Inexpensive Adaptations of Basic Microscopes for the Identification of Microplastic Contamination Using Polarization and Nile Red Fluorescence Detection

2020 ◽  
Vol 97 (11) ◽  
pp. 4026-4032
Author(s):  
Amelia B. Labbe ◽  
Clive R. Bagshaw ◽  
Lisa Uttal
Keyword(s):  
Fluorescence Detection ◽  
Nile Red ◽  
Red Fluorescence ◽  
Nile Red Fluorescence

Characteristic features of nile red fluorescence in transparent xerogels

2015 ◽  
Vol 49 (4) ◽  
pp. 249-254 ◽  
Author(s):  
M. S. Pilipenko ◽  
A. V. Koshkin ◽  
V. A. Sazhnikov ◽  
M. V. Alfimov
Keyword(s):  
Nile Red ◽  
Red Fluorescence ◽  
Nile Red Fluorescence ◽  
Characteristic Features

scholarly journals Nile Red Staining for Oil Determination in Microalgal Cells: A New Insight through Statistical Modelling

2015 ◽  
Vol 2015 ◽  
pp. 1-14 ◽  
Author(s):  
Ronald Halim ◽  
Paul A. Webley
Keyword(s):  
Fluorescence Intensity ◽  
Nile Red ◽  
Biofuel Production ◽  
Staining Procedure ◽  
Surface Model ◽  
Second Phase ◽  
Red Fluorescence ◽  
Nile Red Fluorescence ◽  
Red Dye ◽  
Nile Red Staining

In the wake of global warming and rapid fossil fuel depletion, microalgae emerge as promising feedstocks for sustainable biofuel production. Nile red staining acts as a rapid diagnostic tool to measure the amount of biodiesel-convertible lipid that the cells accumulate. There is a need for the development of a more uniform staining procedure. In its first phase, this study examined the dependence of microalgal Nile red fluorescence (Tetraselmis suecica) in terms of its most pertinent staining variables. A quadratic surface model that successfully described the Nile red fluorescence intensity as a composite function of its variables was generated (r2=0.86). Cell concentration was shown to have a significant effect on the fluorescence intensity. Up to a certain threshold, fluorescence intensity was shown to increase with Nile red dye concentration. In its second phase, the study reviewed findings from previous Nile red studies to elucidate some of the fundamental mechanism underlying the diffusion of Nile red dye molecules into the microalgal cells and their subsequent interaction with intracellular lipids. Through the review process, we were able to develop a simple framework that provided a set of guidelines for the standardization of the Nile red staining procedure across different microalgal species.

Nile Red – contrasting colour cyanoacrylate

2015 ◽  
Vol 290 ◽  
pp. 115-120
Author(s):  
Ewa Rogoża ◽  
◽  
Katarzyna Drzewiecka ◽  
Keyword(s):  
Spectral Analysis ◽  
Fluorescent Dyes ◽  
Nile Red ◽  
Laboratory Practice ◽  
Excitation Light ◽  
Red Fluorescence ◽  
Yellow Orange ◽  
Nile Red Fluorescence ◽  
Filter Selection

Fingerprints disclosed by cyanoacrylate on non-absorbent substrates, in order to improve readabiIity, require additional contrasting fluorescent dyes. Nile Red is one of them. Its effectiveness was tested under conditions similar to those of daily laboratory practice. The highest Nile Red fluorescence occurred in an excitation light of blue-green and a wavelength of 505nm. Spectral analysis showed that the emission of light oscillates in the wavelength of about 630nm. In order to cut off the light, longpass edge filters of the colours yellow, orange or red can be used. Filter selection depends on the characteristics of the substrate and can be chosen empirically. Nile Red fluorescence does not change over a longer period of time, which allows for the registration of fingerprints to be performed within a time convenient for the testing, without fear of losing their quality. Nile Red may be an alternative to other fluorescent dyes used for visualization in fingerprint testing.

scholarly journals Modified high-throughput Nile red fluorescence assay for the rapid screening of oleaginous yeasts using acetic acid as carbon source

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Catarina Miranda ◽  
Sara Bettencourt ◽  
Tatiana Pozdniakova ◽  
Joana Pereira ◽  
Paula Sampaio ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Carbon Source ◽  
High Throughput ◽  
Nile Red ◽  
Fluorescence Assay ◽  
Rapid Screening ◽  
Oleaginous Yeasts ◽  
Red Fluorescence ◽  
Nile Red Fluorescence
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