A critical view at the ISO 13528 and IUPAC's harmonized protocol approach for proficiency testing for homogeneity assessment for quantitative variables

The Analyst ◽  
2020 ◽  
Vol 145 (23) ◽  
pp. 7630-7635
Author(s):  
Wim Coucke ◽  
Mohamed Rida Soumali
Keyword(s):  
High Risk ◽  
Proficiency Testing ◽  
Homogeneity Test ◽  
Critical View ◽  
Homogeneity Assessment ◽  
Iso 13528 ◽  
Sample Homogeneity

Current PT sample homogeneity test has high risk of accepting heterogeneous.

Introduction to Statistical Methods for Outlier Detection and Sample Homogeneity Assessment of Reference Materials and Proficiency Test Items

2021 ◽  
Author(s):  
Yi-Ting Chen ◽  
Keyword(s):  
Data Analysis ◽  
Outlier Detection ◽  
Reference Materials ◽  
Proficiency Test ◽  
Test Items ◽  
Homogeneity Assessment ◽  
Iso 13528 ◽  
Scope Of Application ◽  
Iso 5725 ◽  
Sample Homogeneity

Due to the homogeneity of the product or sample, it will affect whether it meets the scope of application and purpose. For example, the reference materials(RM) produced by the reference material producer(RMP), and the proficiency test items selected by the proficiency testing provider(PTP), in order to ensure the reference materials or proficiency test items have consistent characteristics or comparability, they should be proved to have certain homogeneity. However, before performing homogeneity assessment, it is necessary to measure the characteristic parameters of the reference materials or proficiency test items to obtain a sufficient number of measured values for data analysis, but there may be outliers in the measured values that may affect data analysis and interpretation of the results. Therefore, this article will refer to ASTM E178-16a:2016[1], ISO 5725-2:1994[2], ISO 13528:2015[3], etc., to introduce several outlier detection and homogeneity assessment methods, supplemented by case studies. Finally, this article will remind the precautions for the use of the method, so that readers can choose the appropriate method for use in the actual analysis.

Alternative Sample-Homogeneity Test for Quantitative and Qualitative Proficiency Testing Schemes

2019 ◽  
Vol 91 (3) ◽  
pp. 1847-1854
Author(s):  
Wim Coucke ◽  
Jelena Vlašić Tanasković ◽  
Lobna Bouacida ◽  
Sylvia Broeders ◽  
Bernard China ◽  
...  
Keyword(s):  
Proficiency Testing ◽  
Homogeneity Test ◽  
Proficiency Testing Schemes ◽  
Sample Homogeneity

scholarly journals Proficiency Testing Program for the Determination of Total Arsenic, Cadmium, and Lead in Seawater Shrimp

2008 ◽  
Vol 91 (4) ◽  
pp. 858-864 ◽  
Author(s):  
Mei-Fung Kong ◽  
Serena Chan ◽  
Yiu-Chung Wong
Keyword(s):  
Proficiency Testing ◽  
Robust Statistics ◽  
Asia Pacific ◽  
Homogeneity Test ◽  
Total Arsenic ◽  
Mean Values ◽  
True Value ◽  
Consensus Mean ◽  
Cadmium And Lead

Abstract The proficiency testing (PT) program for 97 worldwide laboratories for determining total arsenic, cadmium, and lead in seawater shrimp under the auspices of the Asia-Pacific Laboratory Accreditation Cooperation (APLAC) is discussed. The program is one of the APLAC PT series whose primary purposes are to establish mutual agreement on the equivalence of the operation of APLAC member laboratories and to take corrective actions if testing deficiencies are identified. Pooled data for Cd and Pb were normally distributed with interlaboratory variations of 21.9 and 34.8, respectively. The corresponding consensus mean values estimated by robust statistics were in good agreement with those obtained in the homogeneity tests. However, a bimodal distribution was observed from the determination of total As, in which 14 out of 74 participants reported much smaller values (0.4826.4 mg/kg) as compared with the mean values of 60.9 mg/kg in the homogeneity test. The use of consensus mean is known to have significant deviation from the true value in bi- or multimodal distribution. Therefore, the mode value, a better estimate of central tendency, was chosen to assess participants' performance for total As. Estimates of the overall uncertainty from participants varied in this program, and some were recommended to acquire more comprehensive exposure toward important criteria as stipulated in ISO/IEC 17025.

scholarly journals Proficiency testing for the calibration of masses

2019 ◽  
Author(s):  
Lise HEGRON ◽  
Boris GEYNET
Keyword(s):  
Proficiency Testing ◽  
European Countries ◽  
Testing Laboratories ◽  
Iso 13528 ◽  
Mass Calibration ◽  
Calibration Laboratories

