scholarly journals Steroid metabolism in the cat. Biliary and urinary excretion of metabolites of [4−14C]cortisone

1969 ◽  
Vol 113 (2) ◽  
pp. 259-261 ◽  
Author(s):  
W. Taylor
Keyword(s):  
Aqueous Solution ◽  
Single Dose ◽  
Urinary Excretion ◽  
Ethyl Acetate ◽  
Single Injection ◽  
Urine Samples ◽  
Steroid Metabolism ◽  
Total Recovery ◽  
Neutral Aqueous Solution ◽  
Bile Metabolites

1. [4−14C]Cortisone was administered to anaesthetized male cats as a single injection or as a 45–60min. infusion. 2. After the single dose a total of 69·6–89·6% of the radioactivity was excreted in bile, and 0·5–7·1% in urine. After infusion total recovery in bile was 73·4–92·1%, and 1·2–1·7% in urine. 3. When bile and urine samples were hydrolysed successively by β-glucuronidase, cold acid and hot acid, most of the radioactivity was converted into substances not extractable from neutral aqueous solution by ethyl acetate–ether. 4. In bile, metabolites hydrolysable by β-glucuronidase were found in only small proportions (3–4%) of the dose.

scholarly journals Steroid metabolism in the cat. Biliary and urinary excretion of metabolites of [4-14C]testosterone

1965 ◽  
Vol 94 (3) ◽  
pp. 778-782 ◽  
Author(s):  
SEH Archer ◽  
T Scratcherd ◽  
W Taylor
Keyword(s):  
Aqueous Solution ◽  
Urinary Excretion ◽  
Single Injection ◽  
Urine Samples ◽  
Steroid Metabolism ◽  
Neutral Aqueous Solution ◽  
Consistent Change

1. [4-(14)C]Testosterone was administered intravenously to anaesthetized male cats as a single injection or as a 45-60min. infusion. 2. Most of the administered radioactivity was excreted in the bile (70-80%); only 2.9-5.5% of the dose was excreted in the urine. 3. Bile and urine samples were hydrolysed successively to yield glucuronide,;cold-acid-hydrolysed′ and; hot-acid-hydrolysed′ fractions. 4. The proportion of glucuronides in bile decreased in successive samples, but cold-and hot-acid-hydrolysed metabolites showed no consistent change. 5. After hydrolysis most of the radioactivity in both bile and urine could not be extracted by ether from neutral aqueous solution.

scholarly journals Steroid metabolism in the cat. Biliary and urinary excretion of metabolites of [4-14C]oestradiol

1970 ◽  
Vol 117 (2) ◽  
pp. 267-270 ◽  
Author(s):  
M. F. Karim ◽  
W. Taylor
Keyword(s):  
Aqueous Solution ◽  
Urinary Excretion ◽  
Ethyl Acetate ◽  
Intravenous Injection ◽  
Urine Samples ◽  
Steroid Metabolism ◽  
Single Intravenous Injection

1. [4-14C]Oestradiol was administered to seven male, seven female and two castrated male cats as a single intravenous injection. Bile and urine were collected for 6h. 2. The radioactivity was excreted mainly in the bile of all animals (53–60%); only approx. 1% of the dose appeared in the urine. 3. Bile and urine samples were hydrolysed successively by β-glucuronidase, cold acid and hot acid. There were significant differences (P<0.005) between the percentage of the dose present in the bile fractions hydrolysed by β-glucuronidase (male, 9.0±1.7%; female, 18.6±1.45%) and by cold acid (male, 18.9±1.44%; female 12.1±1.02%). The excretion of radioactivity in these fractions by the castrated male cats was closer to that of female cats. 4. Approx. 20–27% of the dose could not be extracted from aqueous solution (pH10.5) by ethyl acetate–ether after hydrolysis.

scholarly journals Steroid metabolism in the rabbit. Biliary and urinary excretion of metabolites of [4-14C]progesterone

1965 ◽  
Vol 97 (1) ◽  
pp. 89-94 ◽  
Author(s):  
W Taylor ◽  
T Scratcherd
Keyword(s):  
Single Dose ◽  
New Zealand ◽  
Urinary Excretion ◽  
Alkaline Solution ◽  
Single Injection ◽  
Steroid Metabolism ◽  
Apparent Difference ◽  
Total Recovery ◽  
Male And Female ◽  
New Zealand White Rabbits

1. [4-(14)C]Progesterone was administered intravenously to anaesthetized male and female New Zealand White rabbits as a single injection or as a 45-60min. infusion. 2. After a single dose about 60% of the radioactivity was recovered in 6hr., and twice as much radioactivity was present in bile as in urine. After infusion total recovery of radioactivity was only about 40% in 6hr., but the relative proportions of metabolites in bile and urine were about the same as after a single dose. 3. Bile and urine samples were hydrolysed successively by beta-glucuronidase, cold acid and hot acid. 4. In bile the major proportion of metabolites appeared in the glucuronide fraction; in urine beta-glucuronidase hydrolysis yielded the greatest amounts of ether-extractable radioactivity, but the greatest proportion of radioactivity could not be extracted by ether from an alkaline solution of the hydrolysed urine. 5. There was no apparent difference in the quantity or distribution of metabolites excreted by male and female animals.

scholarly journals Steroid metabolism in the rabbit. Biliary and urinary excretion of metabolites of [4-14C]cortisone

