scholarly journals Towards abolition of immunogenic structures in insect cells: characterization of a honey-bee (Apis mellifera) multi-gene family reveals both an allergy-related core α1,3-fucosyltransferase and the first insect Lewis-histo-blood-group-related antigen-synthesizing enzyme

2007 ◽  
Vol 402 (1) ◽  
pp. 105-115 ◽  
Author(s):  
Dubravko Rendić ◽  
Jaroslav Klaudiny ◽  
Ute Stemmer ◽  
Julia Schmidt ◽  
Katharina Paschinger ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Bee Venom ◽  
Cloning And Expression ◽  
Biochemical Assays ◽  
Venom Glands ◽  
Insect Cell Lines ◽  
The Brain

Glycoproteins from honey-bee (Apis mellifera), such as phospholipase A2 and hyaluronidase, are well-known major bee-venom allergens. They carry N-linked oligosaccharide structures with two types of α1,3-fucosylation: the modification by α1,3-fucose of the innermost core GlcNAc, which constitutes an epitope recognized by IgE from some bee-venom-allergic patients, and an antennal Lewis-like GalNAcβ1,4(Fucα1,3)GlcNAc moiety. We now report the cloning and expression of two cDNAs encoding the relevant active α1,3-FucTs (α1,3-fucosyltransferases). The first sequence, closest to that of fruitfly (Drosophila melanogaster) FucTA, was found to be a core α1,3-FucT (EC 2.4.1.214), as judged by several enzyme and biochemical assays. The second cDNA encoded an enzyme, most related to Drosophila FucTC, that was shown to be capable of generating the Lex [Galβ1-4(Fucα1-3)GlcNAc] epitope in vitro and is the first Lewis-type α1,3-FucT (EC 2.4.1.152) to be described in insects. The transcription levels of these two genes in various tissues were examined: FucTA was found to be predominantly expressed in the brain tissue and venom glands, whereas FucTC transcripts were detected at highest levels in venom and hypopharyngeal glands. Very low expression of a third homologue of unknown function, FucTB, was also observed in various tissues. The characterization of these honey-bee gene products not only accounts for the observed α1,3-fucosylation of bee-venom glycoproteins, but is expected to aid the identification and subsequent down-regulation of the FucTs in insect cell lines of biotechnological importance.

scholarly journals Synthesis, Quantification, and Characterization of Fatty Acid Amides from In Vitro and In Vivo Sources

Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2543
Author(s):  
Ruidong Ni ◽  
Suzeeta Bhandari ◽  
Perry R. Mitchell ◽  
Gabriela Suarez ◽  
Neel B. Patel ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Fatty Acid ◽  
Apis Mellifera ◽  
Chemical Synthesis ◽  
Acid Amide ◽  
Fatty Acid Amides ◽  
Fatty Acid Amide

Fatty acid amides are a diverse family of underappreciated, biologically occurring lipids. Herein, the methods for the chemical synthesis and subsequent characterization of specific members of the fatty acid amide family are described. The synthetically prepared fatty acid amides and those obtained commercially are used as standards for the characterization and quantification of the fatty acid amides produced by biological systems, a fatty acid amidome. The fatty acid amidomes from mouse N18TG2 cells, sheep choroid plexus cells, Drosophila melanogaster, Bombyx mori, Apis mellifera, and Tribolium castaneum are presented.

scholarly journals 07-P009 Doublesex variants in the honey bee, Apis mellifera: Lack of sex-specific expression and characterization of novel transcripts

2009 ◽  
Vol 126 ◽  
pp. S139 ◽  
Author(s):  
Flavia C. Freitas ◽  
Alexandre S. Cristino ◽  
Francis M. Nunes ◽  
Zila L. Simoes
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Specific Expression ◽  
Novel Transcripts

High-resolution (mtDNA) melting analysis for simple and efficient characterization of Africanized honey bee Apis mellifera (Hymenoptera:Apidae)

Genetica ◽  
2021 ◽  
Author(s):  
Leonardo P. Porrini ◽  
Constanza Brasesco ◽  
Matias Maggi ◽  
Martín J. Eguaras ◽  
Silvina Quintana
Keyword(s):  
Apis Mellifera ◽  
High Resolution ◽  
Honey Bee ◽  
Africanized Honey Bee ◽  
Melting Analysis

scholarly journals Toxicity Evaluation of Selected Plant Water Extracts on a Honey Bee (Apis mellifera L.) Larvae Model

Animals ◽  
2022 ◽  
Vol 12 (2) ◽  
pp. 178
Author(s):  
Roksana Kruszakin ◽  
Paweł Migdal
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Larval Rearing ◽  
Distilled Water ◽  
Chelidonium Majus ◽  
Freeze Dried ◽  
Study Laboratory ◽  
The Impact

So far, larval rearing in vitro has been an important method in the assessment of bee toxicology, particularly in pesticide risk assessment. However, natural products are increasingly used to control honey bee pathogens or to enhance bee immunity, but their effects on honey bee larvae are mostly unknown. In this study, laboratory studies were conducted to determine the effects of including selected aqueous plant infusions in the diet of honey bee (Apis mellifera L.) larvae in vitro. The toxicity of infusions from three different plant species considered to be medicinal plants was evaluated: tansy (Tanacetum vulgare L.), greater celandine (Chelidonium majus L.), and coriander (Coriandrum sativum L.). The impact of each on the survival of the larvae of honey bees was also evaluated. One-day-old larvae were fed a basal diet consisting of distilled water, sugars (glucose and fructose), yeast extract, and freeze-dried royal jelly or test diets in which distilled water was replaced by plant infusions. The proportion of the diet components was adjusted to the age of the larvae. The larvae were fed twice a day. The experiment lasted seven days. Significant statistical differences in survival rates were found between groups of larvae (exposed or not to the infusions of tansy, greater celandine, and coriander). A significant decrease (p < 0.05) in the survival rate was observed in the group with the addition of a coriander herb infusion compared to the control. These results indicate that plant extracts intended to be used in beekeeping should be tested on all development stages of honey bees.

scholarly journals Morphological and functional characterization of honey bee, Apis mellifera, hemocyte cell communities

Apidologie ◽  
2018 ◽  
Vol 49 (3) ◽  
pp. 397-410 ◽  
Author(s):  
Rodney T. Richardson ◽  
Megan N. Ballinger ◽  
Feng Qian ◽  
John W. Christman ◽  
Reed M. Johnson
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Functional Characterization

The effect of Bt Cry9Ee toxin on honey bee brood and adults reared in vitro, Apis mellifera (Hymenoptera: Apidae)

2019 ◽  
Vol 181 ◽  
pp. 381-387 ◽  
Author(s):  
Pingli Dai ◽  
Mengyue Wang ◽  
Lili Geng ◽  
Zhenxiong Yan ◽  
Yang Yang ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee
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