycerol

2021 ◽  
Vol 7 (5) ◽  
pp. 82
Author(s):  
River Gassen ◽  
Dennis Thompkins ◽  
Austin Routt ◽  
Philippe Jones ◽  
Meghan Smith ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Barium Hexaferrite ◽  
Optical Microscope ◽  
Simplified Model ◽  
Particle Cluster ◽  
Average Deviation ◽  
Cross Sectional

Magnetic particles have been evaluated for their biomedical applications as a drug delivery system to treat asthma and other lung diseases. In this study, ferromagnetic barium hexaferrite (BaFe12O19) and iron oxide (Fe3O4) particles were suspended in water or glycerol, as glycerol can be 1000 times more viscous than water. The particle concentration was 2.50 mg/mL for BaFe12O19 particle clusters and 1.00 mg/mL for Fe3O4 particle clusters. The magnetic particle cluster cross-sectional area ranged from 15 to 1000 μμm2, and the particle cluster diameter ranged from 5 to 45 μμm. The magnetic particle clusters were exposed to oscillating or rotating magnetic fields and imaged with an optical microscope. The oscillation frequency of the applied magnetic fields, which was created by homemade wire spools inserted into an optical microscope, ranged from 10 to 180 Hz. The magnetic field magnitudes varied from 0.25 to 9 mT. The minimum magnetic field required for particle cluster rotation or oscillation in glycerol was experimentally measured at different frequencies. The results are in qualitative agreement with a simplified model for single-domain magnetic particles, with an average deviation from the model of 1.7 ± 1.3. The observed difference may be accounted for by the fact that our simplified model does not include effects on particle cluster motion caused by randomly oriented domains in multi-domain magnetic particle clusters, irregular particle cluster size, or magnetic anisotropy, among other effects.

Development of a bacterial DNA extraction modular chip using a magnetic particle and portable vibration motor

2020 ◽  
Vol 12 (9) ◽  
pp. 1197-1202
Author(s):  
Sun Young Lim ◽  
Tae Jae Lee ◽  
Seol Yi Shin ◽  
Nam Ho Bae ◽  
Seok Jae Lee ◽  
...  
Keyword(s):  
Power System ◽  
Electric Power ◽  
Dna Extraction ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Electric Power System ◽  
Bacterial Dna ◽  
System P ◽  
3D Printed ◽  
Bacterial Dna Extraction

The bacterial DNA was simply purified by magnetic particles with a portable vibration motor. To effectively extract DNA in the field, the 3D-printed device was employed with low electric power system.

Gold Magnetic Particle Based Immunoassay on a Disposable Microchips

2011 ◽  
Vol 236-238 ◽  
pp. 1931-1934
Author(s):  
Zi Ye Wang ◽  
Kang Wang ◽  
Xue Mei Ma
Keyword(s):  
Magnetic Nanoparticles ◽  
Reaction Time ◽  
Laser Ablation ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Binding Assay ◽  
Igg Binding

Microchips appear to offer important opportunities for modern research. Microchips based on gold magnetic nanoparticles make manipulation of biomolecules more conveniently, and has the advantage of deducing reaction time. In this study, we first achieved microchips design and fabrication through CO2 laser ablation for immunoassay, and then conjugated mouse IgG to gold magnetic nanoparticles serve as a target. Finally, HRP labeled goat anti mouse IgG binding assay and substrate reaction were performed with disposable microchips and other devices. The results indicated that the reactions carried on successfully and magnetic particles moved well in chips.

Synthesis of transparent magnetic particle/organic hybrid film using iron–organics

2000 ◽  
Vol 15 (10) ◽  
pp. 2114-2120 ◽  
Author(s):  
Toshinobu Yogo ◽  
Tomoyuki Nakamura ◽  
Wataru Sakamoto ◽  
Shin-ichi Hirano
Keyword(s):  
Iron Oxide ◽  
Magnetization Curve ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Hybrid Film ◽  
Oxide Particle ◽  
Iron Oxide Particle ◽  
Organic Hybrid ◽  
Hydrolysis Conditions

A transparent magnetic particle/organic film was synthesized from an iron–organic compound. Iron(III) 3-allylacetylacetonate (IAA) was polymerized followed by in situ hydrolysis yielding an iron oxide particle/oligomer hybrid. The sizes of magnetic particles were dependent upon the hydrolysis conditions of the IAA oligomers. A nanometer-sized ferrimagnetic iron oxide particle/oligomer hybrid showed a magnetization curve with no coercive force at 300 K and that with Hc of 200 Oe at 4.2 K, respectively. The magnetization versus H/T curves at 300 and 77 K were superimposed on each other and satisfied the Langevin equation. The transparent hybrid film showed a magnetization curve at room temperature. The absorption spectrum of the film was shifted to higher energy by 0.14 eV compared with that of bulk magnetite. The absorption edge of the film was blue-shifted.

scholarly journals Reviewing Magnetic Particle Preparation: Exploring the Viability in Biosensing

Sensors ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 4596
Author(s):  
Daniel Kappe ◽  
Laila Bondzio ◽  
Joris Swager ◽  
Andreas Becker ◽  
Björn Büker ◽  
...  
Keyword(s):  
Magnetic Particles ◽  
Magnetic Particle ◽  
Size Range ◽  
Particle System ◽  
Microfluidic Channel ◽  
Review Article ◽  
Particle Systems ◽  
Heusler Compound ◽  
Particle Preparation ◽  
New Applications

In this review article, we conceptually investigated the requirements of magnetic nanoparticles for their application in biosensing and related them to example systems of our thin-film portfolio. Analyzing intrinsic magnetic properties of different magnetic phases, the size range of the magnetic particles was determined, which is of potential interest for biosensor technology. Different e-beam lithography strategies are utilized to identify possible ways to realize small magnetic particles targeting this size range. Three different particle systems from 500 μm to 50 nm are produced for this purpose, aiming at tunable, vertically magnetized synthetic antiferromagnets, martensitic transformation in a single elliptical, disc-shaped Heusler Ni50Mn32.5Ga17.5 particle and nanocylinders of Co2MnSi-Heusler compound. Perspectively, new applications for these particle systems in combination with microfluidics are addressed. Using the concept of a magnetic on–off ratchet, the most suitable particle system of these three materials is validated with respect to magnetically-driven transport in a microfluidic channel. In addition, options are also discussed for improving the magnetic ratchet for larger particles.

Sign in / Sign up

Export Citation Format

Share Document