Accumulation of free complex-type N-glycans in MKN7 and MKN45 stomach cancer cells

2008 ◽  
Vol 413 (2) ◽  
pp. 227-237 ◽  
Author(s):  
Aya Ishizuka ◽  
Yuki Hashimto ◽  
Ryosuke Naka ◽  
Mitsuhiro Kinoshita ◽  
Kazuaki Kakehi ◽  
...  
Keyword(s):  
Stomach Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Mammalian Cells ◽  
Comprehensive Analysis ◽  
Complex Type ◽  
Free Oligosaccharides ◽  
Biochemical Analyses ◽  
Glycosylation Reaction ◽  
Cytosolic Sialidase

During the N-glycosylation reaction, it has been shown that ‘free’ N-glycans are generated either from lipid-linked oligosaccharides or from misfolded glycoproteins. In both cases, occurrence of high mannose-type free glycans is well-documented, and the molecular mechanism for their catabolism in the cytosol has been studied. On the other hand, little, if anything, is known with regard to the accumulation of more processed, complex-type free oligosaccharides in the cytosol of mammalian cells. During the course of comprehensive analysis of N-glycans in cancer cell membrane fractions [Naka et al. (2006) J. Proteome Res. 5, 88–97], we found that a significant amount of unusual, complex-type free N-glycans were accumulated in the stomach cancer-derived cell lines, MKN7 and MKN45. The most abundant and characteristic glycan found in these cells was determined to be NeuAcα2-6Galβ1-4GlcNAcβ1-2Manα1-3Manβ1-4GlcNAc. Biochemical analyses indicated that those glycans found were cytosolic glycans derived from lysosomes due to low integrity of the lysosomal membrane. Since the accumulation of these free N-glycans was specific to only two cell lines among the various cancer cell lines examined, these cytosolic N-glycans may serve as a specific biomarker for diagnosis of specific tumours. A cytosolic sialidase, Neu2, was shown to be involved in the degradation of these sialoglycans, indicating that the cytosol of mammalian cells might be equipped for metabolism of complex-type glycans.

scholarly journals Comprehensive analysis of the gene expression profiles in human gastric cancer cell lines

Oncogene ◽  
2002 ◽  
Vol 21 (42) ◽  
pp. 6549-6556 ◽  
Author(s):  
Jiafu Ji ◽  
Xin Chen ◽  
Suet Yi Leung ◽  
Jen-Tsan A Chi ◽  
Kent Man Chu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Gastric Cancer Cell ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Comprehensive Analysis ◽  
Human Gastric Cancer ◽  
Gastric Cancer Cell Lines

scholarly journals The mechanism of c-erbB-2 gene product increase in stomach cancer cell lines

1993 ◽  
Vol 8 (2) ◽  
pp. 153 ◽  
Author(s):  
Chang Dae Bae ◽  
Seong Eun Park ◽  
Yeon Sun Seong ◽  
Seung Won Kim ◽  
Joo Bae park ◽  
...  
Keyword(s):  
Gene Product ◽  
Stomach Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Product Increase

The effects of HOXB7 in the promotion of migration, invasion and anti-apoptosis in gastric cancer.

2016 ◽  
Vol 34 (4_suppl) ◽  
pp. 46-46
Author(s):  
Moon Kyung Joo ◽  
Jong-Jae Park ◽  
Hyo Soon Yoo ◽  
Beom Jae Lee ◽  
Hoon Jai Chun ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Cancer ◽  
Intestinal Metaplasia ◽  
Stomach Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Chronic Gastritis ◽  
Cancer Cell Lines ◽  
Akt Pathway ◽  
Apoptotic Effect

