Revisiting staining of biological samples for electron microscopy: perspectives for recent research

2021 ◽  
Author(s):  
Maren T. Kuchenbrod ◽  
Ulrich S. Schubert ◽  
Rainer Heintzmann ◽  
Stephanie Hoeppener
Keyword(s):  
Electron Microscopy ◽  
Biological Samples ◽  
Active Sites ◽  
In Cells

This review revisits staining protocols for electron microscopy focussing on the visualization of active sites, i.e. enzymes, metabolites or proteins, in cells and tissues, which were never established as standard protocols in electron microscopy.

scholarly journals Protocol for preparation of heterogeneous biological samples for 3D electron microscopy: a case study for insects

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Alexey A. Polilov ◽  
Anastasia A. Makarova ◽  
Song Pang ◽  
C. Shan Xu ◽  
Harald Hess
Keyword(s):  
Electron Microscopy ◽  
Biological Samples ◽  
Focused Ion Beam ◽  
Ion Beam ◽  
Three Dimensional ◽  
Entire Volume ◽  
Simple Protocol ◽  
3D Electron Microscopy ◽  
3D Em

AbstractModern morphological and structural studies are coming to a new level by incorporating the latest methods of three-dimensional electron microscopy (3D-EM). One of the key problems for the wide usage of these methods is posed by difficulties with sample preparation, since the methods work poorly with heterogeneous (consisting of tissues different in structure and in chemical composition) samples and require expensive equipment and usually much time. We have developed a simple protocol allows preparing heterogeneous biological samples suitable for 3D-EM in a laboratory that has a standard supply of equipment and reagents for electron microscopy. This protocol, combined with focused ion-beam scanning electron microscopy, makes it possible to study 3D ultrastructure of complex biological samples, e.g., whole insect heads, over their entire volume at the cellular and subcellular levels. The protocol provides new opportunities for many areas of study, including connectomics.

scholarly journals Facile Synthesis of Carbon Cloth Supported Cobalt Carbonate Hydroxide Hydrate Nanoarrays for Highly Efficient Oxygen Evolution Reaction

2021 ◽  
Vol 9 ◽  
Author(s):  
Yubing Yan
Keyword(s):  
Electron Microscopy ◽  
Oxygen Evolution ◽  
Current Density ◽  
Oxygen Evolution Reaction ◽  
Active Sites ◽  
Carbon Cloth ◽  
High Porosity ◽  
Cobalt Carbonate ◽  
Carbonate Hydroxide ◽  
Hydroxide Hydrate

Developing efficient and low-cost replacements for noble metals as electrocatalysts for the oxygen evolution reaction (OER) remain a great challenge. Herein, we report a needle-like cobalt carbonate hydroxide hydrate (Co(CO3)0.5OH·0.11H2O) nanoarrays, which in situ grown on the surface of carbon cloth through a facile one-step hydrothermal method. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) characterizations demonstrate that the Co(CO3)0.5OH nanoarrays with high porosity is composed of numerous one-dimensional (1D) nanoneedles. Owing to unique needle-like array structure and abundant exposed active sites, the Co(CO3)0.5OH@CC only requires 317 mV of overpotential to reach a current density of 10 mA cm−2, which is much lower than those of Co(OH)2@CC (378 mV), CoCO3@CC (465 mV) and RuO2@CC (380 mV). For the stability, there is no significant attenuation of current density after continuous operation 27 h. This work paves a facile way to the design and construction of electrocatalysts for the OER.

Melanogenesis in amphibians

Development ◽  
1970 ◽  
Vol 24 (2) ◽  
pp. 447-454
Author(s):  
John J. Eppig
Keyword(s):  
Electron Microscopy ◽  
Epithelial Cells ◽  
Notophthalmus Viridescens ◽  
Retinal Pigmented Epithelium ◽  
Pigmented Epithelium ◽  
In Cells

Electron microscopy of 11-day-old Notophthalmus viridescens retinal pigmented epithelium reveals particulate premelanosomes which are identical to the melanosomes found in the oocyte. These organelles, when found in the pigmented epithelium, are called premelanosomes because they undergo further maturation to form relatively homogeneous, spherical melanosomes. At this stage, oocyte melanosomes found in cells other than melanocytes have not undergone this subsequent maturation. Elongated melanosomes which develop from fibrillar premelanosomes are also found in the pigmented epithelial cells. Treatment with phenylthiourea blocks the maturation of both the fibrillar and particulate premelanosomes.

Energy-Filtered Imaging of Zirconia Pillars in Montmorillonite

1998 ◽  
Vol 4 (S2) ◽  
pp. 744-745
Author(s):  
P. A. Crazier ◽  
M. Pan ◽  
C. Bateman ◽  
J. J. Alcaraz ◽  
J. S. Holmgren
Keyword(s):  
Electron Microscopy ◽  
Elemental Composition ◽  
Active Sites ◽  
Analytical Electron Microscopy ◽  
Montmorillonite Clay ◽  
Considerable Effort ◽  
Pillared Montmorillonite ◽  
Trade Name ◽  
Analytical Electron ◽  
Fine Tune

The ability to vary the plane spacing in different clays is becoming increasingly important in catalysis and other technological applications [Baker and Murrell, 1990]. Considerable effort is now being devoted to understanding the mechanisms involved in pillaring such materials with a view to being able to fine-tune parameters such as the plane spacing and active sites for the application of interest. We have been able to use analytical electron microscopy to directly image the pillars in zirconia pillared montmorillonite. By using a combination of TEM techniques, we are able to correlate morphological information with elemental composition and conclusively identify the location of the zirconia pillars.A sample of natural montmorillonite clay, trade name HPM-20, was obtained from American Colloid and used as received. Zirconia pillared clays were prepared following published procedures [Bartley, 1988]. The Z1O2 pillars were prepared by using hydroxyzirconium cations produced by the solvation of zironyl chloride.

Electron microscopy of sowthistle yellow vein virus in cells of sowthistle plants

Virology ◽  
1972 ◽  
Vol 48 (3) ◽  
pp. 739-748 ◽  
Author(s):  
Peter E. Lee ◽  
D. Peters
Keyword(s):  
Electron Microscopy ◽  
Yellow Vein ◽  
In Cells

scholarly journals Correlative Light and Electron Microscopy (CLEM) Techniques for Biological Samples

2013 ◽  
Vol 19 (S2) ◽  
pp. 100-101
Author(s):  
V. Nair ◽  
F.H. Hoyt ◽  
T. Hoenen ◽  
E.R. Fischer
Keyword(s):  
Electron Microscopy ◽  
Biological Samples ◽  
Light And Electron Microscopy

Extended abstract of a paper presented at Microscopy and Microanalysis 2013 in Indianapolis, Indiana, USA, August 4 – August 8, 2013.

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