Gene knockout revealed the role of gene feoA in cell growth and division of Lactobacillus delbrueckii subsp. bulgaricus

2021 ◽  
Author(s):  
Xue Han ◽  
Yuanqiang Tu ◽  
Huiying Wu ◽  
Lijuan Zhang ◽  
Sainan Zhao
Keyword(s):  
Cell Growth ◽  
Gene Knockout ◽  
Lactobacillus Delbrueckii ◽  
Lactobacillus Delbrueckii Subsp

Global transcriptomic analysis of Lactobacillus delbrueckii subsp. bulgaricus ATCC11842 reveals the role of LDB_RS05285 in the post-acidification of yogurt

2021 ◽  
Author(s):  
Shuai Zhang ◽  
Dongyao Li ◽  
Xin Zhang ◽  
Yongsheng Sun ◽  
Sha Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Transcriptomic Analysis ◽  
Lactobacillus Delbrueckii ◽  
Resistant Bacteria ◽  
Lactobacillus Delbrueckii Subsp

During the storage of yogurt, acid-resistant bacteria continue to produce lactic acid (i.e., post-acidification process), leading to undesirable taste and flavor. Many methods have been proposed to inhibit post-acidification. However,...

scholarly journals The role of resistance to bile salts and acid tolerance of exopolysaccharides (EPSS) produced by yogurt starter bacteria

2010 ◽  
Vol 62 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Hatice Boke ◽  
Belma Aslim ◽  
Gulcin Alp
Keyword(s):  
Protective Effect ◽  
Bile Salts ◽  
Acid Tolerance ◽  
Streptococcus Thermophilus ◽  
Low Ph ◽  
Lactobacillus Delbrueckii ◽  
Viable Bacteria ◽  
Lactobacillus Delbrueckii Subsp

The aim of this study was to investigate a possible relation between EPS production and resistance to bile salts and tolerance to low pH. Eight strains which produced the highest and lowest amount of EPS (16- 211mg/l) were selected among 54 bacteria isolated from yogurt. Additionally, they were tested for resistance to bile salts (0.15, 0.3 %) and tolerance to low pH (2.0-3.0). After treatment with bile salts and acid, viable bacteria (log cfu ml-1) were determined by surface plating. The high EPS producing strains (B3, G12, W22) showed a significant (P<0.05) protective effect against low pH (pH 2.0). All Streptococcus thermophilus strains showed a higher tolerance to bile salts than the Lactobacillus delbrueckii subsp. bulgaricus strains. The high EPS-producing S. thermophilus (W22, T12) and L. bulgaricus (B3, G2) strains showed a significant (P<0.01) protective effect against bile salts (0.3 %).

Exopolysaccharides de Lactobacillus delbrueckii subsp. bulgaricus

2002 ◽  
Vol 22 (1-2) ◽  
pp. 143-149 ◽  
Author(s):  
Sandrine Petry ◽  
Satanislav Dusko Ehrlich ◽  
Emmanuelle Maguin
Keyword(s):  
Lactobacillus Delbrueckii ◽  
Lactobacillus Delbrueckii Subsp

Knockout of Ajuba Attenuates the Growth and Migration of Hepatocellular Carcinoma Cells

2020 ◽  
Vol 160 (11-12) ◽  
pp. 650-658
Author(s):  
Yichen Le ◽  
Yi He ◽  
Meirong Bai ◽  
Ying Wang ◽  
Jiaxue Wu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Growth ◽  
Cancer Progression ◽  
Normal Liver ◽  
Cell Growth And Migration ◽  
And Migration ◽  
Hcc Cells

Ajuba has been found to be mutated or aberrantly regulated in several human cancers and plays important roles in cancer progression via different signaling pathways. However, little is known about the role of Ajuba in hepatocellular carcinoma (HCC). Here, we found an upregulation of Ajuba expression in HCC tissues compared with normal liver tissues, while a poor prognosis was observed in HCC patients with high Ajuba expression. Knockout of Ajuba in HCC cells inhibited cell growth in vitro and in vivo, suppressed cell migration, and enhanced the cell apoptosis under stress. Moreover, re-expression of Ajuba in Ajuba-deficient cells could restore the phenotype of Ajuba-deficient cells. In conclusion, these results indicate that Ajuba is upregulated in HCC and promotes cell growth and migration of HCC cells, suggesting that Ajuba could possibly be a new target for HCC diagnosis and treatment.

scholarly journals Immunobiotic Feed Developed with Lactobacillus delbrueckii subsp. delbrueckii TUA4408L and the Soymilk By-Product Okara Improves Health and Growth Performance in Pigs

2021 ◽  
Vol 9 (5) ◽  
pp. 921
Author(s):  
Yoshihito Suda ◽  
Nana Sasaki ◽  
Kyoma Kagawa ◽  
Mariano Elean ◽  
Binghui Zhou ◽  
...  
Keyword(s):  
Growth Performance ◽  
Lactobacillus Delbrueckii ◽  
In Vivo Studies ◽  
Intestinal Immunity ◽  
Positive Effects ◽  
Markers Of Inflammation ◽  
Immune Health ◽  
Tlr4 Activation ◽  
Lactobacillus Delbrueckii Subsp

Lactobacillus delbrueckii subsp. delbrueckii TUA4408L is able to differentially modulate the innate immune response of porcine intestinal epithelial cells triggered by TLR4 activation. This strain also has a remarkable ability to grow on plant substrates. These two immunological and biotechnological characteristics prompted us to evaluate whether the soymilk by-product okara fermented with the TUA4408L strain can serve as an immunobiotic feed with the ability to beneficially modulate the intestinal immunity of piglets after weaning to improve their productivity. Our in vivo studies demonstrated that the administration of immunobiotic TUA4408L-fermented okara feed significantly increased piglet growth performance and meat quality. These positive effects were associated with the ability of the TUA4408L-fermented okara feed to beneficially modulate both intestinal microbiota and immunity in pigs. The immunobiotic feed improved the abundance of the beneficial bacteria Lactobacillus and Lactococcus in the gut of pigs, reduced blood markers of inflammation, and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These findings indicate that the immunobiotic TUA4408L-fermented okara feed could be an economical and environmentally friendly option to improve the growth performance and immune health of pigs.

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