A microfluidic platform utilizing anchored water-in-oil-in-water double emulsions to create a niche for analyzing single non-adherent cells

Lab on a Chip ◽  
2019 ◽  
Vol 19 (3) ◽  
pp. 422-431 ◽  
Author(s):  
Bo Cai ◽  
Tian-Tian Ji ◽  
Ning Wang ◽  
Xin-Bo Li ◽  
Rong-Xiang He ◽  
...  
Keyword(s):  
Adherent Cells ◽  
Microfluidic Platform ◽  
Double Emulsions ◽  
Oil In Water ◽  
On Chip

Water-in-oil-in-water double emulsions (W/O/W DEs) are generated to encapsulate non-adherent cells and anchored in an array on-chip for in situ assays.

scholarly journals Laser-excited elastic guided waves reveal the complex mechanics of nanoporous silicon

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Marc Thelen ◽  
Nicolas Bochud ◽  
Manuel Brinker ◽  
Claire Prada ◽  
Patrick Huber
Keyword(s):  
Guided Waves ◽  
Laser Ultrasonics ◽  
Bulk Silicon ◽  
Nanoporous Silicon ◽  
Stiffness Reduction ◽  
Mechanical Characterisation ◽  
Isotropic Elasticity ◽  
On Chip ◽  
Non Destructive

AbstractNanoporosity in silicon leads to completely new functionalities of this mainstream semiconductor. A difficult to assess mechanics has however significantly limited its application in fields ranging from nanofluidics and biosensorics to drug delivery, energy storage and photonics. Here, we present a study on laser-excited elastic guided waves detected contactless and non-destructively in dry and liquid-infused single-crystalline porous silicon. These experiments reveal that the self-organised formation of 100 billions of parallel nanopores per square centimetre cross section results in a nearly isotropic elasticity perpendicular to the pore axes and an 80% effective stiffness reduction, altogether leading to significant deviations from the cubic anisotropy observed in bulk silicon. Our thorough assessment of the wafer-scale mechanics of nanoporous silicon provides the base for predictive applications in robust on-chip devices and evidences that recent breakthroughs in laser ultrasonics open up entirely new frontiers for in-situ, non-destructive mechanical characterisation of dry and liquid-functionalised porous materials.

scholarly journals Controlled density glycodendron microarrays for studying carbohydrate–lectin interactions

2021 ◽  
Author(s):  
Antonio Di Maio ◽  
Anna Cioce ◽  
Silvia Achilli ◽  
Michel Thépaut ◽  
Corinne Vivès ◽  
...  
Keyword(s):  
Maldi Tof Ms ◽  
Maldi Tof ◽  
On Chip ◽  
Tof Ms

Density depended binding and selectivity is studied on glycodendron microarray with defined valency, which were prepared by on-chip synthesis and analysed by in situ MALDI-TOF MS.

Encapsulating Tin Nanoflowers into Microcapsules for High‐Rate‐Performance Secondary Battery Anodes through In Situ Polymerizing Oil‐in‐Water Interface

2020 ◽  
Vol 8 (5) ◽  
pp. 1901404 ◽  
Author(s):  
Tianli Han ◽  
Yong Wu ◽  
Yingyi Ding ◽  
Yan Zhong ◽  
Ping Zhou ◽  
...  
Keyword(s):  
High Rate ◽  
Water Interface ◽  
Rate Performance ◽  
Secondary Battery ◽  
Oil In Water ◽  
High Rate Performance

In-situ control of on-chip angstrom gaps, atomic switches, and molecular junctions by light irradiation

Nano Today ◽  
2021 ◽  
Vol 39 ◽  
pp. 101226
Author(s):  
Surong Zhang ◽  
Chenyang Guo ◽  
Lifa Ni ◽  
Kerstin M. Hans ◽  
Weiqiang Zhang ◽  
...  
Keyword(s):  
Molecular Junctions ◽  
Light Irradiation ◽  
In Situ Control ◽  
On Chip

A novel microfluidic platform with stable concentration gradient for on chip cell culture and screening assays

Lab on a Chip ◽  
2013 ◽  
Vol 13 (18) ◽  
pp. 3714 ◽  
Author(s):  
Bi-Yi Xu ◽  
Shan-Wen Hu ◽  
Guang-Sheng Qian ◽  
Jing-Juan Xu ◽  
Hong-Yuan Chen
Keyword(s):  
Cell Culture ◽  
Concentration Gradient ◽  
Microfluidic Platform ◽  
Screening Assays ◽  
On Chip

A Novel MEMS-Based Technique for In-Situ Characterization of Freestanding Nanometer Scale Thin Films Inside SEM and TEM

2001 ◽  
Author(s):  
M. A. Haque ◽  
M. T. A. Saif
Keyword(s):  
Thin Films ◽  
Tensile Testing ◽  
Uniaxial Tensile ◽  
Aluminum Specimen ◽  
Sem And Tem ◽  
Uniaxial Tensile Testing ◽  
Testing Facility ◽  
Film Specimen ◽  
On Chip

Abstract We present a MEMS-based technique for in-situ uniaxial tensile testing of freestanding thin films inside SEM and TEM. It integrates a freestanding thin film specimen with MEMS force sensors and structures to produce an on-chip tensile testing facility. Cofabrication of the specimen with force and displacement measuring mechanisms produces the following unique features: 1) Quantitative experimentation can be carried out in both SEM and TEM, 2) No extra gripping mechanism is required, 3) Specimen misalignment can be eliminated, 4) Pre-stress in specimen can be determined, and 5) Specimens with micrometer to nanometer thickness can be tested. We demonstrate the technique by testing a 200-nanometer thick Aluminum specimen in-situ in SEM. Significant strengthening and anelasticity were observed at this size scale.

An automated centrifugal microfluidic assay for whole blood fractionation and isolation of multiple cell populations using an aqueous two-phase system

Lab on a Chip ◽  
2021 ◽  
Author(s):  
Byeong-Ui Moon ◽  
Liviu Clime ◽  
Daniel Brassard ◽  
Alex Boutin ◽  
Jamal Daoud ◽  
...  
Keyword(s):  
Human Blood ◽  
Whole Blood ◽  
Cell Populations ◽  
Phase System ◽  
Two Phase ◽  
Microfluidic Platform ◽  
Aqueous Two Phase System ◽  
Separation Layers ◽  
Multiple Cell ◽  
On Chip

This paper describes an advanced on-chip whole human blood fractionation and cell isolation process combining an aqueous two-phase system to create complex separation layers with a centrifugal microfluidic platform to control and automate the assay.

scholarly journals Improved Procedure for Electroporation of Peptides into Adherent Cells In Situ

BioTechniques ◽  
1997 ◽  
Vol 23 (4) ◽  
pp. 644-646 ◽  
Author(s):  
Kevin L. Firth ◽  
Heather L. Brownell ◽  
Leda Raptis
Keyword(s):  
Adherent Cells
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