scholarly journals Assembly of FN-silk with laminin-521 to integrate hPSCs into a three-dimensional culture for neural differentiation

2020 ◽  
Vol 8 (9) ◽  
pp. 2514-2525
Author(s):  
Carolina Åstrand ◽  
Veronique Chotteau ◽  
Anna Falk ◽  
My Hedhammar
Keyword(s):  
Culture System ◽  
Neural Differentiation ◽  
Spider Silk ◽  
Three Dimensional ◽  
3D Culture ◽  
Silk Protein ◽  
Spider Silk Protein ◽  
3D Culture System ◽  
Three Dimensional Culture

The functionalized recombinant spider silk protein FN-silk can self-assemble into a 3D microfiber network. When combined with recombinant laminin521 it provides a 3D culture system suitable for expansion of hPSCs and following neural differentiation.

Novel three-dimensional long-term bone marrow culture system using polymer particles with grafted epoxy-polymer-chains supports the proliferation and differentiation of hematopoietic stem cells

2011 ◽  
Vol 236 (11) ◽  
pp. 1342-1350 ◽  
Author(s):  
Yukio Hirabayashi ◽  
Yoshihiro Hatta ◽  
Jin Takeuchi ◽  
Isao Tsuboi ◽  
Tomonori Harada ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Culture System ◽  
3D Structure ◽  
Three Dimensional ◽  
Hematopoietic Cells ◽  
3D Culture ◽  
Polymer Chains ◽  
Polymer Particles ◽  
Hematopoietic Stem ◽  
3D Culture System

Hematopoiesis occurs in the bone marrow, where primitive hematopoietic cells proliferate and differentiate in close association with a three-dimensional (3D) hematopoietic microenvironment composed of stromal cells. We examined the hematopoietic supportive ability of stromal cells in a 3D culture system using polymer particles with grafted epoxy polymer chains. Umbilical cord blood-derived CD34+ cells were co-cultivated with MS-5 stromal cells. They formed a 3D structure in the culture dish in the presence of particles, and the total numbers of cells and the numbers of hematopoietic progenitor cells, including colony-forming unit (CFU)-Mix, CFU-granulocyte-macrophage, CFU-megakaryocyte and burst-forming unit-erythroid, were measured every seven days. The hematopoietic supportive activity of the 3D culture containing polymer particles and stromal cells was superior to that of 2D culture, and allowed the expansion and maintenance of hematopoietic progenitor cells for more than 12 weeks. Various types of hematopoietic cells, including granulocytes, macrophages and megakaryocytes at different maturation stages, appeared in the 3D culture, suggesting that the CD34+ cells were able to differentiate into a range of blood cell types. Morphological examination showed that MS-5 stromal cells grew on the surface of the particles and bridged the gaps between them to form a 3D structure. Hematopoietic cells slipped into the 3D layer and proliferated within it, relying on the presence of the MS-5 cells. These results suggest that this 3D culture system using polymer particles reproduced the hematopoietic phenomenon in vitro, and might thus provide a new tool for investigating hematopoietic stem cell–stromal cell interactions.

A novel in vitro model of human implantation: an endometrium-like three-dimensional (3D) culture system for attachment/invasion of trophoblast-like cells

2010 ◽  
Vol 94 (4) ◽  
pp. S64-S65
Author(s):  
H. Wang ◽  
F. Pilla ◽  
S. Martinez-Escribano ◽  
S. Bocca ◽  
S. Oehninger ◽  
...  
Keyword(s):  
Culture System ◽  
In Vitro Model ◽  
Three Dimensional ◽  
3D Culture ◽  
3D Culture System ◽  
Vitro Model ◽  
Human Implantation

scholarly journals The Three-Dimensional Culture System with Matrigel and Neurotrophic Factors Preserves the Structure and Function of Spiral Ganglion NeuronIn Vitro

2016 ◽  
Vol 2016 ◽  
pp. 1-15 ◽  
Author(s):  
Gaoying Sun ◽  
Wenwen Liu ◽  
Zhaomin Fan ◽  
Daogong Zhang ◽  
Yuechen Han ◽  
...  
Keyword(s):  
Growth Cone ◽  
Neurotrophic Factors ◽  
Culture System ◽  
Spiral Ganglion ◽  
Three Dimensional ◽  
3D Culture ◽  
Explant Culture ◽  
Structure And Function ◽  
3D Culture System ◽  
And Function

