Microfabricated Nano-Gap Tunneling Current Zika Virus Sensors With Single Virus Detection Capabilities

2020 ◽  
Vol 20 (15) ◽  
pp. 8597-8603 ◽  
Author(s):  
Subhashish Dolai ◽  
Massood Tabib-Azar
The Analyst ◽  
2021 ◽  
Author(s):  
Hui-Jun Zhang ◽  
Jin Zhu ◽  
Ning Bao ◽  
Shou-Nian Ding

The mechanism of enhanced ECL of MPA@CdS QDs by EDC activation was investigated, and a sandwich-type ECL immunosensor has been designed for Zika virus detection.


2018 ◽  
Vol 113 (3) ◽  
pp. 032101 ◽  
Author(s):  
Jiancheng Yang ◽  
Patrick Carey ◽  
Fan Ren ◽  
Michael A. Mastro ◽  
Kimberly Beers ◽  
...  

2021 ◽  
Vol 31 (4) ◽  
pp. 18-27
Author(s):  
Do Thi Quynh Nga ◽  
Ha Thi Phuong Mai ◽  
Tran Thi Hai Au ◽  
Vu Thi Kim Lien ◽  
Vu Thi Bich Hau ◽  
...  

Armored RNA (AR) is a good candidate for creating nuclease-resistant RNA positive controls in the nucleic acid - based assay for RNA viruses. To simplify the production and purifcation of armored RNA, a single plasmid double – expressing His6-tag system was designed. His-tag armored RNA particles were purifed using his-tag affnity. A genomic fragment of the zika virus consisting of the encoding sequences of flavi-M, flavi-E-C protein targeted for zika virus was selected to prepare a positive control. In this study, we have successfully produced His-taged MS2- phage like particles carrying specifc genomic regions (M and E genes) to monitor the procedures of real-time Reverse transcription-PCR for Zika virus detection in one plasmid double expression. AR-ZIKA is completely resistant to DNase and RNase, stable in normal human EDTA plasma at room temperature for at least 60 and 15 days at 40C and room temperature respectively.


The Analyst ◽  
2021 ◽  
Author(s):  
Lai-Di Xu ◽  
Feng-Luan Du ◽  
Jin Zhu ◽  
Shou-Nian Ding

Fluorescent SiO2 colloids prepared from silanized CDs embedded in silica spheres were integrated with an immunochromatographic platform for Zika virus detection.


2019 ◽  
Vol 12 (5) ◽  
pp. 601-604 ◽  
Author(s):  
Zohaib Khurshid ◽  
Muhammad Zafar ◽  
Erum Khan ◽  
Maria Mali ◽  
Muhammad Latif

2019 ◽  
Vol 141 ◽  
pp. 111351 ◽  
Author(s):  
Marines Steinmetz ◽  
Dhésmon Lima ◽  
Adriano Gonçalves Viana ◽  
Sérgio Toshio Fujiwara ◽  
Christiana Andrade Pessôa ◽  
...  

2017 ◽  
Vol 23 (1) ◽  
pp. 137-139 ◽  
Author(s):  
Katherine M. Gaskell ◽  
Catherine Houlihan ◽  
Eleni Nastouli ◽  
Anna M. Checkley

2016 ◽  
Vol 1 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Plebeian Medina ◽  
◽  
Catherine Calzado ◽  
Rex Centeno ◽  
Amado III Tandoc ◽  
...  

1989 ◽  
Vol 21 (3) ◽  
pp. 147-154 ◽  
Author(s):  
Charles P. Gerba ◽  
Aaron B. Margolin ◽  
Martinez J. Hewlett

Gene probes offer a rapid and sensitive method for the detection of viruses in water and other environmental samples. Gene probes are small strands of nucleic acid labeled with radioactive or nonradioactive compounds for their detection. The target organism is identified by the hybridization of the probe to the organism's nucleic acid. Nucleic acid probes are at least 1000-fold more sensitive than serological tests such as enzyme-linked-immunoassay and do not first require cultivation of the virus for detection. Gene probes have been developed for organisms that do not grow in cell culture, and probes have been constructed for most of the major groups of enteric viruses. Gene probes have been applied to the detection of enteric viruses in water, marine sediment and shellfish. Radioactively labeled probes can detect as little as 1-10 infectious units of virus within 48 hours. A current disadvantage of probes is that they cannot determine the infectivity of the viruses; however, they can be used to quickly determine the growth of viruses in cell culture. Further development of nonradioactive probes should place virus detection capabilities into the hands of most water quality laboratories.


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