scholarly journals Identification and characterization of the gene encoding the human phosphopantetheine adenylyltransferase and dephospho-CoA kinase bifunctional enzyme (CoA synthase)

2002 ◽  
Vol 365 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Suren AGHAJANIAN ◽  
D.Margaret WORRALL
Keyword(s):  
Biosynthetic Pathway ◽  
Sequence Similarity ◽  
Kinetic Properties ◽  
Sequence Information ◽  
Bifunctional Enzyme ◽  
Gene Encoding ◽  
Pig Liver ◽  
Identification And Characterization ◽  
Similar Kinetic

The final two enzymes in the CoA biosynthetic pathway, phosphopantetheine adenylyltransferase (PPAT; EC 2.7.7.3) and dephospho-CoA kinase (DPCK; EC 2.7.1.24), are separate proteins in prokaryotes, but exist as a bifunctional enzyme in pig liver. In the present study we have obtained sequence information from purified pig-liver enzyme, and identified the corresponding cDNA in a number of species. The human gene localizes to chromosome 17q12-21 and contains regions with sequence similarity to the monofunctional Escherichia coli DPCK and PPAT. The recombinant 564-amino-acid human protein confirmed the associated transferase and kinase activities, and gave similar kinetic properties to the wild-type pig enzyme.

scholarly journals Identification and characterization of YLR328W, theSaccharomyces cerevisiaestructural gene encoding NMN adenylyltransferase. Expression and characterization of the recombinant enzyme

FEBS Letters ◽  
1999 ◽  
Vol 455 (1-2) ◽  
pp. 13-17 ◽  
Author(s):  
Monica Emanuelli ◽  
Francesco Carnevali ◽  
Maria Lorenzi ◽  
Nadia Raffaelli ◽  
Adolfo Amici ◽  
...  
Keyword(s):  
Recombinant Enzyme ◽  
Gene Encoding ◽  
Identification And Characterization

Biosynthesis of the uridine-derived nucleoside antibiotic A-94964: identification and characterization of the biosynthetic gene cluster provide insight into the biosynthetic pathway

2019 ◽  
Vol 17 (3) ◽  
pp. 461-466 ◽  
Author(s):  
Taro Shiraishi ◽  
Makoto Nishiyama ◽  
Tomohisa Kuzuyama
Keyword(s):  
Gene Cluster ◽  
In Silico ◽  
Biosynthetic Pathway ◽  
Gene Deletion ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Identification And Characterization ◽  
Insight Into

The biosynthetic pathway of the uridine-derived nucleoside antibiotic A-94964 was proposed via in silico analysis coupled with gene deletion experiments.

scholarly journals Characterization of Flagellotropic, Chi-Like Salmonella Phages Isolated from Thai Poultry Farms

Viruses ◽  
2019 ◽  
Vol 11 (6) ◽  
pp. 520 ◽  
Author(s):  
Preeda Phothaworn ◽  
Matthew Dunne ◽  
Rattaya Supokaivanich ◽  
Catherine Ong ◽  
Jiali Lim ◽  
...  
Keyword(s):  
Salmonella Enterica Serovar Typhimurium ◽  
Sequence Similarity ◽  
Phage Type ◽  
Pcr Amplification ◽  
Phylogenomic Analysis ◽  
Monophyletic Clade ◽  
Gene Encoding ◽  
Poultry Farms ◽  
Serovar Typhimurium

Despite a wealth of knowledge on Salmonella phages worldwide, little is known about poultry-associated Salmonella phages from Thailand. Here, we isolated 108 phages from Thai poultry farms that infect Salmonella enterica serovar Typhimurium. Phages STm101 and STm118 were identified as temperate Siphoviridae phages. Genome sequencing and analyses revealed these phages share approximately 96% nucleotide sequence similarity to phage SPN19, a member of the Chi-like virus genus. PCR amplification of the gene encoding capsid protein E of the Chi-like phage was positive for 50% of phage isolates, suggesting a predominance of this phage type among the sampled poultry farms. In addition to the flagella, two phages required the lipopolysaccharide to infect and lyse Salmonella. Furthermore, phylogenomic analysis demonstrated that phages STm101 and STm118 formed a monophyletic clade with phages isolated from Western countries, but not from closer isolated phages from Korea. However, further investigation and more phage isolates are required to investigate possible causes for this geographic distribution.

scholarly journals Identification and Characterization of a Porcine Torovirus

1998 ◽  
Vol 72 (5) ◽  
pp. 3507-3511 ◽  
Author(s):  
A. Kroneman ◽  
L. A. H. M. Cornelissen ◽  
M. C. Horzinek ◽  
R. J. de Groot ◽  
H. F. Egberink
Keyword(s):  
Longitudinal Study ◽  
Neutralizing Antibodies ◽  
Rt Pcr ◽  
Gene Encoding ◽  
Nucleocapsid Gene ◽  
Sequence Identity ◽  
Young Pigs ◽  
Porcine Torovirus ◽  
Identification And Characterization

ABSTRACT A porcine torovirus (PoTV) was identified and characterized; it is a novel member of the genus Torovirus (familyCoronaviridae, order Nidovirales), closely related to but clearly distinct from the already recognized equine torovirus (ETV) and bovine torovirus (BoTV) representatives. Immunoelectron microscopy of feces from piglets revealed elongated, 120- by 55-nm particles which were recognized by a torovirus-specific antiserum. Amplification by reverse transcriptase (RT) PCR with primers designed to detect conserved regions (on the basis of the genomes of BoTV strain Breda and ETV strain Berne) resulted in the identification of the 489-bp nucleocapsid gene, encoding a 18.7-kDa protein. The sequence identity in this region between PoTV and both ETV and BoTV was only about 68%, whereas the latter two show 81% identity. Neutralizing antibodies directed against ETV were found in sera of adult and young pigs. In all 10 herds sampled, seropositive animals were present, and 81% of randomly selected adult sows possessed antibodies. A longitudinal study with RT PCR showed that piglets shed virus in the feces for 1 or more days, starting 4 to 14 days after weaning.

scholarly journals Identification and Characterization of the Murine and Human Gene Encoding the Tuberoinfundibular Peptide of 39 Residues

Endocrinology ◽  
2002 ◽  
Vol 143 (3) ◽  
pp. 1047-1057 ◽  
Author(s):  
Markus R. John ◽  
Maya Arai ◽  
David A. Rubin ◽  
Kenneth B. Jonsson ◽  
Harald Jüppner
Keyword(s):  
Human Gene ◽  
Gene Encoding ◽  
Tuberoinfundibular Peptide ◽  
Identification And Characterization
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