Whole organ culture of the spiral ganglion region is a resourceful model system facilitating manipulation and analysis of live sprial ganglion neurons (SGNs). Three-dimensional (3D) cultures have been demonstrated to have many biomedical applications, but the effect of 3D culture in maintaining the SGNs structure and function in explant culture remains uninvestigated. In this study, we used the matrigel to encapsulate the spiral ganglion region isolated from neonatal mice. First, we optimized the matrigel concentration for the 3D culture system and found the 3D culture system protected the SGNs against apoptosis, preserved the structure of spiral ganglion region, and promoted the sprouting and outgrowth of SGNs neurites. Next, we found the 3D culture system promoted growth cone growth as evidenced by a higher average number and a longer average length of filopodia and a larger growth cone area. 3D culture system also significantly elevated the synapse density of SGNs. Last, we found that the 3D culture system combined with neurotrophic factors had accumulated effects in promoting the neurites outgrowth compared with 3D culture or NFs treatment only groups. Together, we conclude that the 3D culture system preserves the structure and function of SGN in explant culture.

Maintenance and Expansion of Hematopoietic Stem/Progenitor Cells in a Biomimetic Hematopoietic Cells Niche.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 4115-4115
Author(s):  
Ting Liu ◽  
Jing Tan ◽  
Li Hou ◽  
Wentong Meng ◽  
Yuchun Wang
Keyword(s):  
Progenitor Cells ◽  
Culture System ◽  
Three Dimensional ◽  
3D Culture ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem ◽  
Cd34 Cells ◽  
3D Culture System ◽  
2D System

Abstract As main hematopoietic organ, bone marrow have three dimensional microenvironment for hematopoietic stem/progenitor cells grow in, so call “hematopoietic cells niche”, which are composed by stromal cells and extracelluar matrix. The interactions of cell to cell and cell to matrix between stem/progenitor cells with hematopoietic niche are facilitated by its three dimensional conformation. The biology behaviors of hematopoietic stem cells are mediate by many signal transductions between stem/progenitor cells with their corresponding microenvironment. Now there are strong evidence from animal model study suggests that osteoblasts might play an essential role in creation of a hematopoietic stem cell niche and thereby regulation of stem cell maintenance, proliferation, and maturation. In light of the structure-function relationship of bone marrow topography, we conceived a biomimetic culture system (3D culture system) with bio-derived bone as framework, composited with human marrow mesenchymal stem cells, and induced the cells into osteoblasts to simulate the effects of hematopoietic osteobalst niche. CD 34+ cells or mononuclear cells separated from umbilical cord blood were cultured for 2∼5 weeks in the 3D culture system and also in a conventional 2D culture system as control without additional cytokine supplement. Based on our results, higher expression of extracelluar matrix and N-cadherin were observed in 3D culture system compared to 2D system. At 2 weeks culture, 3D culture system showed higher number of CD34+ cells and CD34+/CD38- cells when compared with the input (P<0.05), the increased cells were predominant CD34+/CD38-cells. Although CD34+ cells were decreased at 5 weeks culture; nevertheless, CD34+/CD38- cells were still maintained at high level. We also observed that imbedding MNCs with a higher percentage of CD34+/CD38-cells cultured in 3D system (P<0.05), which may represent a down regulation of CD38 phenotype during culture. The function of cultured cells was evaluated in colony forming unit (CFU) assay and long term culture initial cell (LTC-IC) assay. 3D system produced higher expansion of CFU progenitors than 2D system (7.13–8.89 times vs. 1.22–1.31times) after 2 weeks culture. Of note, the colony distribution of 3D system manifested higher percentage of BFU-E and CFU-GEMM, while 2D system showed mainly CFU-GM. LTC-IC represents the primitive progenitor, 3D system showed a 6.2 times increment over input after 2 weeks culture. Furthermore, it was competent to maintain the immaturation of hematopoietic progenitor cells (HPCs) over 5 weeks. This study demonstrated that our 3D culture system constructed with the bio-derived bone composited with induced osteoblast is capable to allow maintenance and expansion of primitive hematopoietic progenitor cells in vitro. It may open a new avenue to study HSCs/HPCs behaviors and to achieve sustained primitive progenitor cell expansion.

