scholarly journals Acetylation of spermidine and methylglyoxal bis(guanylhydrazone) in baby-hamster kidney cells (BHK-21/C13)

1988 ◽  
Vol 253 (1) ◽  
pp. 223-227 ◽  
Author(s):  
H M Wallace ◽  
M E Nuttall ◽  
F C Robinson
Keyword(s):  
Enzyme Activity ◽  
Proteolytic Degradation ◽  
Nuclear Fraction ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Acetyl Coa ◽  
Baby Hamster Kidney Cells

Treatment of BHK-21/C13 cells with methylglyoxal bis(guanylhydrazone) (MGBG) induced the cytosolic form of spermidine N1-acetyltransferase. It stabilized the enzyme against proteolytic degradation, but the drug did not affect the enzyme activity in vitro. MGBG was itself acetylated by BHK-21/C13 cells, but at only one-tenth the rate at which spermidine was acetylated. Acetylation occurred almost exclusively in the nuclear fraction. The product was identified as N-acetyl-MGBG by h.p.l.c., by using [3H]acetyl-CoA and [14C]MGBG as co-substrates. The results suggest that the acetylation of MGBG by BHK-21/C13 cells occurs by a different acetyltransferase enzyme from that which acetylates spermidine.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

scholarly journals Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica.

1997 ◽  
Vol 41 (10) ◽  
pp. 2286-2288 ◽  
Author(s):  
S Ankri ◽  
T Miron ◽  
A Rabinkov ◽  
M Wilchek ◽  
D Mirelman
Keyword(s):  
Alcohol Dehydrogenase ◽  
Entamoeba Histolytica ◽  
Kidney Cells ◽  
Cysteine Proteinases ◽  
Baby Hamster Kidney ◽  
Important Contributor ◽  
Cytopathic Effects ◽  
Baby Hamster Kidney Cells

The ability of Entamoeba histolytica trophozoites to destroy monolayers of baby hamster kidney cells is inhibited by allicin, one of the active principles of garlic. Cysteine proteinases, an important contributor to amebic virulence, as well as alcohol dehydrogenase, are strongly inhibited by allicin.

scholarly journals Cytotoxicity of Pristine C<sub>60</sub> Fullerene on Baby Hamster Kidney Cells in Solution

2012 ◽  
Vol 03 (03) ◽  
pp. 385-390 ◽  
Author(s):  
Shufang Liu ◽  
Haijie Liu ◽  
Zhijuan Yin ◽  
Kai Guo ◽  
Xibao Gao
Keyword(s):  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Baby Hamster Kidney Cells

scholarly journals Sequences in pol Are Required for Transfer of Human Foamy Virus-Based Vectors

1998 ◽  
Vol 72 (7) ◽  
pp. 5510-5516 ◽  
Author(s):  
Otto Erlwein ◽  
Paul D. Bieniasz ◽  
Myra O. McClure
Keyword(s):  
Cell Lines ◽  
Foamy Virus ◽  
Infectious Clone ◽  
Helper Virus ◽  
Kidney Cells ◽  
Human Foamy Virus ◽  
Vector Systems ◽  
Sequence Requirements ◽  
Baby Hamster Kidney Cells

ABSTRACT A series of vectors with heterologous genes was constructed from HSRV1, an infectious clone of human foamy virus (HFV), and transfected into baby hamster kidney cells to generate stably transfected vector cell lines. Two cis-acting sequences were required to achieve efficient rescue by helper virus. The first element was located at the 5′ end upstream of position 1274 of the proviral DNA. Interestingly, a mutation in the leader sequence which decreased the ability to dimerize in vitro inhibited transfer by helper HFV. A second element that was important for vector transfer was located in thepol gene between positions 5638 and 6317. Constructs lacking this element were only poorly transferred by helper HFV, even though their RNA was produced in the vector cell lines. This finding rules out the possibility that the observed lack of transfer was due to RNA instability. A minimal vector containing only these two elements could be successfully delivered by helper HFV, confirming that all essential cis-acting sequences were present. The presence of a sequence described as a second polypurine tract in HFV was not necessary for transfer. Our data identified the minimal sequence requirements for HFV vector transfer for the development of useful vector systems.

scholarly journals Uptake and utilization of 5'-methylthioadenosine by cultured baby-hamster kidney cells

1982 ◽  
Vol 204 (3) ◽  
pp. 803-807 ◽  
Author(s):  
T O Eloranta ◽  
K Tuomi ◽  
A M Raina
Keyword(s):  
Nucleic Acids ◽  
Cellular Metabolism ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Purine Nucleotide ◽  
Salvage Pathway ◽  
Baby Hamster Kidney Cells

5'-Methylthioadenosine was taken up and immediately metabolized further by cultured baby-hamster kidney cells during the exponential phase of growth. The adenine moiety supplied the purine-nucleotide pool via the salvage pathway and was efficiently incorporated into nucleic acids. Catabolites of methylthioadenosine excreted by the cells included adenine, purinic compounds and metabolites of the ribose portion. 5'-Methylthiotubercidin had no significant effect on the cellular metabolism of methyl-thioadenosine, but greatly inhibited its uptake. erythro-9-(2-Hydroxy-3-nonyl)adenine had no effect on the uptake, but markedly interfered with the further utilization of methylthioadenosine after cleavage in the cells.

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