scholarly journals Studies of fibronectin matrices in living cells with fluoresceinated gelatin.

1980 ◽  
Vol 87 (1) ◽  
pp. 14-22 ◽  
Author(s):  
P Hsieh ◽  
R Segal ◽  
L B Chen
Keyword(s):  
Extracellular Matrix ◽  
Chick Embryo ◽  
Cell Surface ◽  
Cell Lines ◽  
Cultured Cells ◽  
Living Cells ◽  
Low Concentration ◽  
Established Cell Lines ◽  
The Matrix ◽  
Established Cell

We have used fluorescein isosthiocyanate-conjugated gelatin (FITC-gelatin) (1 mg/ml) to localize cell surface fibronectin in unfixed live cells in cultures. FITC-gelatin stains the fibronectin matrix on primary cultures of rat and chick embryo fibroblasts as well as untransformed, established cell lines. In live cultured cells, fibronectin in many areas of the extracellular matrix is inaccessible to antibody and cannot be visualized by immunofluorescence staining. In contrast, fibronectin in these areas is fully stainable by FITC-gelatin. At a low concentration (20 micrograms/ml), FITC-gelatin stains the fibronectin matrix of primary cultured cells but not of "untransformed" established cell lines. SEM can detect only the matrix stainable with the low concentration of FITC-gelatin, such as that expressed by primary chick embryo fibroblasts. The binding of fibronectin to the extracellular matrix is very stable and FITC-gelatin remained bound to the matrix for at least 10 d in culture. Radioiodinated gelatin has been used to quantitate the level of cell surface fibronectin in living normal and transformed cells. FITC-gelatin appears to be a useful probe for studying the fibronectin of living cells in culture.

CEA expression patterns determine response and resistance to the CEA-TCB bispecific immunotherapy antibody in colorectal cancer patient derived organoids.

2019 ◽  
Vol 37 (4_suppl) ◽  
pp. 535-535
Author(s):  
Reyes Gonzalez Exposito ◽  
Maria Semmianikova ◽  
Beatrice Griffiths ◽  
Khurum Hayat Khan ◽  
Louise J Barber ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
T Cells ◽  
Cell Surface ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cell Killing ◽  
Cell Surface Expression ◽  
High Plasticity ◽  
Established Cell Lines ◽  
Established Cell

535 Background: The bispecific antibody CEA-TCB binds Carcino-Embryonic Antigen (CEA) on cancer cells and CD3 on T cells. This triggers T cell killing of colorectal cancer cell lines expressing moderate to high levels of CEA at the cell surface (Bacac, Clin Cancer Res 2016). Patient derived organoids (PDOs) may more accurately represent patient tumors than established cell lines. Yet, determinants of CEA-TCB resistance have not been studied in PDOs. Methods: PDOs were established from biopsies of eight multidrug-resistant metastatic CRCs, GFP labelled and adapted to 2D culture. Allogenic CD8 T cells and CEA-TCB or a non-targeting control antibody were added and cancer cell killing and growth were monitored for 10 days. CEA expression of PDOs was determined by FACS. Results: CRC PDOs could be categorized into three groups based on CEA cell-surface expression: CEAhigh (n = 3), CEAlow (n = 2), and CEA heterogeneous PDOs (n = 3) that stably maintained populations of both CEAhigh and CEAlow cells, which has not previously been described in CRC cell lines. Heterogeneity of cell-surface CEA expression is common in CRC cells in patients, supporting that PDOs may better represent these tumors than established cell lines. CEAhigh cells were sensitive whereas CEAlow cells showed resistance to CEA-TCB. All PDOs with heterogeneous CEA expression were resistant to CEA-TCB, suggesting that CEA-negative cells maintain cancer cell growth. Culture of FACS sorted CEAhigh and CEAlow cells from PDOs with heterogeneous CEA expression demonstrated high plasticity of CEA expression which may contribute to rapid resistance acquisition through CEA antigen loss. Conclusions: These results suggest that cell-surface CEA expression is a major determinant of CEA-TCB sensitivity and resistance in PDOs. In addition, we identified heterogeneous CEA expression in several PDOs and demonstrated that this could confer CEA-TCB resistance in vitro. These PDO models are likely to provide insights into the mechanism of CEA loss and may inform therapeutic opportunities to counter CEA-TCB resistance. RNA-sequencing and functional experiments are ongoing to investigate this and will be presented.

scholarly journals Effects of Physicochemical Properties of Polyacrylamide (PAA) and Polydimethylsiloxane (PDMS) on Cardiac Cell behavior

Soft Matter ◽  
2021 ◽  
Author(s):  
Karim Daliri ◽  
Kurt Pfannkuche ◽  
Bora Garipcan
Keyword(s):  
Cell Lines ◽  
Cultured Cells ◽  
Cell Behavior ◽  
Cardiac Cell ◽  
Established Cell Lines ◽  
The World ◽  
Established Cell ◽  
Primary Origin ◽  
Transformed Cell Lines

In vitro cell culture is commonly applied in laboratories around the world. Cultured cells are either of primary origin or established cell lines. Such transformed cell lines are increasingly replaced...

scholarly journals Demonstration of Isoantigenic Receptors on Human Epidermal Cells and Established Cell Lines

