scholarly journals High salt compatible oxyanion receptors by dual ion imprinting

2020 ◽  
Vol 11 (16) ◽  
pp. 4246-4250 ◽  
Author(s):  
Sudhirkumar Shinde ◽  
Mona Mansour ◽  
Anil Incel ◽  
Liliia Mavliutova ◽  
Celina Wierzbicka ◽  
...  
Keyword(s):  
Ionic Strength ◽  
Ion Pair ◽  
High Ionic Strength ◽  
High Salt ◽  
Ion Uptake ◽  
Ion Imprinting

Imprinting of an ion-pair in presence of mutually compatible anion and cation host monomers leads to polymers showing enhanced ion uptake in competitive high ionic strength buffers.

Structure of bacteriorhodopsin in the acidified membrane and at high ionic strength: resonance Raman study

1985 ◽  
Vol 63 (7) ◽  
pp. 2012-2017 ◽  
Author(s):  
G. Massig ◽  
M. Stockburger ◽  
Th. Alshuth
Keyword(s):  
Ionic Strength ◽  
Resonance Raman ◽  
Ion Pair ◽  
High Ionic Strength ◽  
Original Structure ◽  
Water Molecules ◽  
Native State ◽  
Surrounding Water ◽  
Acidic Form ◽  
Raman Study

Resonance Raman (RR) spectra of the retinylidene Schiffs base (SB) chromophore of bacteriorhodopsin (BR) were obtained from purple membrane (PM) suspensions of Halobacteriumhalobium in three different equilibrium states: (1) the native state at neutral pH; (2) BR-605, the acidified membrane at pH 2; (3) BR-565(Cl−) or BR-545 (F−), at low pH and high ionic strength. In the native state the retinyl chromophore is stabilized by an ion-pair complex of the protonated SB group with a negative carboxylate counterion (A−) of the protein, giving [Formula: see text]. In the acidic form, BR-605, it is inferred from the RR spectra that the chromophore is immobilized. This is ascribed to a breakdown of the ionic interaction by protonation of A−. In the two (3) forms the RR spectra show that the original structure of the chromophore in the native state is reestablished, which is attributed to the formation of [Formula: see text] complexes with the anions. It thus turns out from RR spectroscopic evidence that the retinyl chromophore in BR is largely governed by the [Formula: see text] ion-pair formation, which is stabilized by surrounding water molecules.

Importance of Protease Inhibition in Studies on Purified Factor VIII (Antihaemophilic Factor)

1976 ◽  
Vol 35 (01) ◽  
pp. 186-190 ◽  
Author(s):  
Eugen A. Beck ◽  
Peter Bachmann ◽  
Peter Barbier ◽  
Miha Furlan
Keyword(s):  
Ionic Strength ◽  
Factor Viii ◽  
Gel Filtration ◽  
High Ionic Strength ◽  
Procoagulant Activity ◽  
Carrier Protein ◽  
Protease Inhibition ◽  
Functional Sites ◽  
Molecular Weight Peak ◽  
Von Willebrand

SummaryAccording to some authors factor VIII procoagulant activity may be dissociable from carrier protein (MW~ 2 × 106) by agarose gel filtration, e.g. at high ionic strength. We were able to reproduce this phenomenon. However, addition of protease inhibitor (Trasylol) prevented the appearance of low molecular weight peak of factor VIII procoagulant activity both at high ionic strength and elevated temperature (37°C). We conclude from our results that procoagulant activity and carrier protein (von Willebrand factor, factor VIII antigen) are closely associated functional sites of native factor VIII macro molecule. Consequently, proteolytic degradation should be avoided in functional and structural studies on factor VIII and especially in preparing factor VIII concentrate for therapeutic use.

THE RELATIVE DISTRIBUTION OF THYROXINE, TRIIODOTHYRONINE AND 3,3′,5′-(REVERSE)-TRIIODOTHYRONINE IN VARIOUS FRACTIONS OF THYROGLOBULIN

1978 ◽  
Vol 88 (2) ◽  
pp. 298-305 ◽  
Author(s):  
Peter Laurberg
Keyword(s):  
Ionic Strength ◽  
Guinea Pig ◽  
Sucrose Gradient ◽  
Deae Cellulose ◽  
High Ionic Strength ◽  
Relative Distribution ◽  
Thyroid Extract ◽  
Reverse Triiodothyronine ◽  
Thyroid Secretion ◽  
Stable Iodine

