Interactions of a Water-Soluble Fullerene Derivative with Amyloid-β Protofibrils: Dynamics, Binding Mechanism, and the Resulting Salt-Bridge Disruption

2014 ◽  
Vol 118 (24) ◽  
pp. 6733-6741 ◽  
Author(s):  
Xiaoying Zhou ◽  
Wenhui Xi ◽  
Yin Luo ◽  
Siqin Cao ◽  
Guanghong Wei
Keyword(s):  
Amyloid Β ◽  
Salt Bridge ◽  
Water Soluble ◽  
Fullerene Derivative ◽  
Binding Mechanism

scholarly journals Influence of the Active Layer Structure on the Photovoltaic Performance of Water-Soluble Polythiophene-Based Solar Cells

Polymers ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1640
Author(s):  
Massimiliano Lanzi ◽  
Debora Quadretti ◽  
Martina Marinelli ◽  
Yasamin Ziai ◽  
Elisabetta Salatelli ◽  
...  
Keyword(s):  
Solar Cells ◽  
Layer Structure ◽  
Photovoltaic Performance ◽  
Phase Segregation ◽  
Water Soluble ◽  
Polymerization Reaction ◽  
Fullerene Derivative ◽  
C60 Fullerene ◽  
Photovoltaic Efficiency ◽  
Single Material

A new side-chain C60-fullerene functionalized thiophene copolymer bearing tributylphosphine-substituted hexylic lateral groups was successfully synthesized by means of a fast and effective post-polymerization reaction on a regioregular ω-alkylbrominated polymeric precursor. The growth of the polymeric intermediate was followed by NMR spectrometry in order to determine the most convenient reaction time. The obtained copolymer was soluble in water and polar solvents and was used as a photoactive layer in single-material organic photovoltaic (OPV) solar cells. The copolymer photovoltaic efficiency was compared with that of an OPV cell containing a water-soluble polythiophenic homopolymer, functionalized with the same tributylphosphine-substituted hexylic side chains, in a blend with a water-soluble C60-fullerene derivative. The use of a water-soluble double-cable copolymer made it possible to enhance the control on the nanomorphology of the active blend, thus reducing phase-segregation phenomena, as well as the macroscale separation between the electron acceptor and donor components. Indeed, the power conversion efficiency of OPV cells based on a single material was higher than that obtained with the classical architecture, involving the presence of two distinct ED and EA materials (PCE: 3.11% vs. 2.29%, respectively). Moreover, the synthetic procedure adopted to obtain single material-based cells is more straightforward and easier than that used for the preparation of the homopolymer-based BHJ solar cell, thus making it possible to completely avoid the long synthetic pathway which is required to prepare water-soluble fullerene derivatives.

scholarly journals Screening Assay for Small-Molecule Inhibitors of Synaptojanin 1, a Synaptic Phosphoinositide Phosphatase

2013 ◽  
Vol 19 (4) ◽  
pp. 585-594 ◽  
Author(s):  
Laura Beth J. McIntire ◽  
Kyu-In Lee ◽  
Belle Chang-Ileto ◽  
Gilbert Di Paolo ◽  
Tae-Wan Kim
Keyword(s):  
Amyloid Β ◽  
Receptor Trafficking ◽  
Water Soluble ◽  
Amyloid Β Peptide ◽  
Screening Assay ◽  
Vesicle Recycling ◽  
Lipid Phosphatase ◽  
Phosphoinositide Phosphatase ◽  
Synaptojanin 1 ◽  
Behavioral Impairments

