Gold Magnetic Particle Based Immunoassay on a Disposable Microchips

2011 ◽  
Vol 236-238 ◽  
pp. 1931-1934
Author(s):  
Zi Ye Wang ◽  
Kang Wang ◽  
Xue Mei Ma
Keyword(s):  
Magnetic Nanoparticles ◽  
Reaction Time ◽  
Laser Ablation ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Binding Assay ◽  
Igg Binding

Microchips appear to offer important opportunities for modern research. Microchips based on gold magnetic nanoparticles make manipulation of biomolecules more conveniently, and has the advantage of deducing reaction time. In this study, we first achieved microchips design and fabrication through CO2 laser ablation for immunoassay, and then conjugated mouse IgG to gold magnetic nanoparticles serve as a target. Finally, HRP labeled goat anti mouse IgG binding assay and substrate reaction were performed with disposable microchips and other devices. The results indicated that the reactions carried on successfully and magnetic particles moved well in chips.

scholarly journals Optical Imaging of Magnetic Particle Cluster Oscillation and Rotation in Glycerol

2021 ◽  
Vol 7 (5) ◽  
pp. 82
Author(s):  
River Gassen ◽  
Dennis Thompkins ◽  
Austin Routt ◽  
Philippe Jones ◽  
Meghan Smith ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Magnetic Fields ◽  
Magnetic Particles ◽  
Magnetic Particle ◽  
Barium Hexaferrite ◽  
Optical Microscope ◽  
Simplified Model ◽  
Particle Cluster ◽  
Average Deviation ◽  
Cross Sectional

Magnetic particles have been evaluated for their biomedical applications as a drug delivery system to treat asthma and other lung diseases. In this study, ferromagnetic barium hexaferrite (BaFe12O19) and iron oxide (Fe3O4) particles were suspended in water or glycerol, as glycerol can be 1000 times more viscous than water. The particle concentration was 2.50 mg/mL for BaFe12O19 particle clusters and 1.00 mg/mL for Fe3O4 particle clusters. The magnetic particle cluster cross-sectional area ranged from 15 to 1000 μμm2, and the particle cluster diameter ranged from 5 to 45 μμm. The magnetic particle clusters were exposed to oscillating or rotating magnetic fields and imaged with an optical microscope. The oscillation frequency of the applied magnetic fields, which was created by homemade wire spools inserted into an optical microscope, ranged from 10 to 180 Hz. The magnetic field magnitudes varied from 0.25 to 9 mT. The minimum magnetic field required for particle cluster rotation or oscillation in glycerol was experimentally measured at different frequencies. The results are in qualitative agreement with a simplified model for single-domain magnetic particles, with an average deviation from the model of 1.7 ± 1.3. The observed difference may be accounted for by the fact that our simplified model does not include effects on particle cluster motion caused by randomly oriented domains in multi-domain magnetic particle clusters, irregular particle cluster size, or magnetic anisotropy, among other effects.

Debye-Based Frequency-Domain Magnetization Model for Magnetic Nanoparticles in Magnetic Particle Spectroscopy

2015 ◽  
Vol 51 (2) ◽  
pp. 1-4 ◽  
Author(s):  
Thilo Wawrzik ◽  
Takashi Yoshida ◽  
Meinhard Schilling ◽  
Frank Ludwig
Keyword(s):  
Magnetic Nanoparticles ◽  
Frequency Domain ◽  
Magnetic Particle

scholarly journals Introducing a frequency-tunable magnetic particle spectrometer

2015 ◽  
Vol 1 (1) ◽  
pp. 249-253 ◽  
Author(s):  
André Behrends ◽  
Matthias Graeser ◽  
Thorsten M. Buzug
Keyword(s):  
Magnetic Nanoparticles ◽  
Image Quality ◽  
Magnetic Particle ◽  
Imaging Modality ◽  
Magnetic Particle Imaging ◽  
Common Technique ◽  
Frequency Tunable ◽  
Excitation Frequencies ◽  
Particle Imaging