1970 ◽  
Vol 117 (2) ◽  
pp. 263-265 ◽  
Author(s):  
W. Taylor
Keyword(s):  
Aqueous Solution ◽  
New Zealand ◽  
Urinary Excretion ◽  
Single Injection ◽  
Steroid Metabolism ◽  
Equal Proportion ◽  
Male And Female ◽  
New Zealand White Rabbits ◽  
The Mean ◽  
Total Excretion

1. [4-14C]Cortisone was administered to anaesthetized male and female New Zealand White rabbits as a single injection or as a 45–60min infusion. 2. The method of administration of the steroid did not significantly affect the total excretion of radioactivity in bile and urine [83.8±10.8%(s.d.)]. 3. The mean ratio of metabolites in urine to those in bile was 0.97±0.23% (range 0.64–1.3). 4. When bile and urine samples were hydrolysed successively by β-glucuronidase, cold acid and hot acid, neutral metabolites extracted by ethyl acetate–ether were found mainly after hydrolysis by β-glucuronidase. 5. An approximately equal proportion of the dose was converted into substances not extractable from alkaline aqueous solution after hydrolysis.

EFFECTS OF Zn-DTPA ON ESSENTIAL TRACE METALS IN RATS

1994 ◽  
Vol 04 (04) ◽  
pp. 269-273 ◽  
Author(s):  
I. SATO ◽  
N. MATSUSAKA ◽  
H. KOBAYASHI ◽  
K. SERA ◽  
S. FUTATSUGAWA ◽  
...  
Keyword(s):  
Single Dose ◽  
Trace Metals ◽  
Single Injection ◽  
Chelating Agent ◽  
Urine Samples ◽  
Urinary Concentration ◽  
Essential Metals ◽  
Tissue Samples ◽  
Daily Dose ◽  
Almost All

Zn-DTPA was injected intraperitoneally into rats (30 µmol/kg) with a single dose or a daily dose for 10 consecutive days. Urine samples were collected for 24 hours after the single injection and tissue samples were excised 20 hours after the final of the consecutive injections. Elements in these samples were analyzed by PIXE. Zn-DTPA raised the urinary concentration of zinc and copper, and their urinary losses caused by Zn-DTPA were estimated to be 1.9 and 0.01 mg/30μmol Zn-DTPA, respectively. The concentration of metals in the liver, kidneys, brain and femurs remained unchanged. These results suggest that almost all of Zn-DTPA is excreted into the urine without replacement with other metals, and so this chelating agent may not induce any deficiencies of essential metals when animals are treated with a dose of 30 µmol/kg per day.

STUDIES ON THE MECHANISM OF DECIDUALIZATION. I.

1963 ◽  
Vol 42 (2) ◽  
pp. 225-232 ◽  
Author(s):  
M. C. Shelesnyak ◽  
Peretz F. Kraicer ◽  
Gerard H. Zeilmaker
Keyword(s):  
Single Dose ◽  
Single Injection

ABSTRACT The concept of an oestrogen surge as a prerequisite for successful decidualization of the progravid uterus and consequently nidation in the rat was tested. Experiments were designed to see whether administration of an oestrogen antagonist at specific times would block decidualization (and nidation); whether the ovary was the source of the oestrogen, and whether a single dose of oestrogen, in the absence of the ovary (if the ovary is the source) would allow the uterus to respond to a decidualizing stimulus. It was found that when given prior to the surge, the oestrogen antagonist prevented decidualization and interfered with nidation. The ovary is thus the source of the oestrogen; and a single injection of oestradiol can act as a substitute for the surge.

Liver preneoplastic changes in mice treated with the herbicide fomesafen

1999 ◽  
Vol 18 (5) ◽  
pp. 338-344 ◽  
Author(s):  
J Krijt ◽  
P Stránská ◽  
J Sanitraák ◽  
A Chlumská ◽  
F Fakan
Keyword(s):  
Single Dose ◽  
Single Injection ◽  
Diphenyl Ether ◽  
Liver Histology ◽  
Mouse Strains ◽  
Toxicological Evaluation ◽  
Liver Nodules ◽  
Icr Mice ◽  
Mice Liver ◽  
Foci Of Altered Hepatocytes

1 Effect of the diphenyl ether herbicide fomesafen on liver preneoplastic changes and porphyrin biosynthesis was examined in male C57BL/6J mice (0.23% in the diet for 14 months) and ICR mice (0.3% in the diet for 50 weeks). Fomesafen treatment resulted in preneoplastic changes (liver nodules and foci of altered hepatocytes) in both strains, uroporphyria developed only in ICR mice. 2 Iron pretreatment (600 mg/kg as a single dose) accelerated the development of fomesafen-induced preneoplastic changes in both mouse strains. The number of foci containing altered hepatocytes, as well as the number and size of liver nodules, were increased in iron-pretreated animals. 3 A single injection of iron induced marked uroporphyria in C57BL/6J mice after 14 months (liver porphyrin content 102 nmol/g). This uroporphyria was further potentiated by fomesafen administration (208 nmol/g). 4 In ICR mice, liver histology was apparently normal after a 3 month recovery from fomesafen treatment (0.32% for 9 months). Liver porphyrin content (260 nmol/g) started to decrease immediately after fomesafen withdrawal, but was still significantly elevated after 3 months (5 nmol/g), as compared to controls (1 nmol/g). 5 It is concluded that the toxicological evaluation of fomesafen should focus on liver porphyrin biosynthesis.

Sign in / Sign up

Export Citation Format

Share Document