A hepatocyte-targeting fluorescent probe for imaging isoniazid-induced hydrazine in HepG2 cells and zebrafish

2020 ◽  
Vol 56 (91) ◽  
pp. 14183-14186
Author(s):  
Zhenbo Guo ◽  
Mei Wang ◽  
Xueyan Li ◽  
Xu Jia ◽  
Xiaoli Wang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepg2 Cells ◽  
Hydrolysis Of

A novel hepatocyte-targeting fluorescent N2H4 probe was first prepared. The probe can be used to image N2H4 produced by the hydrolysis of isoniazid in HepG2 cells and the liver of zebrafish in situ.

scholarly journals Construction of a Turn Off-On-Off Fluorescent System Based on Competitive Coordination of Cu2+between 6,7-Dihydroxycoumarin and Pyrophosphate Ion for Sensitive Assay of Pyrophosphatase Activity

2016 ◽  
Vol 2016 ◽  
pp. 1-10
Author(s):  
Lingzhi Zhao ◽  
Liu Zhao ◽  
Yanqing Miao ◽  
Chunye Liu ◽  
Chenxiao Zhang
Keyword(s):  
Fluorescent Probe ◽  
Reaction System ◽  
Water Soluble ◽  
Sensitive Assay ◽  
Optimum Conditions ◽  
Activity Assay ◽  
Competitive Coordination ◽  
Hydrolysis Of ◽  
Subsequent Addition

The detection of pyrophosphatase (PPase) activity is of great significance in diagnosing diseases and understanding the function of PPase-related biological events. This study constructed a turn off-on-off fluorescent system for PPase activity assay based on PPase-regulated competitive coordination of Cu2+between a water-soluble fluorescent probe 6,7-dihydroxycoumarin (DHC) and pyrophosphate (PPi). The probe DHC can coordinate with Cu2+and consequently display on-off type fluorescence response. Furthermore, the in situ formed nonfluorescent Cu2+-DHC complex can act as an effective off-on type fluorescent probe for sensing PPi due to the higher coordination reactivity between Cu2+and PPi than that between Cu2+and DHC. The subsequent addition of PPase to the mixture containing Cu2+, DHC, and PPi leads to the fluorescence requenching of the system again (an off state) because PPase catalyzes the hydrolysis of PPi into orthophosphate in the reaction system. Under the optimum conditions, the decrease of the fluorescence intensity of DHC-Cu2+-PPi system was linear with the increase of the PPase activity in the range from 0.1 to 0.3 U. The detection limit was down to 0.028 U PPase (S/N=3). Moreover, the as-established system was also applied to evaluate PPase inhibitor. This study offers a simple yet effective method for the detection of PPase activity.

Transmembrane gradients of free Na+ in isolated heart mitochondria estimated using a fluorescent probe

1992 ◽  
Vol 262 (4) ◽  
pp. C1047-C1055 ◽  
Author(s):  
D. W. Jung ◽  
L. M. Apel ◽  
G. P. Brierley
Keyword(s):  
Fluorescent Probe ◽  
Free Acid ◽  
Isolated Heart ◽  
Heart Mitochondria ◽  
Excitation Spectra ◽  
Acetoxymethyl Ester ◽  
The Matrix ◽  
Isolated Pig Heart ◽  
Hydrolysis Of

The concentration of free Na+ in the matrix of isolated pig heart mitochondria has been monitored using the fluorescent probe sodium-binding benzofuran isophthalate (SBFI) developed by Minta and Tsien (J. Biol. Chem. 264: 19449-19457, 1989). SBFI was sequestered in the matrix by hydrolysis of the permeant acetoxymethyl ester. The sequestered probe showed altered quantum efficiency and excitation spectra in the presence and absence of Na+ when compared with SBFI free acid in solution. Fluorescence was calibrated in situ by using ionophores to equilibrate matrix [Na+] with external [Na+]. SBFI fluorescence showed that matrix [Na+] increased linearly as external [Na+] was increased to 95 mM in the presence or absence of respiration. Respiring mitochondria maintained a Na+ gradient (Na+ out greater than Na+in) of approximately 8.0. The corresponding gradient in nonrespiring mitochondria was approximately 2.0. The Na+ gradient was nearly equivalent to the H+ gradient in the presence or absence of respiration. The uptake of Pi by respiring mitochondria decreased matrix pH and increased matrix [Na+]. It is concluded that isolated mitochondria maintain a Na+ gradient across the inner membrane as a result of the activity of the endogenous Na(+)-H+ antiport.