scholarly journals Three-Dimensional Cell Culture Based on Magnetic Fields to Assemble Low-Grade Ovarian Carcinoma Cell Aggregates Containing Lymphocytes

Cells ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 635
Author(s):  
Caroline Natânia de Souza-Araújo ◽  
Cláudia Rodrigues Tonetti ◽  
Marcella Regina Cardoso ◽  
Liliana Aparecida Lucci de Angelo Andrade ◽  
Rodrigo Fernandes da Silva ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Cell Line ◽  
Culture System ◽  
Carcinoma Cell ◽  
Three Dimensional ◽  
3D Culture ◽  
Low Grade ◽  
Cell Aggregates ◽  
Cultured Cell ◽  
3D Culture System

There is a limited number of established ovarian cancer cell lines matching the low-grade serous histotype available for research purposes. Three-dimensional (3D) culture systems provide in vitro models with better tissue-like characteristics than two-dimensional (2D) systems. The goal in the study was to characterize the growth of a given low-grade serous ovarian carcinoma cell line in a 3D culture system conducted in a magnetic field. Moreover, the culture system was evaluated in respect to the assembly of malignant cell aggregates containing lymphocytes. CAISMOV24 cell line alone or mixed with human peripheral blood mononuclear cells (PBMC) were cultured using a commercially available 3D culture system designed for 24 well plates. Resulting cell aggregates revealed the intrinsic capacity of CAISMOV24 cells to assemble structures morphologically defined as papillary, and reflected molecular characteristics usually found in ovarian carcinomas. The contents of lymphocytes into co-cultured cell aggregates were significantly higher (p < 0.05) when NanoShuttle-conjugated PBMC were employed compared with non-conjugated PBMC. Moreover, lymphocyte subsets NK, T-CD4, T-CD8 and T-regulatory were successfully retrieved from co-cultured cell aggregates at 72h. Thus, the culture system allowed CAISMOV24 cell line to develop papillary-like cell aggregates containing lymphocytes.

CFD-Aided Design of a Dynamic Culture System for the Co-Culture of Adherent and Non-Adherent Cells

2009 ◽  
Author(s):  
Marco Cantini ◽  
Gianfranco B. Fiore ◽  
Alberto Redaelli ◽  
Monica Soncini
Keyword(s):  
Culture System ◽  
Three Dimensional ◽  
3D Culture ◽  
Packed Bed ◽  
High Dose Chemotherapy ◽  
Haematopoietic Stem Cell ◽  
High Dose ◽  
3D Culture System ◽  
In Vitro Expansion

Haematopoietic stem cell (HSC) transplantation has been widely used to treat patients that have undergone high-dose chemotherapy or radiotherapy for haematological or non-haematological malignancies, and is currently investigated for the treatment of several other pathologic conditions. Nevertheless, present and expected clinical applications are hindered by the shortage of cells available for transplantation. Hence, many researchers have attempted to achieve an in vitro expansion of HSCs, using different experimental set-ups and approaches, which range from traditional static monolayer cultures to three-dimensional (3D) dynamic systems. Specifically, several bioreactor systems have been proposed, including perfusion chambers, stirred, rotating, hollow fiber, and packed bed reactors [1]. Taken together, literature studies suggest that a dynamic 3D culture system may provide superior expansion of HSCs.

Three-dimensional scaffold made from recombinant spider silk protein for tissue engineering

2009 ◽  
Vol 426 (1) ◽  
pp. 127-130 ◽  
Author(s):  
I. I. Agapov ◽  
O. L. Pustovalova ◽  
M. M. Moisenovich ◽  
V. G. Bogush ◽  
O. S. Sokolova ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Spider Silk ◽  
Three Dimensional ◽  
Silk Protein ◽  
Spider Silk Protein
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