A pure DNA hydrogel with stable catalytic ability produced by one-step rolling circle amplification

2017 ◽  
Vol 53 (21) ◽  
pp. 3038-3041 ◽  
Author(s):  
Yishun Huang ◽  
Wanlin Xu ◽  
Guoyuan Liu ◽  
Leilei Tian
Keyword(s):  
Rolling Circle Amplification ◽  
Cost Effective ◽  
Rolling Circle ◽  
Catalytic Stability ◽  
One Step ◽  
Facile Fabrication ◽  
Dna Hydrogel

Rolling circle amplification for cost-effective and facile fabrication of a pure DNA hydrogel with highly improved catalytic stability.

scholarly journals Rapid electrochemical detection of coronavirus SARS-CoV-2

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

AbstractCoronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

One-step discrimination of BCR/ABLp210 transcript isoforms directly from RNA extraction with fusion-triggered rolling circle amplification

2019 ◽  
Vol 1067 ◽  
pp. 129-136 ◽  
Author(s):  
Nini Luo ◽  
Qianfeng Xia ◽  
Lutan Zhang ◽  
Yuhong Zhang ◽  
Lizhen Huang ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Rna Extraction ◽  
Transcript Isoforms ◽  
Rolling Circle ◽  
One Step

scholarly journals High-throughput, cost-effective verification of structural DNA assembly

2013 ◽  
Vol 42 (4) ◽  
pp. e22-e22 ◽  
Author(s):  
Yandi Dharmadi ◽  
Kedar Patel ◽  
Elaine Shapland ◽  
Daniel Hollis ◽  
Todd Slaby ◽  
...  
Keyword(s):  
High Throughput ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
Cost Effective ◽  
Building Blocks ◽  
Pathway Engineering ◽  
Dna Assembly ◽  
Rolling Circle ◽  
Enzyme Screening ◽  
First Time

Abstract DNA ‘assembly’ from ‘building blocks’ remains a cornerstone in synthetic biology, whether it be for gene synthesis (∼1 kb), pathway engineering (∼10 kb) or synthetic genomes (>100 kb). Despite numerous advances in the techniques used for DNA assembly, verification of the assembly is still a necessity, which becomes cost-prohibitive and a logistical challenge with increasing scale. Here we describe for the first time a comprehensive, high-throughput solution for structural DNA assembly verification by restriction digest using exhaustive in silico enzyme screening, rolling circle amplification of plasmid DNA, capillary electrophoresis and automated digest pattern recognition. This low-cost and robust methodology has been successfully used to screen over 31 000 clones of DNA constructs at <$1 per sample.

DNA Hydrogel with Tunable pH-Responsive Properties Produced by Rolling Circle Amplification

2017 ◽  
Vol 23 (72) ◽  
pp. 18276-18281 ◽  
Author(s):  
Wanlin Xu ◽  
Yishun Huang ◽  
Haoran Zhao ◽  
Pan Li ◽  
Guoyuan Liu ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ph Responsive ◽  
Rolling Circle ◽  
Dna Hydrogel

scholarly journals Ultrafast, one-step, and microwave heating-based synthesis of DNA/RNA-AuNP conjugates

2021 ◽  
Author(s):  
Mengqi Huang ◽  
Erhu Xiong ◽  
Menglu Hu ◽  
Huahua Yue ◽  
Tian Tian ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Rolling Circle Amplification ◽  
Test Strip ◽  
N Gene ◽  
Great Promise ◽  
Rolling Circle ◽  
Spherical Nucleic Acid ◽  
Standing Up ◽  
One Step ◽  
Nucleic Acids Delivery

DNA/RNA-gold nanoparticle (DNA/RNA-AuNP) nanoprobes have been widely employed for nanobiotechnology applications. Here we discovered that both thiolated and non-thiolated DNA/RNA can be efficiently attached to AuNPs to achieve high-stable spherical nucleic acid (SNA) within minutes under a domestic microwave (MW)-assisted heating-dry circumstance. Further studies showed that for non-thiolated DNA/RNA the conjugation is poly (T/U) tag dependent. Spectroscopy, test strip hybridization, and loading counting experiments indicate that low-affinity poly (T/U) tag mediates the formation of a standing-up conformation, which is distributed in the outer layer of such a SNA structure. In further applications study, CRISPR/Cas9-sgRNA (135 bp), RNA from Nucleocapsid (N) gene of SARS-CoV-2 (1279 bp), and rolling circle amplification (RCA) DNA products (over 1000 bp) could be successfully attached on AuNPs, which overcomes the routine methods in long-chain nucleic acid-AuNP conjugation, exhibiting great promise in novel biosensing and nucleic acids delivery strategy. This novel heating-dry strategy has improved the traditional DNA/RNA-AuNP conjugation methods in simplicity, rapidity, cost, and universality.

Base excision repair initiated rolling circle amplification-based fluorescent assay for screening uracil-DNA glycosylase activity using Endo IV-assisted cleavage of AP probes

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3951-3958 ◽  
Author(s):  
Jingfeng Wang ◽  
Yu Wang ◽  
Su Liu ◽  
Haiwang Wang ◽  
Xue Zhang ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Signal Amplification ◽  
Excision Repair ◽  
Rolling Circle Amplification ◽  
Cost Effective ◽  
Ultrasensitive Detection ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Rolling Circle ◽  
Base Excision

A simple, robust and cost effective biosensing platform for the ultrasensitive detection of UDG activity was established based on base excision repair-initiated primer generation for RCA with Endo IV-assisted signal amplification.

scholarly journals Rapid Electrochemical Detection of Coronavirus SARS-CoV-2.

2020 ◽  
Author(s):  
Thanyarat Chaibun ◽  
Jiratchaya Puenpa ◽  
Tatchanun Ngamdee ◽  
Nimaradee Boonapatcharoen ◽  
Pornpat Athamanolap ◽  
...  
Keyword(s):  
Electrochemical Biosensor ◽  
Rolling Circle Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Qrt Pcr ◽  
Reverse Transcription Pcr ◽  
Sandwich Hybridization ◽  
Redox Active ◽  
One Step ◽  
The One

Abstract COVID-19 is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/mL of N and S genes, in less than 2 hours. Sensor evaluation with 105 clinical samples, including 40 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.

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