Cytotoxic alkaloids from the marine shellfish-associated fungus Aspergillus sp. XBB-4 induced by an amino acid-directed strategy

Aspergillus sp. strain O20 produces inducible D-amino acid oxidase intracellularly, only in the presence of some amino acids. The enzyme was induced most effectively by the addition of DL-alanine (1% w/v) to the production medium. Among the various compounds studied, production of the D-amino acid oxidase was enhanced by Aerosol-22, glucose, and sodium nitrate. D-Amino acid oxidase formation was observed during the onset of the stationary growth phase. Maximum enzyme activity was recorded after 96 h of fermentation (1000 IU/L).Key words: D-amino acid oxidase, Aspergillus sp., 7-aminocephalosporanic acid, cephalosporin C.

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Isolation, 1H, 13C NMR Assignments, and crystal structure of chrodrimanin B from A marine fungus Aspergillus sp.

Chemistry of Natural Compounds ◽

10.1007/s10600-011-9997-y ◽

2011 ◽

Vol 47 (4) ◽

pp. 571-573 ◽

Cited By ~ 16

Author(s):

Mei-Yan Wei ◽

Guang-Ying Chen ◽

Yu Wang ◽

Xiu-Li Zhang ◽

Chang-Yun Wang ◽

...

Keyword(s):

Crystal Structure ◽

13C Nmr ◽

Nmr Assignments ◽

Marine Fungus ◽

Aspergillus Sp

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Anti-neuroinflammatory effect of aurantiamide acetate from the marine fungus Aspergillus sp. SF-5921: Inhibition of NF-κB and MAPK pathways in lipopolysaccharide-induced mouse BV2 microglial cells

International Immunopharmacology ◽

10.1016/j.intimp.2014.10.006 ◽

2014 ◽

Vol 23 (2) ◽

pp. 568-574 ◽

Cited By ~ 28

Author(s):

Chi-Su Yoon ◽

Dong-Cheol Kim ◽

Dong-Sung Lee ◽

Kyoung-Su Kim ◽

Wonmin Ko ◽

...

Keyword(s):

Marine Fungus ◽

Microglial Cells ◽

Mapk Pathways ◽

Aspergillus Sp ◽

Aurantiamide Acetate ◽

Bv2 Microglial Cells

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New asymmetrical bispyrrolidinoindoline diketopiperazines from the marine fungus Aspergillus sp. DX4H

Natural Product Research ◽

10.1080/14786419.2017.1363752 ◽

2017 ◽

Vol 32 (7) ◽

pp. 815-820 ◽

Cited By ~ 9

Author(s):

Jinzhong Xu ◽

Qian Hu ◽

Wanjing Ding ◽

Pinmei Wang ◽

Yanan Di

Keyword(s):

Marine Fungus ◽

Aspergillus Sp

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Asperginine, an Unprecedented Alkaloid from the Marine-derived Fungus Aspergillus sp

Natural Product Communications ◽

10.1177/1934578x1501000812 ◽

2015 ◽

Vol 10 (8) ◽

pp. 1934578X1501000 ◽

Cited By ~ 1

Author(s):

Pinmei Wang ◽

Shizhe Zhao ◽

Ying Liu ◽

Wanjing Ding ◽

Feng Qiu ◽

...

Keyword(s):

Prostate Cancer ◽

Spectral Data ◽

Absolute Configuration ◽

Marine Fungus ◽

Mass Spectral Data ◽

Hydrogen Atoms ◽

Relative Configuration ◽

Mass Spectral ◽

Mtt Method ◽

Aspergillus Sp

Asperginine (1), an alkaloid possessing a rare skeleton, was isolated from the cultural broth of the marine fungus Aspergillus sp. (Z-4) isolated from the gut of the marine isopod Ligia oceanica. The planar structure and relative configuration of 1 was determined by analysis of NMR and mass spectral data. Its absolute configuration was elucidated by Marfey's method, together with NOESY correlations of key hydrogen atoms. The cytotoxicity against prostate cancer PC3 and human HCT116 was assayed by the MTT method. Unfortunately, asperginine did not show any activity.

