Role of mono- and oligosaccharides from FOS as stabilizing agents during freeze-drying and storage of Lactobacillus delbrueckii subsp. bulgaricus

2016 ◽  
Vol 90 ◽  
pp. 251-258 ◽  
Author(s):  
Nelson Romano ◽  
Carolina Schebor ◽  
Pablo Mobili ◽  
Andrea Gómez-Zavaglia
Keyword(s):  
Freeze Drying ◽  
Lactobacillus Delbrueckii ◽  
Stabilizing Agents ◽  
And Storage ◽  
Lactobacillus Delbrueckii Subsp

Global transcriptomic analysis of Lactobacillus delbrueckii subsp. bulgaricus ATCC11842 reveals the role of LDB_RS05285 in the post-acidification of yogurt

2021 ◽  
Author(s):  
Shuai Zhang ◽  
Dongyao Li ◽  
Xin Zhang ◽  
Yongsheng Sun ◽  
Sha Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Transcriptomic Analysis ◽  
Lactobacillus Delbrueckii ◽  
Resistant Bacteria ◽  
Lactobacillus Delbrueckii Subsp

During the storage of yogurt, acid-resistant bacteria continue to produce lactic acid (i.e., post-acidification process), leading to undesirable taste and flavor. Many methods have been proposed to inhibit post-acidification. However,...

scholarly journals The role of resistance to bile salts and acid tolerance of exopolysaccharides (EPSS) produced by yogurt starter bacteria

2010 ◽  
Vol 62 (2) ◽  
pp. 323-328 ◽  
Author(s):  
Hatice Boke ◽  
Belma Aslim ◽  
Gulcin Alp
Keyword(s):  
Protective Effect ◽  
Bile Salts ◽  
Acid Tolerance ◽  
Streptococcus Thermophilus ◽  
Low Ph ◽  
Lactobacillus Delbrueckii ◽  
Viable Bacteria ◽  
Lactobacillus Delbrueckii Subsp

The aim of this study was to investigate a possible relation between EPS production and resistance to bile salts and tolerance to low pH. Eight strains which produced the highest and lowest amount of EPS (16- 211mg/l) were selected among 54 bacteria isolated from yogurt. Additionally, they were tested for resistance to bile salts (0.15, 0.3 %) and tolerance to low pH (2.0-3.0). After treatment with bile salts and acid, viable bacteria (log cfu ml-1) were determined by surface plating. The high EPS producing strains (B3, G12, W22) showed a significant (P<0.05) protective effect against low pH (pH 2.0). All Streptococcus thermophilus strains showed a higher tolerance to bile salts than the Lactobacillus delbrueckii subsp. bulgaricus strains. The high EPS-producing S. thermophilus (W22, T12) and L. bulgaricus (B3, G2) strains showed a significant (P<0.01) protective effect against bile salts (0.3 %).

scholarly journals Optimization of compatible solutes for improving survival of freeze-dried Lactobacillus delbrueckii subsp. bulgaricus using Box-Behnken design

2021 ◽  
Vol 25 (2) ◽  
pp. 301-306
Author(s):  
Guowei Shu ◽  
Bohao Li ◽  
Meng Zhang ◽  
Jie Huang ◽  
Li Chen ◽  
...  
Keyword(s):  
Survival Rate ◽  
Industrial Development ◽  
Freeze Drying ◽  
Compatible Solutes ◽  
Lactobacillus Delbrueckii ◽  
Drying Process ◽  
Sodium Glutamate ◽  
Box Behnken Design ◽  
Freeze Dried ◽  
Lactobacillus Delbrueckii Subsp

Abstract Lactobacillus delbrueckii subsp. bulgaricus is widely used in yogurt as a starter. The freeze-drying process may cause bacteria death. In the present work, the effect of three solutes (NaCl, sorbitol, and sodium glutamate) in MRS on viability of L.bulgaricus during freeze-drying was investigated. The optimal combination of adequate solutes was chosen by Box-Behnken Design. The survival rate and viable counts in freeze-dried powder, as well as the viable counts in broth, were used as responses. The results revealed that the optimum combination of solutes in MRS broth were 0.50% NaCl, 0.19% sorbitol, and 0.06% sodium glutamate. Under these optimal conditions, the survival rate was 53.2±0.14%, the viable counts in freeze-dried powder was 8.51±0.23×1010 CFU/g, and the viable counts in broth was 6.05±0.19 ×108 CFU/mL, which were increased by 17.18%, 15.94%, and 17.31%, respectively, compared to the control. This research demonstrated the possibility of viability improvement of L.bulgaricus, which may provide a feasible reference for industrial development.

Investigations on the Nature of Hemophilia A

1963 ◽  
Vol 09 (01) ◽  
pp. 030-052 ◽  
Author(s):  
Eberhard Mammen
Keyword(s):  
Factor Viii ◽  
Human Plasma ◽  
Barium Sulfate ◽  
Freeze Drying ◽  
Room Temperature ◽  
Hemophilia A ◽  
Normal Human Plasma ◽  
Normal Human ◽  
And Storage ◽  
Prothrombin Activation

SummaryIn this paper an inhibitor is described that is found in hemophilic plasma and serum different from any till now described inhibitor. The inhibitor only inhibits prothrombin activation in the “intrinsic clotting systems”. This inhibitor is probably not present in normal human plasma or serum. It is destroyed by ether and freeze drying, is labile to acid and storage at room temperature. It is stable upon dialysis and has not been adsorbed on barium sulfate, aluminum hydroxide or kaolin. It precipitates at 50% v/v saturation with alcohol. The nature of this inhibitor seems to be a protein or lipoprotein.Factor VIII was isolated from hemophilic plasma. The amount isolated was the same as from normal plasma and the activity properties were not different. Hemophiliacs have normal amounts of factor VIII.

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