Blood ◽  
1965 ◽  
Vol 25 (1) ◽  
pp. 96-104 ◽  
Author(s):  
EDMOND J. YUNIS ◽  
JORGE J. YUNIS ◽  
K. GERHARD BRAND
Keyword(s):  
Cell Lines ◽  
Human Cell ◽  
Cultured Cells ◽  
Mouse Cell ◽  
Epidermal Cells ◽  
Established Cell Lines ◽  
Human Cell Cultures ◽  
Human Epidermal Cells ◽  
Established Cell ◽  
Cell Strains

Abstract This paper describes mixed agglutination and serum absorption experiments for the demonstration of A, B, H, M, N, D, C, E, c and e isoantigens in human epidermal cells and cultured cells. It was found that only A, B and H antigens are present on human epidermal cells and that this does not appear to be related to the secretor status of the donor. The same antigens were also examined on six different established human cell strains (HeLa, EE, ERK-1, Maben, Chang conjunctiva, Chang liver), and one established mouse cell strain (L). It was found that the H antigen persists in established human cell cultures. The M antigen which is not seen in normal epithelial cells can be demonstrated on HeLa cells by mixed agglutination reaction and by absorption experiments. Guinea pig antisera against established cell lines of human origin were found to contain a small fraction of agglutinins with H specificity, but no anti-M, anti-N or anti-Rh agglutinins.

scholarly journals Complementation between urokinase-producing and receptor-producing cells in extracellular matrix degradation.

1991 ◽  
Vol 2 (10) ◽  
pp. 793-803 ◽  
Author(s):  
P H Quax ◽  
N Pedersen ◽  
M T Masucci ◽  
E J Weening-Verhoeff ◽  
K Danø ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Cell Surface ◽  
Plasminogen Activator ◽  
Cell Lines ◽  
Phosphatidyl Inositol ◽  
Matrix Degradation ◽  
Extracellular Matrix Degradation ◽  
The Matrix ◽  
Extracellular Proteolysis

The respective roles of urokinase plasminogen activator (u-PA) and the u-PA receptor in extracellular matrix degradation was investigated. Human pro-u-PA and the human u-PA receptor were expressed independently by two different mouse LB6 cell lines. The matrix degradation capacity of these cell lines individually or in coculture was studied. Although pro-u-PA-producing cells alone degrade the matrix in the presence of plasminogen, u-PA-receptor producing cells do not. Cocultivation of a small fraction of pro-u-PA-producing cells with the receptor-producing cells increases the rate of matrix degradation at least threefold. By immunoprecipitation it was shown that cocultivation of the two cell lines increases the conversion of the inactive pro-u-PA to the active two chain u-PA. The enhancement of matrix degradation and of pro-u-PA activation requires actual binding of pro-u-PA to its receptor because it is inhibited by u-PA-receptor antagonists. The u-PA receptor must be cell associated, as binding of pro-u-PA to a receptor solubilized from the cell surface with phosphatidyl-inositol specific phospholipase C did not enhance the activation of pro-u-PA in the presence of plasminogen. The finding that activity of u-PA is enhanced when it is bound to its receptor, even when the receptor is produced by a different cell, might have important implications for the mechanisms of u-PA-induced extracellular proteolysis in vivo.

Lysis of human B-lymphocyte-derived lymphoma/leukemia cells of established cell lines by interferon-activated natural killer (NK) cells

1981 ◽  
Vol 28 (4) ◽  
pp. 459-468 ◽  
Author(s):  
Paul K. Pattengale ◽  
Magnus Gidlund ◽  
Kenneth Nilsson ◽  
Christer Sundström ◽  
Anders Örn ◽  
...  
Keyword(s):  
Nk Cells ◽  
Natural Killer ◽  
Cell Lines ◽  
B Lymphocyte ◽  
Leukemia Cells ◽  
Established Cell Lines ◽  
Established Cell

scholarly journals Growth of Nosema cuniculi in established cell lines

1975 ◽  
Vol 9 (1) ◽  
pp. 61-68 ◽  
Author(s):  
T. Waller
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Growth Patterns ◽  
Rabbit Kidney ◽  
Kidney Cells ◽  
Scale Production ◽  
Simple Method ◽  
Established Cell Lines ◽  
Established Cell ◽  
Canine Kidney

Growth patterns of Nosema cuniculi ( Encephalitozoon cuniculi) in cell cultures of bovine kidney, canine kidney, feline lung, and rabbit kidney were studied. All cell cultures used were easy to manage and the last 3 are commercially-available established cell lines. The dog kidney cells were the most suitable for large-scale production of Nosema. When grown in plastic flasks with a bottom area of 75 cm2, the weekly yield from Nosema-infected canine kidney cells during the 10th to 17th week after inoculation was between 4·1 x 107 and 9·9 x 107 spores per flask. An equilibrium was obtained between the Nosema infection and the kidney cells during this time. A simple method for estimating the numbel of harvested spores is also described.

scholarly journals A Virus of the Reoviridae in Established Cell Lines of Drosophila melanogaster

1981 ◽  
Vol 54 (1) ◽  
pp. 23-31 ◽  
Author(s):  
V. E. Alatortsev ◽  
E. V. Ananiev ◽  
E. A. Gushchina ◽  
V. B. Grigoriev ◽  
B. V. Gushchin
Keyword(s):  
Drosophila Melanogaster ◽  
Cell Lines ◽  
Established Cell Lines ◽  
Established Cell
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