Water-soluble fullerene-based nanostructures with promising antiviral and myogenic activity

2020 ◽  
Vol 56 (70) ◽  
pp. 10203-10206
Author(s):  
Olga A. Kraevaya ◽  
Artem V. Novikov ◽  
Alexander F. Shestakov ◽  
Elizaveta S. Ershova ◽  
Ekaterina A. Savinova ◽  
...  
Keyword(s):  
Stem Cells ◽  
Phosphonic Acid ◽  
Myogenic Differentiation ◽  
Water Soluble ◽  
Fullerene Derivative ◽  
Myogenic Activity

A fullerene derivative with phosphonic acid residues demonstrated promising activity against ten different viruses and the ability to promote myogenic differentiation of stem cells.

Determination of Glutamate and GABA Released by Mouse Embryonic Stem Cells Using HILIC-ESI-MS/MS

2020 ◽  
Vol 10 (2) ◽  
pp. 122-129
Author(s):  
Haoyu Lv ◽  
Yabin Tang ◽  
Fan Sun ◽  
Shimin An ◽  
Xinjie Yang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Reversed Phase ◽  
Water Soluble ◽  
Substitution Matrix ◽  
Neural Cells ◽  
Esi Ms

Background:In recent years, more and more researches have shown that neurotransmitters can also be synthesized and released by peripheral non-neural cells. However, specificity and high sensitivity detection means were required for confirming ESCs autocrine glutamate and γ - aminobutyric acid (GABA). Glutamate and GABA are water-soluble and polar compounds which cannot be retained on a reversed phase C18 column, and their contents are often at a trace level. On the other hand, the biological matrix such as cell culture fluid contains a large number of amino acids, vitamins, carbohydrates, inorganic ions and other substances. Therefore, the main problem is the selection of the chromatographic column to avoid matrix interference.Objective:To establish a rapid and reliable method for the simultaneous determination of glutamate and GABA released from embryonic stem cells based on analytical chemistry.Methods:Glutamate and GABA released from mouse embryonic stem cells were determined on the basis of hydrophilic interaction chromatography coupled with electrospray ionization tandem Mass Spectrometry (HILIC- ESI- MS/MS), using isotope internal standards and substitution matrix method.Results:Undifferentiated embryonic stem cells autocrine glutamate and GABA and will reach releasing- reuptacking dynamic equilibriums at different time points. In contrast, neither glutamate nor GABA releasing could be detected from the MEFs, indicating the specificity release of the mESCs in the applied analytic method.Conclusion:A novel, simple, sensitive, selective and quantitative method was developed for determination of the glutamate and GABA from mouse embryonic stem cells.

scholarly journals Influence of the Active Layer Structure on the Photovoltaic Performance of Water-Soluble Polythiophene-Based Solar Cells

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1640
Author(s):  
Massimiliano Lanzi ◽  
Debora Quadretti ◽  
Martina Marinelli ◽  
Yasamin Ziai ◽  
Elisabetta Salatelli ◽  
...  
Keyword(s):  
Solar Cells ◽  
Layer Structure ◽  
Photovoltaic Performance ◽  
Phase Segregation ◽  
Water Soluble ◽  
Polymerization Reaction ◽  
Fullerene Derivative ◽  
C60 Fullerene ◽  
Photovoltaic Efficiency ◽  
Single Material

A new side-chain C60-fullerene functionalized thiophene copolymer bearing tributylphosphine-substituted hexylic lateral groups was successfully synthesized by means of a fast and effective post-polymerization reaction on a regioregular ω-alkylbrominated polymeric precursor. The growth of the polymeric intermediate was followed by NMR spectrometry in order to determine the most convenient reaction time. The obtained copolymer was soluble in water and polar solvents and was used as a photoactive layer in single-material organic photovoltaic (OPV) solar cells. The copolymer photovoltaic efficiency was compared with that of an OPV cell containing a water-soluble polythiophenic homopolymer, functionalized with the same tributylphosphine-substituted hexylic side chains, in a blend with a water-soluble C60-fullerene derivative. The use of a water-soluble double-cable copolymer made it possible to enhance the control on the nanomorphology of the active blend, thus reducing phase-segregation phenomena, as well as the macroscale separation between the electron acceptor and donor components. Indeed, the power conversion efficiency of OPV cells based on a single material was higher than that obtained with the classical architecture, involving the presence of two distinct ED and EA materials (PCE: 3.11% vs. 2.29%, respectively). Moreover, the synthetic procedure adopted to obtain single material-based cells is more straightforward and easier than that used for the preparation of the homopolymer-based BHJ solar cell, thus making it possible to completely avoid the long synthetic pathway which is required to prepare water-soluble fullerene derivatives.

