scholarly journals Quantification of micropolarity and microviscosity of aggregation and salt-induced gelation of sodium deoxycholate (NaDC) using Nile red fluorescence

2019 ◽  
Vol 18 (11) ◽  
pp. 2773-2781 ◽  
Author(s):  
Jitendriya Swain ◽  
Jhili Mishra ◽  
Goutam Ghosh ◽  
Ashok Kumar Mishra
Keyword(s):  
Nile Red ◽  
Sodium Deoxycholate ◽  
Fluorescence Properties ◽  
Red Fluorescence ◽  
Nile Red Fluorescence

Nile red fluorescence properties can be used for the estimation of micropolarity and microviscosity of the gel medium.

Cationic Liposomes in Mixed Didodecyldimethylammonium Bromide and Dioctadecyldimethylammonium Bromide Aqueous Dispersions Studied by Differential Scanning Calorimetry, Nile Red Fluorescence, and Turbidity

Langmuir ◽  
2006 ◽  
Vol 22 (8) ◽  
pp. 3579-3585 ◽  
Author(s):  
Eloi Feitosa ◽  
Fernanda Rosa Alves ◽  
Anna Niemiec ◽  
M. Elisabete C. D. Real Oliveira ◽  
Elisabete M. S. Castanheira ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Nile Red ◽  
Cationic Liposomes ◽  
Scanning Calorimetry ◽  
Aqueous Dispersions ◽  
Red Fluorescence ◽  
Didodecyldimethylammonium Bromide ◽  
Nile Red Fluorescence ◽  
Dioctadecyldimethylammonium Bromide

Rapid screening method for lipid production in alga based on Nile red fluorescence

2009 ◽  
Vol 33 (10) ◽  
pp. 1386-1392 ◽  
Author(s):  
Guan-Hua Huang ◽  
Gu Chen ◽  
Feng Chen
Keyword(s):  
Lipid Production ◽  
Screening Method ◽  
Nile Red ◽  
Rapid Screening ◽  
Red Fluorescence ◽  
Rapid Screening Method ◽  
Nile Red Fluorescence

Algal Lipid Quantification in-situ with Nile Red Fluorescence

2012 ◽  
Author(s):  
Yufeng Ge ◽  
J Alex Thomasson ◽  
Matt Korte
Keyword(s):  
Nile Red ◽  
Red Fluorescence ◽  
Lipid Quantification ◽  
Algal Lipid ◽  
Nile Red Fluorescence

Characteristic features of nile red fluorescence in transparent xerogels

2015 ◽  
Vol 49 (4) ◽  
pp. 249-254 ◽  
Author(s):  
M. S. Pilipenko ◽  
A. V. Koshkin ◽  
V. A. Sazhnikov ◽  
M. V. Alfimov
Keyword(s):  
Nile Red ◽  
Red Fluorescence ◽  
Nile Red Fluorescence ◽  
Characteristic Features

scholarly journals Nile Red Staining for Oil Determination in Microalgal Cells: A New Insight through Statistical Modelling

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Ronald Halim ◽  
Paul A. Webley
Keyword(s):  
Fluorescence Intensity ◽  
Nile Red ◽  
Biofuel Production ◽  
Staining Procedure ◽  
Surface Model ◽  
Second Phase ◽  
Red Fluorescence ◽  
Nile Red Fluorescence ◽  
Red Dye ◽  
Nile Red Staining

In the wake of global warming and rapid fossil fuel depletion, microalgae emerge as promising feedstocks for sustainable biofuel production. Nile red staining acts as a rapid diagnostic tool to measure the amount of biodiesel-convertible lipid that the cells accumulate. There is a need for the development of a more uniform staining procedure. In its first phase, this study examined the dependence of microalgal Nile red fluorescence (Tetraselmis suecica) in terms of its most pertinent staining variables. A quadratic surface model that successfully described the Nile red fluorescence intensity as a composite function of its variables was generated (r2=0.86). Cell concentration was shown to have a significant effect on the fluorescence intensity. Up to a certain threshold, fluorescence intensity was shown to increase with Nile red dye concentration. In its second phase, the study reviewed findings from previous Nile red studies to elucidate some of the fundamental mechanism underlying the diffusion of Nile red dye molecules into the microalgal cells and their subsequent interaction with intracellular lipids. Through the review process, we were able to develop a simple framework that provided a set of guidelines for the standardization of the Nile red staining procedure across different microalgal species.

Nile Red – contrasting colour cyanoacrylate

2015 ◽  
Vol 290 ◽  
pp. 115-120
Author(s):  
Ewa Rogoża ◽  
◽  
Katarzyna Drzewiecka ◽  
Keyword(s):  
Spectral Analysis ◽  
Fluorescent Dyes ◽  
Nile Red ◽  
Laboratory Practice ◽  
Excitation Light ◽  
Red Fluorescence ◽  
Yellow Orange ◽  
Nile Red Fluorescence ◽  
Filter Selection

Fingerprints disclosed by cyanoacrylate on non-absorbent substrates, in order to improve readabiIity, require additional contrasting fluorescent dyes. Nile Red is one of them. Its effectiveness was tested under conditions similar to those of daily laboratory practice. The highest Nile Red fluorescence occurred in an excitation light of blue-green and a wavelength of 505nm. Spectral analysis showed that the emission of light oscillates in the wavelength of about 630nm. In order to cut off the light, longpass edge filters of the colours yellow, orange or red can be used. Filter selection depends on the characteristics of the substrate and can be chosen empirically. Nile Red fluorescence does not change over a longer period of time, which allows for the registration of fingerprints to be performed within a time convenient for the testing, without fear of losing their quality. Nile Red may be an alternative to other fluorescent dyes used for visualization in fingerprint testing.

scholarly journals An improved high-throughput Nile red fluorescence assay for estimating intracellular lipids in a variety of yeast species

2012 ◽  
Vol 91 (2) ◽  
pp. 321-328 ◽  
Author(s):  
I.R. Sitepu ◽  
L. Ignatia ◽  
A.K. Franz ◽  
D.M. Wong ◽  
S.A. Faulina ◽  
...  
Keyword(s):  
High Throughput ◽  
Yeast Species ◽  
Nile Red ◽  
Fluorescence Assay ◽  
Red Fluorescence ◽  
Intracellular Lipids ◽  
Nile Red Fluorescence
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