Elevation of amyloid β-peptide (Aβ) is critically associated with Alzheimer disease (AD) pathogenesis. Aβ-induced synaptic abnormalities, including altered receptor trafficking and synapse loss, have been linked to cognitive deficits in AD. Recent work implicates a lipid critical for neuronal function, phosphatidylinositol-4,5-bisphosphate [PI(4,5)P2], in Aβ-induced synaptic and behavioral impairments. Synaptojanin 1 (Synj1), a lipid phosphatase mediating the breakdown of PI(4,5)P2, has been shown to play a role in synaptic vesicle recycling and receptor trafficking in neurons. Heterozygous deletion of Synj1 protected neurons from Aβ-induced synaptic loss and restored learning and memory in a mouse model of AD. Thus, inhibition of Synj1 may ameliorate Aβ-associated impairments, suggesting Synj1 as a potential therapeutic target. To this end, we developed a screening assay for Synj1 based on detection of inorganic phosphate liberation from a water-soluble, short-chain PI(4,5)P2. The assay displayed saturable kinetics and detected Synj1’s substrate preference for PI(4,5)P2 over PI(3,4,5)P3. The assay will enable identification of novel Synj1 inhibitors that have potential utility as chemical probes to dissect the cellular role of Synj1 as well as potential to prevent or reverse AD-associated synaptic abnormalities.

scholarly journals NMR structure of the water soluble Aβ17–34 peptide

2014 ◽  
Vol 34 (6) ◽  
Author(s):  
Genadiy Fonar ◽  
Abraham O. Samson
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Nicotinic Acetylcholine Receptors ◽  
Neurodegenerative Disorder ◽  
Amyloid Β ◽  
Helical Structure ◽  
Nmr Structure ◽  
Water Soluble ◽  
Helical Conformation ◽  
Aβ Amyloid

Alzheimer's disease is the most common neurodegenerative disorder in the world. Its most significant symptoms are memory loss and decrease in cognition. Alzheimer's disease is characterized by aggregation of two proteins in the brain namely Aβ (amyloid β) and tau. Recent evidence suggests that the interaction of soluble Aβ with nAChR (nicotinic acetylcholine receptors) contributes to disease progression. In this study, we determine the NMR structure of an Aβ17–34 peptide solubilized by the addition of two glutamic acids at each terminus. Our results indicate that the Aβ peptide adopts an α-helical structure for residues 19–26 and 28–33. The α-helical structure is broken around residues S26, N27 and K28, which form a kink in the helical conformation. This α-helix was not described earlier in an aqueous solution without organic solvents, and at physiological conditions (pH 7). These data are in agreement with Aβ adopting an α-helical conformation in the membrane before polymerizing into amyloid β-sheets and provide insight into the intermediate state of Aβ in Alzheimer's disease.

Genotoxic Effect of a New Water-Soluble Fullerene Derivative C70 and its Effect on the Transcriptional Activity of Genes that Regulate the Cell Cycle, DNA Repair and Apoptosis

2021 ◽  
Vol 1031 ◽  
pp. 222-227
Author(s):  
Ekaterina A. Savinova ◽  
Elizaveta S. Ershova ◽  
Olga A. Kraevaya ◽  
Pavel A. Troshin ◽  
S.V. Kostyuk
Keyword(s):  
Cell Cycle ◽  
Transcriptional Activity ◽  
Water Soluble ◽  
Lung Fibroblasts ◽  
Fullerene Derivative ◽  
Embryonic Lung ◽  
Dna Breaks ◽  
Human Embryonic Lung ◽  
Fullerene Derivatives ◽  
Cellular Dna

It is important to take into consideration the new fullerene derivatives genotoxicity. In the present is study, we analyzed the new water-soluble fullerene C70 (F350) effects on the human embryonic lung fibroblasts (HELF) oxidative damage and DNA breaks. We found that the studied compound causes cellular DNA damage and affects the transcriptional activity of cell cycle and cell apoptosis regulating genes.

scholarly journals Heterogeneity of water-soluble amyloid β-peptide in Alzheimer's disease and Down's syndrome brains

FEBS Letters ◽  
1997 ◽  
Vol 409 (3) ◽  
pp. 411-416 ◽  
Author(s):  
Claudio Russo ◽  
Takaomi C. Saido ◽  
Laura M. DeBusk ◽  
Massimo Tabaton ◽  
Pierluigi Gambetti ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Down's Syndrome ◽  
Amyloid Β ◽  
Down’S Syndrome ◽  
Water Soluble ◽  
Amyloid Β Peptide

scholarly journals Link between Affinity and Cu(II) Binding Sites to Amyloid-β Peptides Evaluated by a New Water-Soluble UV–Visible Ratiometric Dye with a Moderate Cu(II) Affinity

