Near-infrared nanosecond-pulsed laser-activated highly efficient intracellular delivery mediated by nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles

Nanoscale ◽  
2020 ◽  
Vol 12 (22) ◽  
pp. 12057-12067 ◽  
Author(s):  
Tuhin Subhra Santra ◽  
Srabani Kar ◽  
Te-Chang Chen ◽  
Chih-Wei Chen ◽  
Jayant Borana ◽  
...  
Keyword(s):  
Cell Viability ◽  
Near Infrared ◽  
Pulsed Laser ◽  
Intracellular Delivery ◽  
High Cell ◽  
Polystyrene Nanoparticles ◽  
Infrared Wavelength ◽  
Highly Efficient ◽  
Nanosecond Pulsed Laser

Here, an efficient intracellular delivery of molecules with high cell viability is reported using plasmonic photoporation mediated by nano-corrugated mushroom-shaped gold-coated polystyrene nanoparticles at near-infrared wavelength.

scholarly journals Efficient Intracellular Delivery of Molecules with High Cell Viability Using Nanosecond-Pulsed Laser-Activated Carbon Nanoparticles

ACS Nano ◽  
2014 ◽  
Vol 8 (3) ◽  
pp. 2889-2899 ◽  
Author(s):  
Aritra Sengupta ◽  
Sean C. Kelly ◽  
Nishant Dwivedi ◽  
Naresh Thadhani ◽  
Mark R. Prausnitz
Keyword(s):  
Activated Carbon ◽  
Cell Viability ◽  
Carbon Nanoparticles ◽  
Pulsed Laser ◽  
Intracellular Delivery ◽  
High Cell ◽  
Nanosecond Pulsed Laser

Nano-localized single-cell nano-electroporation

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4194-4204
Author(s):  
Tuhin Subhra Santra ◽  
Srabani Kar ◽  
Hwan-You Chang ◽  
Fan-Gang Tseng
Keyword(s):  
Cell Viability ◽  
Single Cell ◽  
Intracellular Delivery ◽  
High Cell ◽  
Highly Efficient

We demonstrated nano-electroporation technique to create transient nano-holes at single or multiple nano-localized positions of a single-cell for a highly efficient intracellular delivery with high cell viability.

Growth and Properties of Pulsed Laser Deposited K3Li2Nb5O15 Thin Films

2007 ◽  
Vol 351 ◽  
pp. 184-188 ◽  
Author(s):  
Zu Sheng Zhan ◽  
Yan Sheng Gong ◽  
Qiang Shen ◽  
Lian Meng Zhang
Keyword(s):  
Near Infrared ◽  
Ambient Pressure ◽  
Tungsten Bronze ◽  
Pulsed Laser ◽  
Laser Deposition ◽  
Optical Studies ◽  
Tetragonal Tungsten Bronze ◽  
Infrared Wavelength ◽  
Optimum Parameters ◽  
Substrate Temperatures

Potassium lithium niobate (KLN: K3Li2Nb5O15) films have been deposited on quartz glass by Pulsed laser deposition (PLD) technique using a stoichiometric KLN target as starting materials. By investigating the effects of both the oxygen pressure and the substrate temperature on the structure of KLN films, optimum parameters have been identified for the growth of high-quality KLN films. At 10Pa oxygen ambient pressure, tetragonal tungsten-bronze-type structure of KLN films with (310) preferred orientation can be achieved at substrate temperatures in the range of 700-800°C. Optical studies indicate that the films are highly transparent in the visible-near-infrared wavelength range.

scholarly journals Luminescent silicon nanocrystals produced by near-infrared nanosecond pulsed laser ablation in water

2014 ◽  
Vol 302 ◽  
pp. 62-65 ◽  
Author(s):  
L. Vaccaro ◽  
L. Sciortino ◽  
F. Messina ◽  
G. Buscarino ◽  
S. Agnello ◽  
...  
Keyword(s):  
Laser Ablation ◽  
Silicon Nanocrystals ◽  
Near Infrared ◽  
Pulsed Laser ◽  
Pulsed Laser Ablation ◽  
Nanosecond Pulsed Laser

scholarly journals Photo-Induced Cell Damage Analysis for Single- and Multifocus Coherent Anti-Stokes Raman Scattering Microscopy

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Takeo Minamikawa ◽  
Yoshinori Murakami ◽  
Naokazu Matsumura ◽  
Hirohiko Niioka ◽  
Shuichiro Fukushima ◽  
...  
Keyword(s):  
Raman Scattering ◽  
Cell Viability ◽  
Near Infrared ◽  
Cell Damage ◽  
Pulsed Laser ◽  
Excitation Scheme ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Anti Stokes ◽  
Photon Fluorescence

In this study, we investigated photo-induced damage to living cells during single- and multifocus excitations for coherent anti-Stokes Raman scattering (CARS) imaging. A near-infrared pulsed laser (709 nm) was used to induce cell damage. We compared the photo-induced cell damage in the single- and the multifocus excitation schemes with the condition to obtain the same CARS signal in the same frame rate. For the evaluation of cell viability, we employed 4′,6-diamidino-2-phenylindole (DAPI) fluorophores that predominantly stained the damaged cells. One- and two-photon fluorescence of DAPI fluorophores were, respectively, excited by an ultraviolet light source and the same near-infrared light source and were monitored to evaluate the cell viability during near-infrared pulsed laser irradiation. We found lower uptake of DAPI fluorophores into HeLa cells during the multifocus excitation compared with the single-focus excitation scheme in both the one- and the two-photon fluorescence examinations. This indicates a reduction of photo-induced cell damage in the multifocus excitation. Our findings suggested that the multifocus excitation scheme is expected to be suitable for CARS microscopy in terms of minimal invasiveness.

scholarly journals Highly transparent conductive AZO/Zr50Cu50/AZO films in wide range of visible and near infrared wavelength grown by pulsed laser deposition

2017 ◽  
Vol 7 ◽  
pp. 910-913 ◽  
Author(s):  
Jingyun Cheng ◽  
Guohua Cao ◽  
Haitao Zong ◽  
Chaoyang Kang ◽  
Erguang Jia ◽  
...  
Keyword(s):  
Pulsed Laser Deposition ◽  
Near Infrared ◽  
Pulsed Laser ◽  
Laser Deposition ◽  
Infrared Wavelength ◽  
Wide Range

Effect of process parameters in nanosecond pulsed laser micromachining of PMMA-based microchannels at near-infrared and ultraviolet wavelengths

2012 ◽  
Vol 67 (5-8) ◽  
pp. 1651-1664 ◽  
Author(s):  
Daniel Teixidor ◽  
Francisco Orozco ◽  
Thanongsak Thepsonthi ◽  
Joaquim Ciurana ◽  
Ciro A. Rodríguez ◽  
...  
Keyword(s):  
Process Parameters ◽  
Near Infrared ◽  
Pulsed Laser ◽  
Laser Micromachining ◽  
Nanosecond Pulsed Laser
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