Global transcriptomic analysis of Lactobacillus delbrueckii subsp. bulgaricus ATCC11842 reveals the role of LDB_RS05285 in the post-acidification of yogurt

2021 ◽  
Author(s):  
Shuai Zhang ◽  
Dongyao Li ◽  
Xin Zhang ◽  
Yongsheng Sun ◽  
Sha Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Transcriptomic Analysis ◽  
Lactobacillus Delbrueckii ◽  
Resistant Bacteria ◽  
Lactobacillus Delbrueckii Subsp

During the storage of yogurt, acid-resistant bacteria continue to produce lactic acid (i.e., post-acidification process), leading to undesirable taste and flavor. Many methods have been proposed to inhibit post-acidification. However,...

Transposition in Lactobacillus delbrueckii subsp. bulgaricus: identification of two thermosensitive replicons and two functional insertion sequences

Microbiology ◽  
2003 ◽  
Vol 149 (6) ◽  
pp. 1503-1511 ◽  
Author(s):  
Pascale Serror ◽  
Golnar Ilami ◽  
Hichem Chouayekh ◽  
S. Dusko Ehrlich ◽  
Emmanuelle Maguin
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Bulgaricus ◽  
Insertion Sequences ◽  
Rolling Circle ◽  
Delivery Vector ◽  
Lactobacillus Delbrueckii Subsp

In this report, it is shown that the rolling circle replicon pG+host and the theta replicon pIP501 are thermosensitive in Lactobacillus delbrueckii subsp. bulgaricus (Lactobacillus bulgaricus). Using a pIP501 derivative as a delivery vector for six insertion sequences originating from lactic acid bacteria, it is shown that IS1223 and IS1201 transpose in L. bulgaricus.

Antibiotic susceptibility of different lactic acid bacteria strains

2011 ◽  
Vol 2 (4) ◽  
pp. 335-339 ◽  
Author(s):  
N. Karapetkov ◽  
R. Georgieva ◽  
N. Rumyan ◽  
E. Karaivanova
Keyword(s):  
Cell Wall ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Acidophilus ◽  
Streptococcus Thermophilus ◽  
Lactobacillus Helveticus ◽  
Lactobacillus Delbrueckii ◽  
Cell Wall Synthesis ◽  
High Susceptibility ◽  
Lactobacillus Delbrueckii Subsp

Five lactic acid bacteria (LAB) strains belonging to species Lactobacillus acidophilus, Lactobacillus helveticus, Lactobacillus delbrueckii subsp. bulgaricus, Lactobacillus delbrueckii subsp. lactis and Streptococcus thermophilus were tested for their susceptibility to 27 antibiotics. The minimum inhibitory concentrations of each antimicrobial were determined using a microdilution test. Among the strains a high susceptibility was detected for most of the cell-wall synthesis inhibitors (penicillins, cefoxitin and vancomycin) and resistance toward inhibitors of DNA synthesis (trimethoprim/sulfonamides and fluoroquinolones). Generally, the Lactobacillus strains were inhibited by antibiotics such as chloramphenicol, erythromycin and tetracycline at breakpoint levels lower or equal to the levels defined by the European Food Safety Authority. Despite the very similar profile of S. thermophilus LC201 to lactobacilli, the detection of resistance toward erythromycin necessitates the performance of additional tests in order to prove the absence of transferable resistance genes.

Production of natural folates by lactic acid bacteria starter cultures isolated from artisanal Argentinean yogurts

2012 ◽  
Vol 58 (5) ◽  
pp. 581-588 ◽  
Author(s):  
Jonathan Emiliano Laiño ◽  
Jean Guy LeBlanc ◽  
Graciela Savoy de Giori
Keyword(s):  
Lactic Acid ◽  
Folic Acid ◽  
Lactic Acid Bacteria ◽  
Culture Medium ◽  
Streptococcus Thermophilus ◽  
Starter Cultures ◽  
Lactobacillus Delbrueckii ◽  
Nonfat Milk ◽  
Native Strains ◽  
Lactobacillus Delbrueckii Subsp

Folate is a B-group vitamin that cannot be synthesized by humans and must be obtained exogenously. Although some species of lactic acid bacteria (LAB) can produce folates, little is known about the production of this vitamin by yogurt starter cultures. Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus strains were isolated from artisanal Argentinean yogurts and were grown in folate-free culture medium (FACM) and nonfat milk after which intracellular and extracellular folate production were evaluated. From the initial 92 isolated LAB strains, 4 L. delbrueckii subsp. bulgaricus and 32 S. thermophilus were able to grow in the absence of folate. Lactobacillus delbrueckii subsp. bulgaricus CRL 863 and S. thermophilus CRL 415 and CRL 803 produced the highest extracellular folate levels (from 22.3 to 135 µg/L) in FACM. In nonfat milk, these strains were able to increase the initial folate concentrations by almost 190%. This is the first report where native strains of L. delbrueckii subsp. bulgaricus were shown to produce natural folate. The LAB strains identified in this study could be used in developing novel fermented products bio-enriched in natural folates that could in turn be used as an alternative to fortification with the controversial synthetic chemical folic acid.

Continuous d-lactic acid production by a novelthermotolerant Lactobacillus delbrueckii subsp. lactis QU 41

2010 ◽  
Vol 89 (6) ◽  
pp. 1741-1750 ◽  
Author(s):  
Yukihiro Tashiro ◽  
Wataru Kaneko ◽  
Yanqi Sun ◽  
Keisuke Shibata ◽  
Kentaro Inokuma ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Delbrueckii Subsp
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