Luminous silica colloids with carbon dot incorporation for sensitive immunochromatographic assay of Zika virus

The Analyst ◽  
2021 ◽  
Author(s):  
Lai-Di Xu ◽  
Feng-Luan Du ◽  
Jin Zhu ◽  
Shou-Nian Ding
Keyword(s):  
Zika Virus ◽  
Virus Detection ◽  
Immunochromatographic Assay ◽  
Silica Spheres ◽  
Carbon Dot ◽  
Silica Colloids

Fluorescent SiO2 colloids prepared from silanized CDs embedded in silica spheres were integrated with an immunochromatographic platform for Zika virus detection.

Enhanced electrochemiluminescence of CdS quantum dots capped with mercaptopropionic acid activated by EDC for Zika virus detection

The Analyst ◽  
2021 ◽  
Author(s):  
Hui-Jun Zhang ◽  
Jin Zhu ◽  
Ning Bao ◽  
Shou-Nian Ding
Keyword(s):  
Quantum Dots ◽  
Zika Virus ◽  
Virus Detection ◽  
Cds Quantum Dots ◽  
Mercaptopropionic Acid ◽  
Sandwich Type ◽  
Cds Qds

The mechanism of enhanced ECL of MPA@CdS QDs by EDC activation was investigated, and a sandwich-type ECL immunosensor has been designed for Zika virus detection.

scholarly journals Development of a Colloidal Gold Immunochromatographic Assay for Duck Enteritis Virus Detection Using Monoclonal Antibodies

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 365
Author(s):  
Fengli Liu ◽  
Yanxin Cao ◽  
Maokai Yan ◽  
Mengxu Sun ◽  
Qingshui Zhang ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Colloidal Gold ◽  
Preventive Intervention ◽  
Virus Detection ◽  
Cross Reactivity ◽  
Duck Enteritis Virus ◽  
Detection Sensitivity ◽  
Immunochromatographic Assay ◽  
Efficient Detection ◽  
Enteritis Virus

Duck viral enteritis is a highly contagious and fatal disease of commercial waterfowl flocks. The disease occurs sporadically or epizootically in mainland China due to insufficient vaccinations. Early and rapid diagnosis is important for preventive intervention and the control of epizootic events in clinical settings. In this study, we generated two monoclonal antibodies (MAbs) that specifically recognized the duck enteritis virus (DEV) envelope glycoprotein B and tegument protein UL47, respectively. Using these MAbs, a colloidal gold-based immunochromatographic assay (ICA) was developed for the efficient detection of DEV antigens within 15 min. Our results showed that the detection limit of the developed ICA strip was 2.52 × 103 TCID50/mL for the virus infected cell culture suspension with no cross-reactivity with other pathogenic viruses commonly encountered in commercially raised waterfowl. Using samples from experimentally infected ducks, we demonstrated that the ICA detected the virus in cloacal swab samples on day three post-infection, demonstrating an 80% concordance with the PCR. For tissue homogenates from ducks succumbing to infection, the detection sensitivity was 100%. The efficient and specific detection by this ICA test provides a valuable, convenient, easy to use and rapid diagnostic tool for DVE under both laboratory and field conditions.

A phenylenediamine-based carbon dot-modified silica stationary phase for hydrophilic interaction chromatography

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 1056-1061 ◽  
Author(s):  
Yali Yang ◽  
Haijuan Zhang ◽  
Jia Chen ◽  
Zhan Li ◽  
Liang Zhao ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Carbon Dots ◽  
Porous Silica ◽  
Hydrophilic Interaction Chromatography ◽  
Silica Spheres ◽  
Hydrophilic Interaction ◽  
Modified Silica ◽  
Carbon Dot ◽  
New Stationary Phase ◽  
Enhanced Selectivity

Red emitting carbon dots derived from p-phenylenediamine were successfully grafted onto the surface of porous silica spheres which served as a new stationary phase for hydrophilic interaction chromatography with enhanced selectivity.

scholarly journals Zika virus detection using antibody-immobilized disposable cover glass and AlGaN/GaN high electron mobility transistors