ABSTRACT Thyroglobulin fractions rich and poor in new thyroglobulin were separated by means of DEAE-cellulose chromatography of dog thyroid extracts and by zonal ultracentrifugation in a sucrose gradient of guinea pig thyroid extract incubated at low temperature. The distribution of thyroxine, triiodothyronine and 3,3′,5′-(reverse)-triiodothyronine in hydrolysates of the different fractions was estimated by radioimmunoassays. Following DEAE-cellulose chromatography there was a small but statistically significant increase in the T4/T3 ratio in thyroglobulin fractions eluted at high ionic strength - that is fractions relatively rich in stable iodine but poor in fresh thyroglobulin. There were no differences in the T4/rT3 ratios between the different fractions. The ratios between iodothyronines were almost identical in the various thyroglobulin fractions following zonal ultracentrifugation in a sucrose gradient of cold treated guinea pig thyroid extract. These findings lend no support to the possibility that a relatively high content of triiodothyronines in freshly synthesized thyroglobulin modulates the thyroid secretion towards a preferential secretion of triiodothyronine and 3,3′,5′-(reverse)-triiodothyronine at the expense of the secretion of thyroxine.

scholarly journals Kaolin based protective barrier in municipal landfills against adverse chemo-mechanical loadings

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Partha Das ◽  
Tadikonda Venkata Bharat
Keyword(s):  
Ionic Strength ◽  
High Ionic Strength ◽  
The Self ◽  
Design Parameters ◽  
Layered System ◽  
Geosynthetic Clay Liners ◽  
Clay Liners ◽  
Landfill Liner ◽  
First Time ◽  
Applied Stresses

AbstractIn this work, we assess the self-sealing and swelling ability of the compacted granular bentonite (GB) under an inorganic salt environment and induced overburden stresses from the landfill waste. The laboratory permeation tests with high ionic strength salt solutions reveal that the GB fails to seal and exhibits a significant mechanical collapse under different applied stresses. The applicability of GB in the form of geosynthetic clay liners as the bottom liner facilities in landfills that produce high ionic strength salt leachates, therefore, remains a serious concern. We propose an additional barrier system based on kaolin, for the first time, to address this problem. The proposed kaolin-GB layered system performs satisfactorily in terms of its sealing and swelling ability even in adverse saline conditions and low overburden stresses. The kaolin improves the osmotic efficiency of the self and also helps the underlying GB layer to seal the inter-granular voids. The estimated design parameters by through-diffusion test suggest that the kaolin-GB layered system effectively attenuates the permeant flux and suitable as a landfill liner.

scholarly journals High ionic strength dissociation assay (HISDA) for high drug tolerant immunogenicity testing

Bioanalysis ◽  
2020 ◽  
Author(s):  
Gregor Jordan ◽  
Alexander Pöhler ◽  
Florence Guilhot ◽  
Meike Zaspel ◽  
Roland F Staack
Keyword(s):  
Ionic Strength ◽  
Acid Treatment ◽  
Structural Integrity ◽  
Drug Tolerance ◽  
High Ionic Strength ◽  
Acid Dissociation ◽  
High Drug ◽  
Overnight Incubation ◽  
Antidrug Antibody ◽  
High Doses

Aim: Antidrug antibody (ADA) assessment may be challenged in studies that involve the administration of high doses of biotherapeutics and/or with long half-lives. In such cases, ADA assays with optimized drug tolerance are desired. Material & Methods: We evaluated the use of MgCl2 to develop high ionic strength dissociation assays in two investigational examples (bridging enzyme-linked immunosorbent ADA assays) to attain high drug tolerance while maintaining best possible structural integrity of ADAs. Results: Both ADA-bridging assays treated with MgCl2 showed improved drug tolerance and higher signal-to-blank values compared with overnight incubation or acid treatment. Conclusion: The use of MgCl2 treatment in ADA-bridging assays provides a sensitive, drug tolerant and easy-to-use alternative in cases where acid dissociation is not possible or unwanted.

scholarly journals Denaturation of Alaska pollack myofibrils induced by CaCl2 at high ionic strength.

1989 ◽  
Vol 55 (1) ◽  
pp. 173-178
Author(s):  
Hiroki Saeki ◽  
Hirotada Ozaki ◽  
Michio Nonaka ◽  
Ken-ichi Arai
Keyword(s):  
Ionic Strength ◽  
High Ionic Strength

Precipitation of calcium oxalates from high ionic strength solutions

1987 ◽  
Vol 80 (1) ◽  
pp. 113-120 ◽  
Author(s):  
Drago Škrtić ◽  
Helga Füredi-Milhofer ◽  
Milenko Marković
Keyword(s):  
Ionic Strength ◽  
High Ionic Strength ◽  
Calcium Oxalates
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