scholarly journals Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging

2018 ◽  
Vol 5 (3) ◽  
pp. 171614 ◽  
Author(s):  
M. Vijaya Bharathi ◽  
Santanu Maiti ◽  
Bidisha Sarkar ◽  
Kaustab Ghosh ◽  
Priyankar Paira

This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs were further modified with streptavidin and then bound to biotin because of its high conjugation efficiency. Labelling and bio-imaging of cells with these bio-conjugated QDs were evaluated. The bright red fluorescence from these types of QDs in HeLa cells makes these materials suitable for deep tissue imaging.

2020 ◽  
Vol 93 (10) ◽  
pp. 1226-1233
Author(s):  
Shozo Onishi ◽  
Yasutaka Suzuki ◽  
Hikari Ano ◽  
Jun Kawamata

2017 ◽  
Vol 8 (11) ◽  
pp. 7696-7704 ◽  
Author(s):  
Yong Woong Jun ◽  
Hye Rim Kim ◽  
Ye Jin Reo ◽  
Mingchong Dai ◽  
Kyo Han Ahn

We have developed a new class of two-photon absorbing dyes that are far-red emitting, water-soluble, and very bright inside cells as well as in tissue. The significant autofluorescence from yellow wavelength region in tissue imaging can be addressed by deep-red emitting dyes.


2015 ◽  
Vol 20 ◽  
pp. 94-103 ◽  
Author(s):  
Timothy R. Olsen ◽  
Lundy L. Davis ◽  
Samantha E. Nicolau ◽  
Caroline C. Duncan ◽  
Daniel C. Whitehead ◽  
...  

RSC Advances ◽  
2018 ◽  
Vol 8 (48) ◽  
pp. 27429-27437 ◽  
Author(s):  
Lidija Mancic ◽  
Aleksandra Djukic-Vukovic ◽  
Ivana Dinic ◽  
Marko G. Nikolic ◽  
Mihailo D. Rabasovic ◽  
...  

The emerging up-conversion nanoparticles (UCNPs) offer a wide range of biotechnology applications, from biomarkers and deep tissue imaging, to single molecule tracking and drug delivery.


2021 ◽  
Vol 173 ◽  
pp. 141-163
Author(s):  
Fei Ding ◽  
Jing Feng ◽  
Xueli Zhang ◽  
Jielin Sun ◽  
Chunhai Fan ◽  
...  

2012 ◽  
Vol 100 (13) ◽  
pp. 131102 ◽  
Author(s):  
Huiliang Zhang ◽  
Mahmood Sabooni ◽  
Lars Rippe ◽  
Chulhong Kim ◽  
Stefan Kröll ◽  
...  

2011 ◽  
Vol 300 (2) ◽  
pp. F291-F300 ◽  
Author(s):  
R. Lance Miller

Transgenic mice have had a tremendous impact on biomedical research. Most researchers are familiar with transgenic mice that carry Cre recombinase (Cre) and how they are used to create conditional knockouts. However, some researchers are less familiar with many of the other types of transgenic mice and their applications. For example, transgenic mice can be used to study biochemical and molecular pathways in primary cultures and cell suspensions derived from transgenic mice, cell-cell interactions using multiple fluorescent proteins in the same mouse, and the cell cycle in real time and in the whole animal, and they can be used to perform deep tissue imaging in the whole animal, follow cell lineage during development and disease, and isolate large quantities of a pure cell type directly from organs. These novel transgenic mice and their applications provide the means for studying of molecular and biochemical events in the whole animal that was previously limited to cell cultures. In conclusion, transgenic mice are not just for generating knockouts.


2016 ◽  
Vol 22 (31) ◽  
pp. 10801-10807 ◽  
Author(s):  
Liangliang Liang ◽  
Xiaoji Xie ◽  
Daniel Teh Boon Loong ◽  
Angelo Homayoun All ◽  
Ling Huang ◽  
...  

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