46 Background: HOX genes, a subset of the homeobox gene family, are known to be aberrantly expressed in various types of cancers. Among them, HOXB7 recently has been reported to be highly expressed in esophageal or colorectal cancers. We aimed in this study to demonstrate the critical roles of HOXB7 in development and progression of gastric cancer. Methods: We screened gene and protein expression of HOXB7 in various gastric cancer cell lines, and also compared gene expression level of HOXB7 among chronic gastritis, intestinal metaplasia and gastric cancer tissues. To figure out the oncogenic effects of HOXB7 in vitro, we performed annexin-V assay, wound closure assay and Matrigel invasion assay. We performed Western blot analysis to examine the impact of HOXB7 on AKT pathway. Results: Both mRNA and protein was substantially expressed in stomach cancer cell lines (SNU-638, SNU-719, MKN-28, MKN-45, AGS, KATO-III, NCI-N87), however, they were nearly abolished in normal gastric tissues. Gene expression was significantly higher in primary or metastatic stomach cancer, compared with chronic gastritis or intestinal metaplasia. Knockdown of HOXB7 by transfection with siRNA in AGS and SNU-638 cells significantly inhibited migration and invasion, and showed anti-apoptotic effect. Because a previous study demonstrated that enforced expression of HOXB7 could enhance PI3K/AKT pathway activity in colon cancer cells (Liao W.T. et al, Clin Cancer Res; 17(11) June 1, 2011), we investigated the modulation of AKT/PTEN pathway by HOXB7 and observed that knockdown of HOXB7 significantly downregulated phospho-AKT and upregulated PTEN in both cell lines. Furthermore, target gene products of AKT pathway including cyclin D1, survivin, Bcl-xL and MMP-9 were significantly downregulated by siHOXB7. Conclusions: Our findings suggest that HOXB7 might play a crucial role in migration, invasion and anti-apoptotic effect via modulating AKT/PTEN pathway.

scholarly journals Mechanistic Aspects of Green Tea as a Cancer Preventive: Effect of Components on Human Stomach Cancer Cell Lines

1999 ◽  
Vol 90 (7) ◽  
pp. 733-739 ◽  
Author(s):  
Sachiko Okabe ◽  
Yumiko Ochiai ◽  
Miwa Aida ◽  
Keunchil Park ◽  
Seong-Jin Kim ◽  
...  
Keyword(s):  
Stomach Cancer ◽  
Cell Lines ◽  
Green Tea ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Human Stomach ◽  
Preventive Effect ◽  
Human Stomach Cancer

scholarly journals Cytotoxic Phenanthrene, Dihydrophenanthrene, and Dihydrostilbene Derivatives and Other Aromatic Compounds from Combretum laxum

Molecules ◽  
2020 ◽  
Vol 25 (14) ◽  
pp. 3154
Author(s):  
Eder Bisoli ◽  
Talita Vilalva Freire ◽  
Nídia Cristiane Yoshida ◽  
Walmir Silva Garcez ◽  
Lyara Meira Marinho Queiróz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Mammalian Cells ◽  
Human Cancer ◽  
Radical Scavenging ◽  
Cancer Cell Lines ◽  
In Vitro Cytotoxicity ◽  
Mass Spectrometry Data ◽  
Spectroscopic Techniques

The chemical investigation of the roots and stems of Combretum laxum yielded a new dihydrostilbene derivative, 4′-hydroxy-3,3′,4-trimethoxy-5-(3,4,5-trimethoxyphenoxy)-bibenzyl (1), two phenanthrenes (2–3), and three dihydrophenanthrenes (4–6), along with one lignan, three triterpenoids, one aurone, one flavone, one naphthoquinone, and two benzoic acid derivatives. Their structures were determined by 1D and 2D nuclear magnetic resonance (NMR) spectroscopic techniques and/or mass spectrometry data. The occurrence of dihydrostilbenoid, phenanthrene and dihydrophenanthrene derivatives is unprecedented in a Combretum species native to the American continent. 2,7-Dihydroxy-4,6-dimethoxyphenanthrene, 2,6-dihydroxy-4,7-dimethoxy-9,10-dihydrophenanthrene and 5-O-methyl apigenin are novel findings in the Combretaceae, as is the isolation of compounds belonging to the chemical classes of aurones and naphthoquinones, while (+)-syringaresinol is reported for the first time in the genus Combretum. Compounds 1–6 were also evaluated for their in vitro cytotoxicity against five human cancer cell lines, and radical-scavenging ability against 1,1-diphenyl-2-picryl-hydrazyl (DPPH). 6-Methoxycoelonin (4) was the most cytotoxic against melanoma cells (IC50 2.59 ± 0.11 µM), with a high selectivity index compared with its toxicity against nontumor mammalian cells (SI 25.1). Callosin (6), despite exhibiting the strongest DPPH-scavenging activity (IC50 17.7 ± 0.3 µM), proved marginally inhibitory to the five cancer cell lines tested, indicating that, at least for these cells, antioxidant potential is unrelated to antiproliferative activity.

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