2018 ◽  
Vol 113 (3) ◽  
pp. 032101 ◽  
Author(s):  
Jiancheng Yang ◽  
Patrick Carey ◽  
Fan Ren ◽  
Michael A. Mastro ◽  
Kimberly Beers ◽  
...  
Keyword(s):  
Electron Mobility ◽  
Zika Virus ◽  
Virus Detection ◽  
High Electron Mobility Transistors ◽  
High Electron ◽  
Cover Glass ◽  
High Electron Mobility ◽  
Electron Mobility Transistors

Preparation of His-Tagged Armored RNA particles as positive controls for Real-Time RT-PCR Detection of Zika virus

2021 ◽  
Vol 31 (4) ◽  
pp. 18-27
Author(s):  
Do Thi Quynh Nga ◽  
Ha Thi Phuong Mai ◽  
Tran Thi Hai Au ◽  
Vu Thi Kim Lien ◽  
Vu Thi Bich Hau ◽  
...  
Keyword(s):  
Real Time ◽  
Zika Virus ◽  
Room Temperature ◽  
Virus Detection ◽  
Positive Control ◽  
Reverse Transcription Pcr ◽  
His Tag ◽  
Armored Rna ◽  
Genomic Regions ◽  
Positive Controls

Armored RNA (AR) is a good candidate for creating nuclease-resistant RNA positive controls in the nucleic acid - based assay for RNA viruses. To simplify the production and purifcation of armored RNA, a single plasmid double – expressing His6-tag system was designed. His-tag armored RNA particles were purifed using his-tag affnity. A genomic fragment of the zika virus consisting of the encoding sequences of flavi-M, flavi-E-C protein targeted for zika virus was selected to prepare a positive control. In this study, we have successfully produced His-taged MS2- phage like particles carrying specifc genomic regions (M and E genes) to monitor the procedures of real-time Reverse transcription-PCR for Zika virus detection in one plasmid double expression. AR-ZIKA is completely resistant to DNase and RNase, stable in normal human EDTA plasma at room temperature for at least 60 and 15 days at 40C and room temperature respectively.

scholarly journals Human saliva can be a diagnostic tool for Zika virus detection

2019 ◽  
Vol 12 (5) ◽  
pp. 601-604 ◽  
Author(s):  
Zohaib Khurshid ◽  
Muhammad Zafar ◽  
Erum Khan ◽  
Maria Mali ◽  
Muhammad Latif
Keyword(s):  
Diagnostic Tool ◽  
Zika Virus ◽  
Virus Detection ◽  
Human Saliva

A sensitive label-free impedimetric DNA biosensor based on silsesquioxane-functionalized gold nanoparticles for Zika Virus detection

2019 ◽  
Vol 141 ◽  
pp. 111351 ◽  
Author(s):  
Marines Steinmetz ◽  
Dhésmon Lima ◽  
Adriano Gonçalves Viana ◽  
Sérgio Toshio Fujiwara ◽  
Christiana Andrade Pessôa ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Zika Virus ◽  
Virus Detection ◽  
Dna Biosensor ◽  
Label Free ◽  
Functionalized Gold Nanoparticles

scholarly journals Persistent Zika Virus Detection in Semen in a Traveler Returning to the United Kingdom from Brazil, 2016

2017 ◽  
Vol 23 (1) ◽  
pp. 137-139 ◽  
Author(s):  
Katherine M. Gaskell ◽  
Catherine Houlihan ◽  
Eleni Nastouli ◽  
Anna M. Checkley
Keyword(s):  
United Kingdom ◽  
Zika Virus ◽  
Virus Detection ◽  
The United Kingdom

scholarly journals Biological Risk Assessment: Zika Virus Detection at the Research Institute for Tropical Medicine

2016 ◽  
Vol 1 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Plebeian Medina ◽  
◽  
Catherine Calzado ◽  
Rex Centeno ◽  
Amado III Tandoc ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Zika Virus ◽  
Virus Detection ◽  
Tropical Medicine ◽  
Biological Risk ◽  
Research Institute
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