scholarly journals Ultrasensitive magnetic resonance imaging of systemic reactive oxygen species in vivo for early diagnosis of sepsis using activatable nanoprobes

2019 ◽  
Vol 10 (13) ◽  
pp. 3770-3778 ◽  
Author(s):  
Huan Wang ◽  
Dongqin Yu ◽  
Bo Li ◽  
Zhen Liu ◽  
Jinsong Ren ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Reactive Oxygen Species ◽  
Magnetic Resonance ◽  
Early Diagnosis ◽  
Contrast Agents ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Resonance Imaging

Novel ROS-activated contrast agents are designed for magnetic resonance imaging of ROS for early diagnosis of sepsis.

scholarly journals Reactive Oxygen Species Generation in Human Cells by a Novel Magnetic Resonance Imaging Contrast Agent

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Li Wang ◽  
Eric Lin ◽  
Mary J. Johansen ◽  
Timothy Madden ◽  
Edward Felix ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Reactive Oxygen Species ◽  
Aqueous Solution ◽  
Magnetic Resonance ◽  
Cultured Cells ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Ros Production ◽  
Oxygen Species ◽  
Resonance Imaging

The novel positive-contrast magnetic resonance imaging (MRI) marker C4 consists of an aqueous solution of cobalt chloride (CoCl2) complexed with the chelator N-acetylcysteine (NAC). We evaluated whether the presence of C4 or its components would produce reactive oxygen species (ROS, including hydroxyl, peroxyl, or other reactive oxygen species) in cultured cells. Human cancer or normal cells were incubated with 1% (w/v) CoCl2·6H2O or 2% NAC or a combination of both (1% CoCl2·6H2O : 2% NAC in an aqueous solution, abbreviated as Co : NAC) in the presence or absence of H2O2. Intracellular ROS levels were measured and quantified by change in relative fluorescence units. Student’s t-tests were used. In all cell lines exposed to 1000 μM H2O2, the Co : NAC led to ≥94.7% suppression of ROS at 5 minutes and completely suppressed ROS at 60 and 90 minutes; NAC suppressed ROS by ≥76.6% at 5 minutes and by ≥94.5% at 90 minutes; and CoCl2·6H2O suppressed ROS by ≥37.2% at 30 minutes and by ≥48.6% at 90 minutes. These results demonstrate that neither Co : NAC nor its components generated ROS; rather, they suppressed ROS production in cultured cells, suggesting that C4 would not enhance ROS production in clinical use.

Supramolecular Enhancement of Aminoxyl ORCA Contrast Agents

2019 ◽  
Author(s):  
Hamilton Lee ◽  
Jenica Lumata ◽  
Michael A. Luzuriaga ◽  
Candace Benjamin ◽  
Olivia Brohlin ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Side Effects ◽  
Magnetic Resonance ◽  
Tobacco Mosaic Virus ◽  
Contrast Agents ◽  
Single Molecule ◽  
Organic Radical ◽  
Resonance Imaging ◽  
Physiological Conditions

<div><div><div><p>Many contrast agents for magnetic resonance imaging are based on gadolinium, however side effects limit their use in some patients. Organic radical contrast agents (ORCAs) are potential alternatives, but are reduced rapidly in physiological conditions and have low relaxivities as single molecule contrast agents. Herein, we use a supramolecular strategy where cucurbit[8]uril binds with nanomolar affinities to ORCAs and protects them against biological reductants to create a stable radical in vivo. We further over came the weak contrast by conjugating this complex on the surface of a self-assembled biomacromolecule derived from the tobacco mosaic virus.</p></div></div></div>

scholarly journals Uniform Fe3O4/Gd2O3-DHCA nanocubes for dual-mode magnetic resonance imaging

2020 ◽  
Vol 11 ◽  
pp. 1000-1009
Author(s):  
Miao Qin ◽  
Yueyou Peng ◽  
Mengjie Xu ◽  
Hui Yan ◽  
Yizhu Cheng ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
Mri Contrast Agents ◽  
Clinical Diagnostics ◽  
Resonance Imaging ◽  
Dual Mode ◽  
Mri Contrast ◽  
Magnetic Resonance Imaging Mri

The multimodal magnetic resonance imaging (MRI) technique has been extensively studied over the past few years since it offers complementary information that can increase diagnostic accuracy. Simple methods to synthesize contrast agents are necessary for the development of multimodal MRI. Herein, uniformly distributed Fe3O4/Gd2O3 nanocubes for T 1–T 2 dual-mode MRI contrast agents were successfully designed and synthesized. In order to increase hydrophilicity and biocompatibility, the nanocubes were coated with nontoxic 3,4-dihydroxyhydrocinnamic acid (DHCA). The results show that iron (Fe) and gadolinium (Gd) were homogeneously distributed throughout the Fe3O4/Gd2O3-DHCA (FGDA) nanocubes. Relaxation time analysis was performed on the images obtained from the 3.0 T scanner. The results demonstrated that r 1 and r 2 maximum values were 67.57 ± 6.2 and 24.2 ± 1.46 mM−1·s−1, respectively. In vivo T 1- and T 2-weighted images showed that FGDA nanocubes act as a dual-mode contrast agent enhancing MRI quality. Overall, these experimental results suggest that the FGDA nanocubes are interesting tools that can be used to increase MRI quality, enabling accurate clinical diagnostics.