INDIREKTE PAPIERCHROMATOGRAPHISCHE METHODE ZUR BESTIMMUNG DER HARNOESTROGENE

1961 ◽  
Vol 38 (4) ◽  
pp. 545-562 ◽  
Author(s):  
L. Kecskés ◽  
F. Mutschler ◽  
I. Glós ◽  
E. Thán ◽  
I. Farkas ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Granulosa Cell ◽  
Iron Content ◽  
List Type ◽  
Granulosa Cell Tumour ◽  
Hydrolysis Of ◽  
Phenol Fraction ◽  
Qualitative Determination

ABSTRACT 1. An indirect paperchromatographic method is described for separating urinary oestrogens; this consists of the following steps: acidic hydrolysis, extraction with ether, dissociation of phenol-fractions with partition between the solvents. Previous purification of phenol fraction with the aid of paperchromatography. The elution of oestrogen containing fractions is followed by acetylation. Oestrogen acetate is isolated by re-chromatography. The chromatogram was developed after hydrolysis of the oestrogens 'in situ' on the paper. The quantity of oestrogens was determined indirectly, by means of an iron-reaction, after the elution of the iron content of the oestrogen spot, which was developed by the Jellinek-reaction. 2. The method described above is satisfactory for determining urinary oestrogen, 17β-oestradiol and oestriol, but could include 16-epioestriol and other oestrogenic metabolites. 3. The sensitivity of the method is 1.3–1.6 μg/24 hours. 4. The quantitative and qualitative determination of urinary oestrogens with the above mentioned method was performed in 50 pregnant and 9 non pregnant women, and also in 2 patients with granulosa cell tumour.

In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose

2013 ◽  
Vol 85 (17) ◽  
pp. 8121-8126 ◽  
Author(s):  
Britta Opitz ◽  
Andreas Prediger ◽  
Christian Lüder ◽  
Marrit Eckstein ◽  
Lutz Hilterhaus ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Hydrolysis Of Cellulose ◽  
Hydrolysis Of Cellulose ◽  
Hydrolysis Of

Visualizing hydrogen sulfide in HepG2 cells exposed to OGD/R and in the liver of zebrafish with a hepatocyte-targeting fluorescent probe

2021 ◽  
Vol 188 ◽  
pp. 109151
Author(s):  
Meng Yao ◽  
Xu Jia ◽  
Mei Wang ◽  
Xin Li ◽  
Xiaoli Wang ◽  
...  
Keyword(s):  
Hydrogen Sulfide ◽  
Fluorescent Probe ◽  
Hepg2 Cells

Turn-on fluorescent probe for dopamine detection in solutions and live cells based on in situ formation of aminosilane-functionalized carbon dots

2021 ◽  
Vol 1157 ◽  
pp. 338394
Author(s):  
Xiao-Yue Tang ◽  
Yi-Ming Liu ◽  
Xiao-Lin Bai ◽  
Hao Yuan ◽  
Yi-Kao Hu ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Carbon Dots ◽  
Live Cells ◽  
Turn On ◽  
Dopamine Detection ◽  
In Situ Formation

scholarly journals Permeability of muscle capillaries to small heme-peptides. Evidence for the existence of patent transendothelial channels.

1975 ◽  
Vol 64 (3) ◽  
pp. 586-607 ◽  
Author(s):  
N Simionescu ◽  
M Siminoescu ◽  
G E Palade
Keyword(s):  
Plasma Proteins ◽  
Intercellular Junctions ◽  
Rat Diaphragm ◽  
Enzymic Hydrolysis ◽  
Graphic Analysis ◽  
Heme Peptides ◽  
Future Work ◽  
Hydrolysis Of ◽  
Muscle Capillaries

Two heme-peptides (HP) of about 20-A diameter (heme-undecapeptide [H11P], mol wt approximately 1900 and heme-octapeptide [H8P], mol wt approximately 1550), obtained by enzymic hydrolysis of cytochrome c, were sued as probe molecules in muscle capillaries (rat diaphragm). They were localized in situ by a perixidase reaction, enhanced by the addition of imidazole to the incubation medium. Chromatography of plasma samples showed that HPs circulate predominantly as monomers for the duration of the experiments and are bound by aldehyde fixatives to plasma proteins to the extent of approximately 50% (H8P) to approximately 95% (H11P). Both tracers cross the endothelium primarily via plasmalemmal vesicles which become progressively labeled (by reaction product) from the blood front to the tissue front of the endothelium, in three successive resolvable phases. By the end of each phase the extent of labeling reaches greater than 90% of the corresponding vesicle population. Labeled vesicles appear as either isolated units or chains which form patent channels across the endothelium. The patency of these channels was checked by specimen tilting and graphic analysis of their images. No evidence was found for early or preferential marking of the intercellular junctions and spaces by reaction product. It is concluded that the channels are the most likely candidate for structural equivalents of the small pores of the capillary wall since they are continuous, water-filled passages, and are provided with one or more strictures of less than 100 A. Their frequency remains to be established by future work.

I2 catalyzed access of spiro[indoline-3,4′-pyridine] appended amine dyad: new ON–OFF chemosensors for Cu2+ and imaging in living cells

2018 ◽  
Vol 16 (2) ◽  
pp. 302-315 ◽  
Author(s):  
Animesh Mondal ◽  
Barnali Naskar ◽  
Sanchita Goswami ◽  
Chandraday Prodhan ◽  
Keya Chaudhuri ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Hepg2 Cells ◽  
Cell Imaging ◽  
Colorimetric Detection ◽  
Living Cells ◽  
Fluorescent Cell

An efficient, easily tuneable route to construct a structurally diverse organic fluorescent probe and its applications towards the colorimetric detection of Cu2+ ions and in vitro fluorescent cell imaging of Cu2+ in HepG2 cells.

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