Trace analysis of methylated and hydroxymethylated cytosines in DNA by isotope-dilution LC–MS/MS: first evidence of DNA methylation in Caenorhabditis elegans

2014 ◽  
Vol 465 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Chiung-Wen Hu ◽  
Jian-Lian Chen ◽  
Yu-Wen Hsu ◽  
Cheng-Chieh Yen ◽  
Mu-Rong Chao

We developed an online solid-phase extraction LC–MS/MS method to simultaneously measure 5-methyl-2′-deoxycytidine and 5-hydroxymethyl-2′-deoxycytidine in DNA. We demonstrated that 5-methyl-2′-deoxycytidine is present in Caenorhabditis elegans and its level was regulated by decitabine or cadmium in a dose–response manner.

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