A nitroreductase and acidity detecting dual functional ratiometric fluorescent probe for selectively imaging tumor cells

2018 ◽  
Vol 16 (17) ◽  
pp. 3266-3272 ◽  
Author(s):  
Zhaoshuai He ◽  
Yajie Chou ◽  
Hanxin Zhou ◽  
Han Zhang ◽  
Tanyu Cheng ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
A549 Cells ◽  
Living Cells ◽  
Acidic Environment ◽  
Dual Functional ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Ratiometric Fluorescent Probe

A dual functional ratiometric fluorescent probe can obviously distinguish acidity, nitroreductase, and nitroreductase in an acidic environment. Confocal fluorescence imaging of A549 cells indicates the probe can detect acidity and expressed nitroreductase in living cells.

scholarly journals A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 411 ◽  
Author(s):  
Xiaohua Ma ◽  
Yuanqiang Hao ◽  
Jiaxiang Liu ◽  
Guoguang Wu ◽  
Lin Liu
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
A549 Cells ◽  
Living Cells ◽  
Fluorescence Signal ◽  
Sulfonate Group ◽  
Turn On ◽  
Highly Sensitive ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging

A new green-emitting fluorescent probe 1 was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe 1 and resulting inhibition of the probe’s photoinduced electron transfer (PET) process. Probe 1 exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold). Furthermore, confocal fluorescence imaging indicated that probe 1 was membrane-permeable and suitable for visualization of biothiols in living A549 cells.

scholarly journals A CycloRGDf(Me-V) Analog as Chemical Probe to Study Integrins Function in Living Cells

2020 ◽  
Author(s):  
Vojtech Kapras ◽  
Wyatt T. Slattery ◽  
Frederic Menard
Keyword(s):  
Flow Cytometry ◽  
Dissociation Constant ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Genetic Modification ◽  
Living Cells ◽  
Chemical Probe ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Cell Engulfment

<p>We report a chemical probe that can be used to image integrins in living cells. The fluorescent probe was derived from cyclo-RGDf(Me-V), a compound selective for integrins that possess an RGD-binding domain. We describe its synthesis and we demonstrate its use to detect integrin αVβ5 in cells. The probe’s dissociation constant for the integrin αVβ5 protein is 0.18 μM. The probe's activity was validated in murine BV-2 microglial cells using cell engulfment assays, flow cytometry, and confocal fluorescence imaging. This probe will provide access to spatiotemporally resolved studies of RGD-binding integrin function in living cells without the need for genetic modification.</p>

scholarly journals A CycloRGDf(Me-V) Analog as Chemical Probe to Study Integrins Function in Living Cells

2020 ◽  
Author(s):  
Vojtech Kapras ◽  
Wyatt T. Slattery ◽  
Frederic Menard
Keyword(s):  
Flow Cytometry ◽  
Dissociation Constant ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Genetic Modification ◽  
Living Cells ◽  
Chemical Probe ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Cell Engulfment

<p>We report a chemical probe that can be used to image integrins in living cells. The fluorescent probe was derived from cyclo-RGDf(Me-V), a compound selective for integrins that possess an RGD-binding domain. We describe its synthesis and we demonstrate its use to detect integrin αVβ5 in cells. The probe’s dissociation constant for the integrin αVβ5 protein is 0.18 μM. The probe's activity was validated in murine BV-2 microglial cells using cell engulfment assays, flow cytometry, and confocal fluorescence imaging. This probe will provide access to spatiotemporally resolved studies of RGD-binding integrin function in living cells without the need for genetic modification.</p>

A remarkable ratiometric fluorescent chemodosimeter for very rapid detection of hydrogen sulfide in the vapour phase and living cells

2015 ◽  
Vol 39 (11) ◽  
pp. 8940-8947 ◽  
Author(s):  
Sima Paul ◽  
Shyamaprosad Goswami ◽  
Chitrangada Das Mukhopadhyay
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Rapid Detection ◽  
Stokes Shift ◽  
Vapour Phase ◽  
Fast Response ◽  
Living Cells ◽  
Fluorescent Chemodosimeter ◽  
Ratiometric Fluorescent Probe

A ratiometric fluorescent probe having a fast response and a large Stokes shift detects SH− both in solid and vapour phases and this probe is used for fluorescence imaging of SH− in living cells.

Development of a FRET-based ratiometric fluorescent probe to monitor the changes in palladium(ii) in aqueous solution and living cells

2019 ◽  
Vol 43 (2) ◽  
pp. 552-555 ◽  
Author(s):  
Li Wang ◽  
Mingguang Ren ◽  
Zihong Li ◽  
Lixuan Dai ◽  
Weiying Lin
Keyword(s):  
Aqueous Solution ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Small Molecule ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

We have developed a new small-molecule based, mitochondrial-targeted ratiometric fluorescent palladium(ii) probe (CR-Pd). Fluorescence imaging shows that CR-Pd is suitable for the ratiometric visualization of palladium(ii) in living cells.

Construction of a novel ratiometric near-infrared fluorescent probe for SO2 derivatives and its application for biological imaging

2017 ◽  
Vol 9 (25) ◽  
pp. 3790-3794 ◽  
Author(s):  
Huiming Shang ◽  
Keyin Liu ◽  
Weiying Lin
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Living Cells ◽  
Biological Imaging ◽  
Ratiometric Fluorescent Probe

A novel near-infrared ratiometric fluorescent probe for detecting SO2 derivatives was developed and used for fluorescence imaging in living cells.

A lysosome-targeted and ratiometric fluorescent probe for imaging exogenous and endogenous hypochlorous acid in living cells

2016 ◽  
Vol 4 (27) ◽  
pp. 4739-4745 ◽  
Author(s):  
Mingguang Ren ◽  
Beibei Deng ◽  
Kai Zhou ◽  
Xiuqi Kong ◽  
Jian-Yong Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Small Molecule ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Ratiometric Fluorescent Probe

We have developed the first small-molecule based, lysosomal-targeted ratiometric fluorescent HClO probe (Lyso-HA). Fluorescence imaging shows that it is suitable for ratiometric visualization of exogenous and endogenous HClO at lysosomes in living cells.

Confocal Fluorescence Imaging of Photosensitised DNA Denaturation in Cell Nuclei

2005 ◽  
Author(s):  
Tytus Bernas ◽  
Elikplimi K. Asem ◽  
J. Paul Robinson ◽  
Peter R. Cook ◽  
Jurek W. Dobrucki
Keyword(s):  
Fluorescence Imaging ◽  
Dna Denaturation ◽  
Cell Nuclei ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging
Sign in / Sign up

Export Citation Format

Share Document