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Protective Effects of Emodin and Chrysophanol Isolated from Marine FungusAspergillus sp.on Ethanol-Induced Toxicity in HepG2/CYP2E1 Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2011/452621 ◽

2011 ◽

Vol 2011 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Zhong-Ji Qian ◽

Chen Zhang ◽

Yong-Xin Li ◽

Jae-Young Je ◽

Se-Kwon Kim ◽

...

Keyword(s):

Culture Media ◽

Fatty Infiltration ◽

Industrial Applications ◽

Marine Fungus ◽

Potential Candidate ◽

Protective Effects ◽

Gamma Glutamyl Transpeptidase ◽

Irreversible Damage ◽

Aspergillus Sp

Alcohol-induced liver injury progresses from fatty infiltration followed by a harmful cause of inflammation leading to an irreversible damage. In this study, two compounds (emodin and chrysophanol) isolated from marine fungusAspergillus sp.were examined for their protective effects against ethanol-induced toxicityin vitro. Ethanol-induced HepG2/CYP2E1 cells were treated with the compounds at various concentrations, and the results showed that there was a dose-dependent decrease of gamma-glutamyl transpeptidase (GGT) activity and increase of glutathione (GSH) in the culture media with an increase in cell viability. Furthermore, the protective effects of the compounds were evaluated by protein expression levels of GGT, GSH, and CYP2E1 using Western blot. Among the compounds, emodin addressed to the ethanol-induced cytotoxicity more effectively compared to the chrysophanol. It could be suggested that emodin isolated from this genus would be a potential candidate for attenuating ethanol induced liver damage for further industrial applications such as functional food and pharmaceutical developments.

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Rapid separation and purification of two C25 steroids with bicyclic [4.4.1] A/B rings from the marine fungus Aspergillus sp. LS116 by high-speed counter-current chromatography in stepwise elution mode

Natural Product Research ◽

10.1080/14786419.2021.1885404 ◽

2021 ◽

pp. 1-5

Author(s):

Zhende Dou ◽

Xiaoping He ◽

Peng Xu ◽

Bin Zhang ◽

Lijian Ding

Keyword(s):

High Speed ◽

Marine Fungus ◽

Separation And Purification ◽

Rapid Separation ◽

Aspergillus Sp ◽

Counter Current

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Alkaloids From the Marine Fungus Lecanicillium fusisporum Using an Amino Acid-Directed Strategy

Natural Product Communications ◽

10.1177/1934578x211046072 ◽

2021 ◽

Vol 16 (10) ◽

pp. 1934578X2110460

Author(s):

Qin Huang ◽

Hou-Jin Li ◽

Chang-Bai Huang ◽

Zi-Han Wang ◽

Wen-Jian Lan ◽

...

Keyword(s):

Amino Acid ◽

Spectroscopic Data ◽

Marine Fungi ◽

Optical Rotation ◽

Indole Alkaloid ◽

2D Nmr ◽

Marine Fungus ◽

Culture Broth ◽

Synthetic Approach ◽

C Nmr

An amino acid-directed strategy has been developed to explore the potential of marine fungi to produce alkaloids. The marine fungus Lecanicillium fusisporum was cultured in glucose-peptone-yeast (GPY) medium to which were added L-tryptophan, L-phenylalanine, L-threonine, D, L-methionine, L-serine, L-lysine and L-valine. A new indole alkaloid, lecasporinoid (1), along with five known alkaloids (2−6) were discovered from the culture broth. The planar structure of lecasporinoid (1) was determined by HR-ESIMS, and 1D and 2D NMR spectroscopic data. The absolute configuration was established by optical rotation and 13C NMR calculations combining with a chemical synthetic approach.

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