The CT2M organized in 2018 a european inter-laboratory comparison (ILC) for the calibration of masses. The proficiency testing was particulary intended for the calibration laboratories (accredited or not) but also the testing laboratories carrying out their own calibrations and / or controls of their masses. This circuit took place between April 2018 to October 2018 in five European countries: England, France, Germany, Portugal and Switzerland. The results were processed according to the statistical principle of ISO 13528 [1] and in compliance with the requirements of ISO 17043 [2]. This article presents the organization of this inter-laboratory comparison and the results. The performances of the participants are evaluated and an interpretation of the results is proposed in order to highlight the predominant influence parameters on the mass calibration results (nominal values: 200mg, 2g, 20g, 200g and 20kg).

scholarly journals Comparative Performance of High-Risk Human Papillomavirus RNA and DNA In Situ Hybridization on College of American Pathologists Proficiency Tests

2019 ◽  
Vol 144 (3) ◽  
pp. 344-349 ◽  
Author(s):  
Elaine S. Keung ◽  
Rhona J. Souers ◽  
Julia A. Bridge ◽  
William C. Faquin ◽  
Rondell P. Graham ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
High Risk ◽  
Proficiency Testing ◽  
Analytical Sensitivity ◽  
Proficiency Tests ◽  
Comparative Performance ◽  
Specific Test ◽  
Rna And Dna

Context.— Detection of high-risk human papillomavirus (HR-HPV) in squamous cell carcinoma is important for classification and prognostication. In situ hybridization (ISH) is a commonly used HR-HPV–specific test that targets viral RNA or DNA. The College of American Pathologists (CAP) provides proficiency testing for laboratories performing HR-HPV ISH. Objective.— To compare the analytical performance of RNA- and DNA-based ISH methods on CAP HR-HPV proficiency tests. Design.— Data from the 2016–2018 CAP HPV ISH proficiency testing surveys were reviewed. These surveys consist of well-characterized samples with known status for HR-HPV, including 1 to 2 copies, 50 to 100 copies, 300 to 500 copies, and no copies of HR-HPV per cell. Results.— Ninety-five participants submitted 1268 survey results from 20 cores. Overall, RNA ISH had a significantly higher percentage of correct responses than DNA ISH: 97.4% (450 of 462) versus 80.6% (650 of 806) (P < .001). This disparity appears to be the consequence of a superior sensitivity of RNA ISH compared to DNA ISH for samples with 1 to 2 and with 50 to 100 copies of HR-HPV per cell: 95.2% (120 of 126) versus 53.8% (129 of 240), P < .001, respectively, and 100% (89 of 89) versus 76.3% (119 of 156), P < .001, respectively. Conclusions.— An assessment of CAP HR-HPV proficiency test performance indicates that RNA ISH shows significantly higher accuracy than DNA ISH owing to higher analytical sensitivity of RNA ISH in tumors with low (1–2 copies per cell) to intermediate (50–100 copies per cell) HR-HPV viral copy numbers. These data support the use of RNA over DNA ISH in clinical laboratories that perform HR-HPV testing as part of their testing algorithms.

scholarly journals The College of American Pathologists' First 3 Years' Experience With High-Risk Human Papillomavirus Proficiency Testing for Cytology and Other Laboratories

2013 ◽  
Vol 137 (5) ◽  
pp. 606-609 ◽  
Author(s):  
Ann T. Moriarty ◽  
Joel S. Bentz ◽  
Barbara Winkler ◽  
Andrew H. Fischer ◽  
Rodolfo Laucirica ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Proficiency Testing

scholarly journals Homogeneity and Stability of Samples Used for Proficiency Testing in Enumeration of Coagulase Positive Staphylococci

2012 ◽  
Vol 56 (4) ◽  
pp. 559-562 ◽  
Author(s):  
Weronika Korpysa-Dzirba ◽  
Jolanta G. Rola ◽  
Jacek Osek
Keyword(s):  
Proficiency Testing ◽  
Horse Serum ◽  
Skim Milk ◽  
Proficiency Tests ◽  
Powdered Milk ◽  
Preliminary Results ◽  
Freeze Dried ◽  
Iso 13528 ◽  
Coagulase Positive Staphylococci

Abstract This study describes preparation of test samples composed of freeze-dried strain of S. aureus and powdered milk as a matrix. In the first part of the study, the number of S. aureus cells freeze-dried in skim milk or horse serum were compared at two levels of contamination (104 and 105 cfu g-1). The analysis of the samples was performed three times within a week. The preliminary results showed that the samples composed of S. aureus freeze-dried in horse serum were more stable and homogeneous than those prepared with skim milk. These results were further confirmed after analysing a higher number of such samples. Therefore, this procedure was then chosen for preparation of the samples for proficiency tests (PTs). Homogeneity and stability of these samples were checked according to ISO 13528. The results obtained showed that the samples met the criteria of stability and homogeneity required for PTs and were used in PT for enumeration of S. aureus in powdered milk.

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