AbstractImage quality in the new imaging modality magnetic particle imaging (MPI) heavily relies on the quality of the magnetic nanoparticles in use. Therefore, it is crucial to understand the behaviour of such particles. A common technique to analyze the behaviour of the particles is magnetic particle spectrometry (MPS). However, most spectrometers are limited to measurements at a single or multiple discrete excitation frequencies. This paper introduces a frequency-tunable spectrometer, able to perform measurements in the range of 100 Hz - 24kHz.

scholarly journals Modelling the concentration of anti-SARS-CoV-2 immunoglobulin G in intravenous immunoglobulin product batches

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259731
Author(s):  
Sara Stinca ◽  
Thomas W. Barnes ◽  
Peter Vogel ◽  
Wilfried Meyers ◽  
Johannes Schulte-Pelkum ◽  
...  
Keyword(s):  
Intravenous Immunoglobulin ◽  
Binding Assay ◽  
Elevated Concentration ◽  
Plasma Pool ◽  
Igg Binding ◽  
Donor Population ◽  
The Mean ◽  
Antibody Levels ◽  
Design And Methods ◽  
Ig G

Background Plasma-derived intravenous immunoglobulin (IVIg) products contain a dynamic spectrum of immunoglobulin (Ig) G reactivities reflective of the donor population from which they are derived. We sought to model the concentration of anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) IgG which could be expected in future plasma pool and final-product batches of CSL Behring’s immunoglobulin product Privigen. Study design and methods Data was extracted from accessible databases, including the incidence of coronavirus disease 2019 and SARS-CoV-2 vaccination status, antibody titre in convalescent and vaccinated groups and antibody half-life. Together, these parameters were used to create an integrated mathematical model that could be used to predict anti-SARS-CoV-2 antibody levels in future IVIg preparations. Results We predict that anti-SARS-CoV-2 IgG concentration will peak in batches produced in mid-October 2021, containing levels in the vicinity of 190-fold that of the mean convalescent (unvaccinated) plasma concentration. An elevated concentration (approximately 35-fold convalescent plasma) is anticipated to be retained in batches produced well into 2022. Measurement of several Privigen batches using the Phadia™ EliA™ SARS-CoV-2-Sp1 IgG binding assay confirmed the early phase of this model. Conclusion The work presented in this paper may have important implications for physicians and patients who use Privigen for indicated diseases.

scholarly journals Novel Benchtop Magnetic Particle Spectrometer for Process Monitoring of Magnetic Nanoparticle Synthesis

Nanomaterials ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 2277
Author(s):  
Norbert Löwa ◽  
Dirk Gutkelch ◽  
Ernst-Albrecht Welge ◽  
Roland Welz ◽  
Florian Meier ◽  
...  
Keyword(s):  
Magnetic Properties ◽  
Magnetic Nanoparticles ◽  
Process Monitoring ◽  
Magnetic Nanoparticle ◽  
Magnetic Particle ◽  
Nanoparticle Synthesis ◽  
Separation Processes ◽  
Magnetic Detector ◽  
Application Fields

Magnetic nanoparticles combine unique magnetic properties that can be used in a variety of biomedical applications for therapy and diagnostics. These applications place high demands on the magnetic properties of nanoparticles. Thus, research, development, and quality assurance of magnetic nanoparticles requires powerful analytical methods that are capable of detecting relevant structural and, above all, magnetic parameters. By directly coupling nanoparticle synthesis with magnetic detectors, relevant nanoparticle properties can be obtained and evaluated, and adjustments can be made to the manufacturing process in real time. This work presents a sensitive and fast magnetic detector for online characterization of magnetic nanoparticles during their continuous micromixer synthesis. The detector is based on the measurement of the nonlinear dynamic magnetic response of magnetic nanoparticles exposed to an oscillating excitation at a frequency of 25 kHz, a technique also known as magnetic particle spectroscopy. Our results underline the excellent suitability of the developed magnetic online detection for coupling with magnetic nanoparticle synthesis based on the micromixer approach. The proven practicability and reliability of the detector for process monitoring forms the basis for further application fields, e.g., as a monitoring tool for chromatographic separation processes.

