Isolation and characterization of a myeloma–spleen-cell hybrid producing antibody to phenylalanine hydroxylase
Keyword(s):
Application of the technique of myeloma–spleen-cell fusion [Kohler & Milstein (1975) Nature (London) 256, 495–497] has allowed the isolation of a cell colony that produced a monoclonal antibody against monkey liver phenylalanine hydroxylase. The antibody exhibited cross-reactivity against hepatic phenylalanine hydroxylase from other mammalian species, including human, rat and mouse. Cross-reactivity was established by (a) enzyme-inhibition assay, (b) double-immunodiffusion reaction, and (c) two-dimensional polyacrylamide-gel-electrophoretic analysis of immunoprecipitate. The various properties of the monoclonal antibody and its use in the study of mammalian phenylalanine hydroxylase are presented.
1985 ◽
Vol 98
(1)
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pp. 209-217
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2001 ◽
Vol 56
(3-4)
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pp. 402-405
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2002 ◽
Vol 68
(9)
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pp. 4390-4398
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1980 ◽
Vol 87
(3)
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pp. 755-763
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2005 ◽
Vol 71
(8)
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pp. 4487-4496
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2012 ◽
Vol 95
(2)
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pp. 372-376
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1986 ◽
Vol 46
(2)
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pp. 435-439
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2008 ◽
Vol 76
(4)
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pp. 326-336
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