scholarly journals Identification of Rhizobium leguminosarum and Rhizobium sp. (Cicer) strains using a custom Fatty Acid Methyl Ester (FAME) profile library

1999 ◽  
Vol 86 (1) ◽  
pp. 78-86 ◽  
Author(s):  
K. E. Dunfield ◽  
L. J. C. Xavier ◽  
J. J. Germida
2021 ◽  
Author(s):  
Randy F Lacey ◽  
Blake A Sullivan-Hill ◽  
Julie R Deslippe ◽  
Robert A Keyzers ◽  
Monica L Gerth

Phytophthora diseases cause devastation to crops and native ecosystems worldwide. In New Zealand, Phytophthora agathidicida is threatening the survival of kauri, an endemic, culturally and ecologically important tree species. The current method for detecting P. agathidicida is a soil bating assay that is time-consuming and requires high levels of expertise to assess, thus limiting the analytical sample throughput. Here, we characterized the fatty acid methyl ester (FAME) profile of P. agathidicida. We also compared it with the FAME profile of P. cinnamomi and assessed the efficacy of FAME analysis as a diagnostic tool for detecting the pathogen in soil samples. In FAME analysis, the total fatty acid content is isolated from a sample and converted to FAMEs for analysis, a process that takes less than a day. Unique fatty acid acyl chains can serve as biomarkers for specific organisms. We detected 12 fatty acids in P. agathidicida, two of which (20:4ω6 and 20:5ω3) show promise as potential Phytophthora specific biomarkers. Collectively, these findings advance our fundamental understanding of P. agathidicida biology and provide a promising technique to increase the rate of sample processing and the speed of pathogen detection for P. agathidicida in soil.


2013 ◽  
Author(s):  
Jr Morris ◽  
Shardo Robert W. ◽  
Higgins James ◽  
Cook Kim ◽  
Tanner Rhonda ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0121697 ◽  
Author(s):  
Kirk J. Grubbs ◽  
Jarrod J. Scott ◽  
Kevin J. Budsberg ◽  
Harry Read ◽  
Teri C. Balser ◽  
...  

2017 ◽  
Vol 410 (19) ◽  
pp. 4633-4643 ◽  
Author(s):  
Mohsen Talebi ◽  
Rahul A. Patil ◽  
Leonard M. Sidisky ◽  
Alain Berthod ◽  
Daniel W. Armstrong

2014 ◽  
Vol 91 (11) ◽  
pp. 1967-1974 ◽  
Author(s):  
Subbarao Kandula ◽  
Lucas Stolp ◽  
Michael Grass ◽  
Benjamin Woldt ◽  
Dharma Kodali

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