scholarly journals A CycloRGDf(Me-V) Analog as Chemical Probe to Study Integrins Function in Living Cells

2020 ◽  
Author(s):  
Vojtech Kapras ◽  
Wyatt T. Slattery ◽  
Frederic Menard
Keyword(s):  
Flow Cytometry ◽  
Dissociation Constant ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Genetic Modification ◽  
Living Cells ◽  
Chemical Probe ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Cell Engulfment

<p>We report a chemical probe that can be used to image integrins in living cells. The fluorescent probe was derived from cyclo-RGDf(Me-V), a compound selective for integrins that possess an RGD-binding domain. We describe its synthesis and we demonstrate its use to detect integrin αVβ5 in cells. The probe’s dissociation constant for the integrin αVβ5 protein is 0.18 μM. The probe's activity was validated in murine BV-2 microglial cells using cell engulfment assays, flow cytometry, and confocal fluorescence imaging. This probe will provide access to spatiotemporally resolved studies of RGD-binding integrin function in living cells without the need for genetic modification.</p>

scholarly journals A CycloRGDf(Me-V) Analog as Chemical Probe to Study Integrins Function in Living Cells

2020 ◽  
Author(s):  
Vojtech Kapras ◽  
Wyatt T. Slattery ◽  
Frederic Menard
Keyword(s):  
Flow Cytometry ◽  
Dissociation Constant ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Genetic Modification ◽  
Living Cells ◽  
Chemical Probe ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Cell Engulfment

<p>We report a chemical probe that can be used to image integrins in living cells. The fluorescent probe was derived from cyclo-RGDf(Me-V), a compound selective for integrins that possess an RGD-binding domain. We describe its synthesis and we demonstrate its use to detect integrin αVβ5 in cells. The probe’s dissociation constant for the integrin αVβ5 protein is 0.18 μM. The probe's activity was validated in murine BV-2 microglial cells using cell engulfment assays, flow cytometry, and confocal fluorescence imaging. This probe will provide access to spatiotemporally resolved studies of RGD-binding integrin function in living cells without the need for genetic modification.</p>

A nitroreductase and acidity detecting dual functional ratiometric fluorescent probe for selectively imaging tumor cells

2018 ◽  
Vol 16 (17) ◽  
pp. 3266-3272 ◽  
Author(s):  
Zhaoshuai He ◽  
Yajie Chou ◽  
Hanxin Zhou ◽  
Han Zhang ◽  
Tanyu Cheng ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
A549 Cells ◽  
Living Cells ◽  
Acidic Environment ◽  
Dual Functional ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging ◽  
Ratiometric Fluorescent Probe

A dual functional ratiometric fluorescent probe can obviously distinguish acidity, nitroreductase, and nitroreductase in an acidic environment. Confocal fluorescence imaging of A549 cells indicates the probe can detect acidity and expressed nitroreductase in living cells.

scholarly journals A Green-emitting Fluorescent Probe Based on a Benzothiazole Derivative for Imaging Biothiols in Living Cells

Molecules ◽  
2019 ◽  
Vol 24 (3) ◽  
pp. 411 ◽  
Author(s):  
Xiaohua Ma ◽  
Yuanqiang Hao ◽  
Jiaxiang Liu ◽  
Guoguang Wu ◽  
Lin Liu
Keyword(s):  
Fluorescent Probe ◽  
Stokes Shift ◽  
A549 Cells ◽  
Living Cells ◽  
Fluorescence Signal ◽  
Sulfonate Group ◽  
Turn On ◽  
Highly Sensitive ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging

A new green-emitting fluorescent probe 1 was developed for biothiol detection. The sensing mechanism was considered to be biothiol-induced cleavage of the 2,4-dinitrobenzene- sulfonate group in probe 1 and resulting inhibition of the probe’s photoinduced electron transfer (PET) process. Probe 1 exhibited favorable properties such as excellent selectivity, highly sensitive (0.12 µM), large Stokes shift (117 nm) and a remarkable turn-on fluorescence signal (148-fold). Furthermore, confocal fluorescence imaging indicated that probe 1 was membrane-permeable and suitable for visualization of biothiols in living A549 cells.

Confocal Fluorescence Imaging of Photosensitised DNA Denaturation in Cell Nuclei

2005 ◽  
Author(s):  
Tytus Bernas ◽  
Elikplimi K. Asem ◽  
J. Paul Robinson ◽  
Peter R. Cook ◽  
Jurek W. Dobrucki
Keyword(s):  
Fluorescence Imaging ◽  
Dna Denaturation ◽  
Cell Nuclei ◽  
Confocal Fluorescence ◽  
Confocal Fluorescence Imaging

A rhodamine-based fluorescent probe for Fe3+: synthesis, theoretical calculation and bioimaging application

2019 ◽  
Vol 11 (19) ◽  
pp. 2565-2570 ◽  
Author(s):  
Zhao Cheng ◽  
Lei Zheng ◽  
Hao Xu ◽  
Long Pang ◽  
Hao He
Keyword(s):  
Theoretical Calculation ◽  
Fluorescent Probe ◽  
Fluorescence Imaging ◽  
Living Cells ◽  
Low Cytotoxicity

A rhodamine-based fluorescent probe J1 with low cytotoxicity and characteristic fluorescence imaging of intracellular Fe3+ in living cells L929.

Pore selectivity and electron transfers in HZSM-5 single crystals: a Raman microspectroscopy mapping and confocal fluorescence imaging combined study

2020 ◽  
Vol 22 (22) ◽  
pp. 12745-12756
Author(s):  
A. Moissette ◽  
M. Hureau ◽  
M. Moreau ◽  
J. P. Cornard
Keyword(s):  
Single Crystals ◽  
Fluorescence Imaging ◽  
Single Particle ◽  
Raman Microspectroscopy ◽  
Single Particle Level ◽  
Confocal Fluorescence ◽  
Particle Level ◽  
Confocal Fluorescence Imaging ◽  
Electron Transfers

Electron transfers at the single particle level in HZSM-5 zeolite are followed by combining Raman microspectroscopy mapping and confocal fluorescence imaging. The effects of pore accessibility and guest diffusion on reactivity are investigated.

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