scholarly journals A Red Emissive Fluorescent Turn-on Sensor for the Rapid Detection of Selenocysteine and Its Application in Living Cells Imaging

Sensors ◽  
2020 ◽  
Vol 20 (17) ◽  
pp. 4768 ◽  
Author(s):  
Zongcheng Wang ◽  
Huihuang Zheng ◽  
Chengliang Zhang ◽  
Dongfang Tang ◽  
Qiyao Wu ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
High Sensitivity ◽  
Living Cells ◽  
Fluorescent Sensors ◽  
Quantitative Detection ◽  
Fast Reaction ◽  
Detection Range ◽  
Long Wavelength ◽  
Turn On

The content of selenocysteine in cells has an important effect on a variety of human diseases, and the detection of selenocysteine by fluorescent sensors in vivo has shown many advantages. In order to further develop fast-reaction-time, good-selectivity, and high-sensitivity long-wavelength selenocysteine fluorescent sensors, we designed and synthesized the compound YZ-A4 as a turn-on fluorescent sensor to detect the content of selenocysteine. The quantitative detection range of the sensor YZ-A4 to selenocysteine was from 0 to 32 μM, and the detection limit was as low as 11.2 nM. The sensor displayed a rapid turn-on response, good selectivity, and high sensitivity to selenocysteine. Finally, we have demonstrated that YZ-A4 could be used for fluorescence imaging of selenocysteine in living cells.

A water-soluble BODIPY derivative as a highly selective “Turn-On” fluorescent sensor for H2O2 sensing in vivo

2014 ◽  
Vol 56 ◽  
pp. 58-63 ◽  
Author(s):  
Jian Xu ◽  
Qian Li ◽  
Ying Yue ◽  
Yong Guo ◽  
Shijun Shao
Keyword(s):  
Fluorescent Sensor ◽  
Water Soluble ◽  
Turn On ◽  
H2o2 Sensing

The development of a hemicyanine-based ratiometric CO fluorescent probe with a long emission wavelength and its applications for imaging CO in vitro and in vivo

2020 ◽  
Vol 44 (28) ◽  
pp. 12107-12112 ◽  
Author(s):  
Yunyan Zhang ◽  
Xiuqi Kong ◽  
Yonghe Tang ◽  
Min Li ◽  
Yaguang Yin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Emission Wavelength ◽  
Long Wavelength ◽  
Wavelength Emission ◽  
Ratiometric Fluorescent Probe

A novel ratiometric fluorescent probe, Hcy-CO, with long-wavelength emission was developed for visualizing CO in living cells and zebrafish.

scholarly journals Quantification of Neuropeptide Y with Picomolar Sensitivity Enabled by Guided-Mode Resonance Biosensors

Sensors ◽  
2019 ◽  
Vol 20 (1) ◽  
pp. 126 ◽  
Author(s):  
Mohammad G. Abdallah ◽  
Joseph A. Buchanan-Vega ◽  
Kyu J. Lee ◽  
Brett R. Wenner ◽  
Jeffery W. Allen ◽  
...  
Keyword(s):  
Neuropeptide Y ◽  
Near Infrared ◽  
Single Point ◽  
High Sensitivity ◽  
Quantitative Detection ◽  
Label Free ◽  
Sensor Surface ◽  
Detection Range ◽  
Guided Mode ◽  
Guided Mode Resonance

Assessing levels of neuropeptide Y (NPY) in the human body has many medical uses. Accordingly, we report the quantitative detection of NPY biomarkers applying guided-mode resonance (GMR) biosensor methodology. The label-free sensor operates in the near-infrared spectral region exhibiting distinctive resonance signatures. The interaction of NPY with bioselective molecules on the sensor surface causes spectral shifts that directly identify the binding event without additional processing. In the experiments described here, NPY antibodies are attached to the sensor surface to impart specificity during operation. For the low concentrations of NPY of interest, we apply a sandwich NPY assay in which the sensor-linked anti-NPY molecule binds with NPY that subsequently binds with anti-NPY to close the sandwich. The sandwich assay achieves a detection limit of ~0.1 pM NPY. The photonic sensor methodology applied here enables expeditious high-throughput data acquisition with high sensitivity and specificity. The entire bioreaction is recorded as a function of time, in contrast to label-based methods with single-point detection. The convenient methodology and results reported are significant, as the NPY detection range of 0.1–10 pM demonstrated is useful in important medical circumstances.

A selective turn-on fluorescent sensor for Cu(II) and its application in imaging in living cells

2010 ◽  
Vol 148 (1) ◽  
pp. 337-341 ◽  
Author(s):  
Pinxian Xi ◽  
Jinyan Dou ◽  
Liang Huang ◽  
Ming Xu ◽  
Fengjuan Chen ◽  
...  
Keyword(s):  
Fluorescent Sensor ◽  
Living Cells ◽  
Turn On

A highly selective turn-on fluorescent sensor for fluoride and its application in imaging of living cells

2014 ◽  
Vol 38 (12) ◽  
pp. 6198-6204 ◽  
Author(s):  
Jiun-Ting Yeh ◽  
Parthiban Venkatesan ◽  
Shu-Pao Wu
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Enhancement ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Green Fluorescence ◽  
Turn On ◽  
Highly Sensitive

A highly sensitive and selective fluorescent probe (FS) is based on a fluoride-specific desilylation reaction to yield a significant green fluorescence enhancement.

A long-wavelength-emitting fluorescent turn-on probe for imaging hydrogen sulfide in living cells

2014 ◽  
Vol 202 ◽  
pp. 99-104 ◽  
Author(s):  
Changquan Tang ◽  
Qingdong Zheng ◽  
Shenfei Zong ◽  
Zhuyuan Wang ◽  
Yiping Cui
Keyword(s):  
Hydrogen Sulfide ◽  
Living Cells ◽  
Long Wavelength ◽  
Turn On

Pyrene appended thymine derivative for selective turn-on fluorescence sensing of uric acid in live cells

RSC Advances ◽  
2016 ◽  
Vol 6 (71) ◽  
pp. 66774-66778 ◽  
Author(s):  
Prithidipa Sahoo ◽  
Himadri Sekhar Sarkar ◽  
Sujoy Das ◽  
Kalipada Maiti ◽  
Md Raihan Uddin ◽  
...  
Keyword(s):  
Uric Acid ◽  
Fluorescent Probe ◽  
High Sensitivity ◽  
Living Cells ◽  
Live Cells ◽  
Fluorescence Sensing ◽  
Turn On ◽  
Sensitivity And Selectivity ◽  
First Time

A new ‘turn-ON’ fluorescent probe, pyrene appended thymine acetamide (PTA), with high sensitivity and selectivity for the detection of uric acid (UA) was developed and first time imaging of uric acid in living cells in water was achieved.

A novel turn-on Schiff-base fluorescent sensor for aluminum(iii) ions in living cells

RSC Advances ◽  
2015 ◽  
Vol 5 (129) ◽  
pp. 107012-107019 ◽  
Author(s):  
Jianfei Tian ◽  
Xiaxia Yan ◽  
Hui Yang ◽  
Fuli Tian
Keyword(s):  
Schiff Base ◽  
Metal Ions ◽  
High Selectivity ◽  
Fluorescence Enhancement ◽  
Fluorescent Sensor ◽  
Living Cells ◽  
Excitation Wavelength ◽  
Turn On

Quinoline Schiff base L exhibits a more than 38-fold fluorescence enhancement after addition of Al3+ ions at an excitation wavelength of 373 nm, and shows a high selectivity for Al3+ over other metal ions.

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