2017 ◽  
Vol 89 (3) ◽  
pp. 2155-2162 ◽  
Author(s):  
Amandine Conte-Daban ◽  
Valentina Borghesani ◽  
Stéphanie Sayen ◽  
Emmanuel Guillon ◽  
Yves Journaux ◽  
...  
Keyword(s):  
Binding Sites ◽  
Amyloid Β ◽  
Water Soluble ◽  
Uv Visible

Use of a new water-soluble Zn sensor to determine Zn affinity for the amyloid-β peptide and relevant mutants

Metallomics ◽  
2014 ◽  
Vol 6 (7) ◽  
pp. 1220 ◽  
Author(s):  
Sabrina Noël ◽  
Susana Bustos Rodriguez ◽  
Stéphanie Sayen ◽  
Emmanuel Guillon ◽  
Peter Faller ◽  
...  
Keyword(s):  
Amyloid Β ◽  
Water Soluble ◽  
Amyloid Β Peptide

Water-soluble chitosan inhibits the production of pro-inflammatory cytokine in human astrocytoma cells activated by amyloid β peptide and interleukin-1β

2002 ◽  
Vol 321 (1-2) ◽  
pp. 105-109 ◽  
Author(s):  
Mi-Sun Kim ◽  
Man-Joon Sung ◽  
Sang-Bong Seo ◽  
Su-Jin Yoo ◽  
Woon-Ki Lim ◽  
...  
Keyword(s):  
Inflammatory Cytokine ◽  
Amyloid Β ◽  
Water Soluble ◽  
Interleukin 1Β ◽  
Amyloid Β Peptide ◽  
Astrocytoma Cells ◽  
Human Astrocytoma ◽  
Human Astrocytoma Cells

scholarly journals THE A water-soluble [60]fullerene-derivative stimulates chlorophyll accumulation and has no toxic effect on Chlamydomonas reinhardtii

2019 ◽  
Author(s):  
Jakub Lang ◽  
Mariia Melnykova ◽  
Michele Catania ◽  
Alicja Inglot ◽  
Aleksandra Zyss ◽  
...  
Keyword(s):  
Growth Rate ◽  
Chlamydomonas Reinhardtii ◽  
Algal Growth ◽  
Water Soluble ◽  
Fullerene Derivative ◽  
Industrial Biotechnology ◽  
Photosynthetic Parameters ◽  
Suitable Model ◽  
Chlorophyll Accumulation ◽  
Surface Modified

Chlamydomonas reinhardtii (WT 2137) P. A. Dang. (Volvocales, Chlorophyceae) is a green microalgae serving as a suitable model in scientific research and a promising industrial biotechnology platform for production of biofuel, hydrogen and recombinant proteins. Fullerenes (C60) are allotropic carbon nanoparticles discovered in 1985 and used in biomedical studies since the early 1990s, when water solubilization methodologies were developed. Recently, surface-modified hydroxylated derivatives of fullerenes were proven to enhance algal growth and drought tolerance in plants. Here, a novel type of water-soluble [60]fullerene derivative with 12 glycine residues (GF) has been synthesized and tested for acute toxicity (up to 50 µg/ml) and as a potential biostimulant of algal growth. The effects of GF on pigment composition and growth rate of Chlamydomonas reinhardtii were systematically investigated. Our results suggest that GF was not toxic, and no negative change in the pigment content and no stress symptoms were observed. No changes in the photosynthetic parameters based on the fluorescence of chlorophyll a in Photosystem II (NPQ, Fv/Fm, Fv/F0, PI and RC/ABS) were observed. The GF had no effect on cell size and growth rate. At a concentration of 20 µg/ml, GF stimulated chlorophyll accumulation in 3-day-old cultures.

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