Bio-interfacial magnetic resonance imaging of hyperpolarized contrast agents for metabolic flux interrogation in vivo

2016 ◽  
Vol 36 ◽  
pp. 224-228 ◽  
Author(s):  
Gijin Jeong ◽  
Jee-Wook Lee ◽  
Gyeonghyeon Gong ◽  
Hyeji Ko ◽  
Ikjang Choi ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
Metabolic Flux ◽  
Resonance Imaging

scholarly journals Fluorine-19 Mri Contrast Agents for Cell Tracking and Lung Imaging

2015 ◽  
Vol 8s1 ◽  
pp. MRI.S23559 ◽  
Author(s):  
Matthew S. Fox ◽  
Jeffrey M. Gaudet ◽  
Paula J. Foster
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
Cell Tracking ◽  
Cellular Therapy ◽  
Medical Intervention ◽  
Mri Contrast Agents ◽  
Lung Imaging ◽  
Resonance Imaging

Fluorine-19 (19F)-based contrast agents for magnetic resonance imaging stand to revolutionize imaging-based research and clinical trials in several fields of medical intervention. First, their use in characterizing in vivo cell behavior may help bring cellular therapy closer to clinical acceptance. Second, their use in lung imaging provides novel noninvasive interrogation of the ventilated airspaces without the need for complicated, hard-to-distribute hardware. This article reviews the current state of 19F-based cell tracking and lung imaging using magnetic resonance imaging and describes the link between the methods across these fields and how they may mutually benefit from solutions to mutual problems encountered when imaging 19F-containing compounds, as well as hardware and software advancements.

scholarly journals Emerging Applications of Porphyrins and Metalloporphyrins in Biomedicine and Diagnostic Magnetic Resonance Imaging

Biosensors ◽  
2018 ◽  
Vol 8 (4) ◽  
pp. 95 ◽  
Author(s):  
Muhammad Imran ◽  
Muhammad Ramzan ◽  
Ahmad Qureshi ◽  
Muhammad Khan ◽  
Muhammad Tariq
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
Photophysical Properties ◽  
Biomedical Science ◽  
Resonance Imaging ◽  
Structural Modifications ◽  
Bio Imaging ◽  
Magnetic Resonance Imaging Mri

In recent years, scientific advancements have constantly increased at a significant rate in the field of biomedical science. Keeping this in view, the application of porphyrins and metalloporphyrins in the field of biomedical science is gaining substantial importance. Porphyrins are the most widely studied tetrapyrrole-based compounds because of their important roles in vital biological processes. The cavity of porphyrins containing four pyrrolic nitrogens is well suited for the binding majority of metal ions to form metalloporphyrins. Porphyrins and metalloporphyrins possess peculiar photochemical, photophysical, and photoredox properties which are tunable through structural modifications. Their beneficial photophysical properties, such as the long wavelength of emission and absorption, high singlet oxygen quantum yield, and low in vivo toxicity, have drawn scientists’ interest to discover new dimensions in the biomedical field. Applications of porphyrins and metalloporphyrins have been pursued in the perspective of contrast agents for magnetic resonance imaging (MRI), photodynamic therapy (PDT) of cancer, bio-imaging, and other biomedical applications. This review discusses photophysics and the photochemistry of porphyrins and their metal complexes. Secondly, it explains the current developments and mode of action for contrast agents for MRI. Moreover, the application of porphyrin and metalloporphyrin-based molecules as a photosensitizer in PDT of cancer, the mechanism of the generation of reactive oxygen species (ROS), factors that determine the efficiency of PDT, and the developments to improve this technology are delineated. The last part explores the most recent research and developments on metalloporphyrin-based materials in bio-imaging, drug delivery, and the determination of ferrochelatase in bone marrow indicating their prospective clinical applications.

Holmium phosphate nanoparticles as negative contrast agents for high-field magnetic resonance imaging: Synthesis, magnetic relaxivity study and in vivo evaluation

2021 ◽  
Vol 587 ◽  
pp. 131-140
Author(s):  
Elisabet Gómez-González ◽  
Carlos Caro ◽  
Diego Martínez-Gutiérrez ◽  
María L. García-Martín ◽  
Manuel Ocaña ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agents ◽  
High Field ◽  
Negative Contrast ◽  
Resonance Imaging ◽  
In Vivo Evaluation
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