Probing nucleus-enriched proteins in single living cells via subcellular-resolved plasmonic immunosandwich assay

The Analyst ◽  
2021 ◽  
Author(s):  
Jia Liu ◽  
Dan Xie ◽  
Zhen Liu
Keyword(s):  
Living Cells ◽  
Nuclear Proteins ◽  
Biological Functions ◽  
Abnormal Expression ◽  
Single Living Cells ◽  
Single Living ◽  
In Cells

Nuclear proteins are crucial in cells and are greatly linked to various biological functions. Abnormal expression of nuclear proteins is associated with many diseases ranging from inflammation to cancer. However,...

scholarly journals Probing cytoplasmic and nuclear microRNAs in single living cells via plasmonic affinity sandwich assay

2018 ◽  
Vol 9 (36) ◽  
pp. 7241-7246 ◽  
Author(s):  
Jia Liu ◽  
Yanrong Wen ◽  
Hui He ◽  
Hong-Yuan Chen ◽  
Zhen Liu
Keyword(s):  
Living Cells ◽  
Sandwich Assay ◽  
Single Living Cells ◽  
Single Living ◽  
In Cells

MicroRNAs (miRNAs) regulate the expression of mRNAs in cells. The determination of subcellular miRNAs in single living cells is essential for understanding the subcellular localizations and functions of miRNAs as well as the microheterogeneity of cells.

Application of FRAP and digitized fluorescence microscopy to problems in cell membrane dynamics

1988 ◽  
Vol 46 ◽  
pp. 118-119
Author(s):  
K. Jacobson ◽  
A. Ishihara ◽  
B. Holifield ◽  
F. Zhang
Keyword(s):  
Fluorescence Microscopy ◽  
Cell Biology ◽  
Extended Period ◽  
Dynamic Structure ◽  
Living Cells ◽  
Membrane Dynamics ◽  
Molecular Cell Biology ◽  
Image Averaging ◽  
Single Living Cells ◽  
Single Living

Our laboratory is concerned with understanding the dynamic structure of the plasma membrane with particular reference to the movement of membrane constituents during cell locomotion. In addition to the standard tools of molecular cell biology, we employ both fluorescence recovery after photo- bleaching (FRAP) and digitized fluorescence microscopy (DFM) to investigate individual cells. FRAP allows the measurement of translational mobility of membrane and cytoplasmic molecules in small regions of single, living cells. DFM is really a new form of light microscopy in that the distribution of individual classes of ions, molecules, and macromolecules can be followed in single, living cells. By employing fluorescent antibodies to defined antigens or fluorescent analogs of cellular constituents as well as ultrasensitive, electronic image detectors and video image averaging to improve signal to noise, fluorescent images of living cells can be acquired over an extended period without significant fading and loss of cell viability.

scholarly journals Measurement of Separase Proteolytic Activity in Single Living Cells by a Fluorogenic Flow Cytometry Assay

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0133769 ◽  
Author(s):  
Wiltrud Haaß ◽  
Helga Kleiner ◽  
Martin C. Müller ◽  
Wolf-Karsten Hofmann ◽  
Alice Fabarius ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Proteolytic Activity ◽  
Living Cells ◽  
Flow Cytometry Assay ◽  
Single Living Cells ◽  
Single Living

Quantitative Probing of Cu2+Ions Naturally Present in Single Living Cells

2016 ◽  
Vol 28 (21) ◽  
pp. 4071-4076 ◽  
Author(s):  
Junho Lee ◽  
Hwa-Rim Lee ◽  
Jaeyeon Pyo ◽  
Youngseob Jung ◽  
Ji-Young Seo ◽  
...  
Keyword(s):  
Living Cells ◽  
Single Living Cells ◽  
Single Living

Mechanical properties of single living cells encapsulated in polyelectrolyte matrixes

2006 ◽  
Vol 124 (4) ◽  
pp. 723-731 ◽  
Author(s):  
Tiziana Svaldo Lanero ◽  
Ornella Cavalleri ◽  
Silke Krol ◽  
Ranieri Rolandi ◽  
Alessandra Gliozzi
Keyword(s):  
Mechanical Properties ◽  
Living Cells ◽  
Single Living Cells ◽  
Single Living
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