scholarly journals One-step synthesis of amino-functionalized up-converting NaYF4:Yb,Er nanoparticles for in vitro cell imaging

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27429-27437 ◽  
Author(s):  
Lidija Mancic ◽  
Aleksandra Djukic-Vukovic ◽  
Ivana Dinic ◽  
Marko G. Nikolic ◽  
Mihailo D. Rabasovic ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Single Molecule ◽  
Cell Imaging ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Single Molecule Tracking ◽  
Wide Range ◽  
Deep Tissue Imaging ◽  
One Step

The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery.

One-Step Synthesis of Small-Sized and Water-Soluble NaREF4Upconversion Nanoparticles for In Vitro Cell Imaging and Drug Delivery

2013 ◽  
Vol 19 (8) ◽  
pp. 2685-2694 ◽  
Author(s):  
Dongmei Yang ◽  
Yunlu Dai ◽  
Pingan Ma ◽  
Xiaojiao Kang ◽  
Ziyong Cheng ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Imaging ◽  
Water Soluble ◽  
One Step

scholarly journals Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging

2018 ◽  
Vol 5 (3) ◽  
pp. 171614 ◽  
Author(s):  
M. Vijaya Bharathi ◽  
Santanu Maiti ◽  
Bidisha Sarkar ◽  
Kaustab Ghosh ◽  
Priyankar Paira
Keyword(s):  
Quantum Dot ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Water Soluble ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Non Invasive ◽  
Green Approach ◽  
Deep Tissue Imaging ◽  
Bio Imaging

This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs were further modified with streptavidin and then bound to biotin because of its high conjugation efficiency. Labelling and bio-imaging of cells with these bio-conjugated QDs were evaluated. The bright red fluorescence from these types of QDs in HeLa cells makes these materials suitable for deep tissue imaging.

Ablation of Red Stable Transfected Prostate Cancer Cell Lines by C-CPE Gold-Nanoparticle Mediated Laser Intervention

2021 ◽  
Author(s):  
Suhayla Alnajjar ◽  
Ingo Nolte ◽  
Annegret Becker ◽  
Tina Kostka ◽  
Jan Torben Schille ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Gold Nanoparticle ◽  
Cell Lines ◽  
Cancer Cell ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging

Abstract Background: Claudin (CLDN) proteins have been described to be found and accordingly targeted to evaluate novel therapeutic approaches. C-terminus of Clostridium perfringens enterotoxin (C-CPE) binds efficiently several claudins and thus recombinant C-CPE conjugated to gold nanoparticles (AuNPs) has been used for cancer cell targeting using gold nanoparticle- mediated laser perforation (GNOME-LP). Cancer cells inoculation is routinely used to generate in vivo models to evaluate novel therapeutic approaches in prostate cancer. However, detailed characterization of cancer spreading and early tumor development and therapeutic response is often limited as conventional cell lines do not allow advanced deep tissue imaging.Methods: two canine prostate cancer cell lines were stably transfected with red fluorescent protein (RFP), followed by G418 selection. RFP marker as well as CLDN3, -4 and -7 expression was comparatively confirmed by flow cytometry, qPCR and immunofluorescences. For cancer cells targeting, GNOME-LP at a laser fluence of 72 mJ/cm² and a scanning speed of 0.5 cm/s was used. Statistical analysis was performed using SAS software 7.1, Dunnett´s Multiple Comparison Test and Student´s two-sided t-test. Differences were considered statistically significant for p<0.05.Results: we established two canine prostate carcinoma cell lines, stably expressing RFP allowing perspective deep tissue imaging. Directed C-CPE-AuNP binding to native and RFP transfected cells verified the capability to specifically target CLDN receptors. Cancer cell ablation was demonstrated in vitro setting using a combination of gold nanoparticle mediated laser perforation and C-CPE-AuNPs treatment reducing tumor cell viability to less than 10 % depending on cell line. Conclusion: the results confirm that this therapeutic approach can be used efficiently to target prostate carcinoma cells carrying a marker protein allowing deep tissue imaging. The established cell lines and the verified proof of concept in vitro study provide the basis for perspective Xenograft model in vivo studies. The introduce red fluorescence enables deep tissue imaging in living animals and therefore detailed characterization of tumor growth and subsequently possible tumor ablation through C-CPE-AuNPs treatment.

Responsive optical probes for deep-tissue imaging: Photoacoustics and second near-infrared fluorescence

2021 ◽  
Vol 173 ◽  
pp. 141-163
Author(s):  
Fei Ding ◽  
Jing Feng ◽  
Xueli Zhang ◽  
Jielin Sun ◽  
Chunhai Fan ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Optical Probes ◽  
Near Infrared Fluorescence ◽  
Deep Tissue Imaging

scholarly journals Slow light for deep tissue imaging with ultrasound modulation

2012 ◽  
Vol 100 (13) ◽  
pp. 131102 ◽  
Author(s):  
Huiliang Zhang ◽  
Mahmood Sabooni ◽  
Lars Rippe ◽  
Chulhong Kim ◽  
Stefan Kröll ◽  
...  
Keyword(s):  
Slow Light ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging ◽  
Ultrasound Modulation

scholarly journals Transgenic mice: beyond the knockout

2011 ◽  
Vol 300 (2) ◽  
pp. F291-F300 ◽  
Author(s):  
R. Lance Miller
Keyword(s):  
Transgenic Mice ◽  
Fluorescent Proteins ◽  
Primary Cultures ◽  
Cell Lineage ◽  
Tissue Imaging ◽  
Cell Type ◽  
Deep Tissue ◽  
Pure Cell ◽  
Deep Tissue Imaging ◽  
Tremendous Impact

Transgenic mice have had a tremendous impact on biomedical research. Most researchers are familiar with transgenic mice that carry Cre recombinase (Cre) and how they are used to create conditional knockouts. However, some researchers are less familiar with many of the other types of transgenic mice and their applications. For example, transgenic mice can be used to study biochemical and molecular pathways in primary cultures and cell suspensions derived from transgenic mice, cell-cell interactions using multiple fluorescent proteins in the same mouse, and the cell cycle in real time and in the whole animal, and they can be used to perform deep tissue imaging in the whole animal, follow cell lineage during development and disease, and isolate large quantities of a pure cell type directly from organs. These novel transgenic mice and their applications provide the means for studying of molecular and biochemical events in the whole animal that was previously limited to cell cultures. In conclusion, transgenic mice are not just for generating knockouts.

Designing Upconversion Nanocrystals Capable of 745 nm Sensitization and 803 nm Emission for Deep-Tissue Imaging

2016 ◽  
Vol 22 (31) ◽  
pp. 10801-10807 ◽  
Author(s):  
Liangliang Liang ◽  
Xiaoji Xie ◽  
Daniel Teh Boon Loong ◽  
Angelo Homayoun All ◽  
Ling Huang ◽  
...  
Keyword(s):  
Tissue Imaging ◽  
Deep Tissue ◽  
Upconversion Nanocrystals ◽  
Deep Tissue Imaging

scholarly journals Near-infrared probe-based confocal microendoscope for deep-tissue imaging

2018 ◽  
Vol 9 (10) ◽  
pp. 5011 ◽  
Author(s):  
Jiafu Wang ◽  
Hua Li ◽  
Geng Tian ◽  
Yong Deng ◽  
Qian Liu ◽  
...  
Keyword(s):  
Near Infrared ◽  
Tissue Imaging ◽  
Deep Tissue ◽  
Deep Tissue Imaging
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