A Generic Micropatterning Platform to Direct Human Mesenchymal Stem Cells from Different Origins Towards Myogenic Differentiation

2013 ◽  
Vol 13 (6) ◽  
pp. 799-807 ◽  
Author(s):  
Ting Yu ◽  
Chee Kai Chua ◽  
Chor Yong Tay ◽  
Feng Wen ◽  
Haiyang Yu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Different Origins

MYOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS IN VITRO AND LONG-TERM SURVIVAL IN A NUDE RAT MODEL FOR URINARY INCONTINENCE

2009 ◽  
Vol 181 (4) ◽  
pp. 681
Author(s):  
Gerhard Feil ◽  
Adriana Drost ◽  
Simon Baumann ◽  
Jochen Schäfer ◽  
Sibylle Weng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Urinary Incontinence ◽  
Rat Model ◽  
Myogenic Differentiation ◽  
Human Mesenchymal Stem Cells ◽  
Term Survival ◽  
Nude Rat

Nanofiber-Based in Vitro System for High Myogenic Differentiation of Human Embryonic Stem Cells

2013 ◽  
Vol 14 (12) ◽  
pp. 4207-4216 ◽  
Author(s):  
Matthew Leung ◽  
Ashleigh Cooper ◽  
Soumen Jana ◽  
Ching-Ting Tsao ◽  
Timothy A. Petrie ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Myogenic Differentiation ◽  
Embryonic Stem ◽  
In Vitro System

scholarly journals Magnetofection Mediated Transient NANOG Overexpression Enhances Proliferation and Myogenic Differentiation of Human Hair Follicle Derived Mesenchymal Stem Cells

2015 ◽  
Vol 26 (7) ◽  
pp. 1314-1327 ◽  
Author(s):  
Seoyoung Son ◽  
Mao-Shih Liang ◽  
Pedro Lei ◽  
Xiaozheng Xue ◽  
Edward P. Furlani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Human Hair ◽  
Myogenic Differentiation ◽  
Human Hair Follicle

Interactions of a Water-Soluble Fullerene Derivative with Amyloid-β Protofibrils: Dynamics, Binding Mechanism, and the Resulting Salt-Bridge Disruption

2014 ◽  
Vol 118 (24) ◽  
pp. 6733-6741 ◽  
Author(s):  
Xiaoying Zhou ◽  
Wenhui Xi ◽  
Yin Luo ◽  
Siqin Cao ◽  
Guanghong Wei
Keyword(s):  
Amyloid Β ◽  
Salt Bridge ◽  
Water Soluble ◽  
Fullerene Derivative ◽  
Binding Mechanism

Genotoxic Effect of a New Water-Soluble Fullerene Derivative C70 and its Effect on the Transcriptional Activity of Genes that Regulate the Cell Cycle, DNA Repair and Apoptosis

2021 ◽  
Vol 1031 ◽  
pp. 222-227
Author(s):  
Ekaterina A. Savinova ◽  
Elizaveta S. Ershova ◽  
Olga A. Kraevaya ◽  
Pavel A. Troshin ◽  
S.V. Kostyuk
Keyword(s):  
Cell Cycle ◽  
Transcriptional Activity ◽  
Water Soluble ◽  
Lung Fibroblasts ◽  
Fullerene Derivative ◽  
Embryonic Lung ◽  
Dna Breaks ◽  
Human Embryonic Lung ◽  
Fullerene Derivatives ◽  
Cellular Dna

It is important to take into consideration the new fullerene derivatives genotoxicity. In the present is study, we analyzed the new water-soluble fullerene C70 (F350) effects on the human embryonic lung fibroblasts (HELF) oxidative damage and DNA breaks. We found that the studied compound causes cellular DNA damage and affects the transcriptional activity of cell cycle and cell apoptosis regulating genes.

scholarly journals Small activating RNA induces myogenic differentiation of rat adipose-derived stem cells by upregulating MyoD

2015 ◽  
Vol 41 (4) ◽  
pp. 764-772 ◽  
Author(s):  
Chenghe Wang ◽  
Zhong Chen ◽  
Jia Wu ◽  
Yan Zhang ◽  
Jia Hu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Myogenic Differentiation ◽  
Adipose Derived Stem Cells

Differential Effect of Water-Soluble Chitin on Collagen Synthesis of Human Bone Marrow Stem Cells and Human Periodontal Ligament Stem Cells

2015 ◽  
Vol 21 (3-4) ◽  
pp. 451-462 ◽  
Author(s):  
So-Yon Park ◽  
Jung-Chul Park ◽  
Min-Soo Kim ◽  
Sung-Eun Lee ◽  
Ki-Joon Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Periodontal Ligament ◽  
Differential Effect ◽  
Collagen Synthesis ◽  
Bone Marrow Stem Cells ◽  
Water Soluble ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Periodontal Ligament Stem Cells
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