scholarly journals Micromixer Synthesis Platform for a Tuneable Production of Magnetic Single-Core Iron Oxide Nanoparticles

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1845
Author(s):  
Abdulkader Baki ◽  
Norbert Löwa ◽  
Amani Remmo ◽  
Frank Wiekhorst ◽  
Regina Bleul
Keyword(s):  
Iron Oxide ◽  
Magnetic Nanoparticles ◽  
Iron Oxide Nanoparticles ◽  
Magnetic Particle ◽  
Biomedical Application ◽  
Ac Susceptibility ◽  
Chemical Properties ◽  
Oxide Nanoparticles ◽  
Aqueous Synthesis ◽  
Characterization Methods

Micromixer technology is a novel approach to manufacture magnetic single-core iron oxide nanoparticles that offer huge potential for biomedical applications. This platform allows a continuous, scalable, and highly controllable synthesis of magnetic nanoparticles with biocompatible educts via aqueous synthesis route. Since each biomedical application requires specific physical and chemical properties, a comprehensive understanding of the synthesis mechanisms is not only mandatory to control the size and shape of desired nanoparticle systems but, above all, to obtain the envisaged magnetic particle characteristics. The accurate process control of the micromixer technology can be maintained by adjusting two parameters: the synthesis temperature and the residence time. To this end, we performed a systematic variation of these two control parameters synthesizing magnetic nanoparticle systems, which were analyzed afterward by structural (transmission electron microscopy and differential sedimentation centrifugation) and, especially, magnetic characterization methods (magnetic particle spectroscopy and AC susceptibility). Furthermore, we investigated the reproducibility of the microtechnological nanoparticle manufacturing process compared to batch preparation. Our characterization demonstrated the high magnetic quality of single-core iron oxide nanoparticles with core diameters in the range of 20 nm to 40 nm synthesized by micromixer technology. Moreover, we demonstrated the high capability of a newly developed benchtop magnetic particle spectroscopy device that directly monitored the magnetic properties of the magnetic nanoparticles with the highest sensitivity and millisecond temporal resolution during continuous micromixer synthesis.

First Sustainable Aziridination of Olefins Using Recyclable Copper-Immobilized Magnetic Nanoparticles

Synlett ◽  
2019 ◽  
Vol 30 (05) ◽  
pp. 563-566 ◽  
Author(s):  
Sylvestre Toumieux ◽  
Mohamad Khodadadi ◽  
Gwladys Pourceau ◽  
Matthieu Becuwe ◽  
Anne Wadouachi
Keyword(s):  
Magnetic Nanoparticles ◽  
Reaction Time ◽  
Microwave Irradiation ◽  
Significant Loss ◽  
Conventional Heating ◽  
Heating Method ◽  
Magnetic Extraction

The first copper-catalyzed aziridination of olefins using re­cyclable magnetic nanoparticles is described. Magnetic nanoparticles were modified with dopamine and used as a support to coordinate copper. The methodology was optimized with styrene as olefin and using [N-(p-toluenesulfonyl)imino]phenyliodinane (PhI=NTs) as nitrene source. A microwave irradiation decreased the reaction time by 4-fold compared to conventional heating method. The catalyst was recovered by simple magnetic extraction and could be reused successfully up to five times without significant loss of activity. The methodology was ­applied to a range of different olefins leading to moderate to excellent yields in the formation of the expected aziridine.

Optimization of Magnetic Nanoparticles for Magnetic Particle Imaging

2012 ◽  
Vol 48 (11) ◽  
pp. 3780-3783 ◽  
Author(s):  
Frank Ludwig ◽  
Thilo Wawrzik ◽  
Takashi Yoshida ◽  
Nicole Gehrke ◽  
Andreas Briel ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Magnetic Particle ◽  
Magnetic Particle Imaging ◽  
Particle Imaging
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