Characterisation of Eucalyptus rhizospheric communities using fatty acid methyl ester (FAME) profile analysis

2021 ◽  
pp. 1-10
Author(s):  
Chimdi Nwigwe ◽  
Annabel Fossey ◽  
Olga de Smidt
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Profile Analysis ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Fame Profile

scholarly journals The Fatty Acid Methyl Ester (FAME) profile of Phytophthora agathidicida and its potential use as diagnostic tool.

2021 ◽  
Author(s):  
Randy F Lacey ◽  
Blake A Sullivan-Hill ◽  
Julie R Deslippe ◽  
Robert A Keyzers ◽  
Monica L Gerth
Keyword(s):  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Diagnostic Tool ◽  
Fatty Acid Content ◽  
Acid Methyl Ester ◽  
Current Method ◽  
Fame Analysis ◽  
Acid Methyl ◽  
Fame Profile

Phytophthora diseases cause devastation to crops and native ecosystems worldwide. In New Zealand, Phytophthora agathidicida is threatening the survival of kauri, an endemic, culturally and ecologically important tree species. The current method for detecting P. agathidicida is a soil bating assay that is time-consuming and requires high levels of expertise to assess, thus limiting the analytical sample throughput. Here, we characterized the fatty acid methyl ester (FAME) profile of P. agathidicida. We also compared it with the FAME profile of P. cinnamomi and assessed the efficacy of FAME analysis as a diagnostic tool for detecting the pathogen in soil samples. In FAME analysis, the total fatty acid content is isolated from a sample and converted to FAMEs for analysis, a process that takes less than a day. Unique fatty acid acyl chains can serve as biomarkers for specific organisms. We detected 12 fatty acids in P. agathidicida, two of which (20:4ω6 and 20:5ω3) show promise as potential Phytophthora specific biomarkers. Collectively, these findings advance our fundamental understanding of P. agathidicida biology and provide a promising technique to increase the rate of sample processing and the speed of pathogen detection for P. agathidicida in soil.

Evaluation of the Impact of Fatty Acid Methyl Ester (FAME) Contamination on the Thermal Stability of Jet A

2013 ◽  
Author(s):  
Jr Morris ◽  
Shardo Robert W. ◽  
Higgins James ◽  
Cook Kim ◽  
Tanner Rhonda ◽  
...  
Keyword(s):  
Thermal Stability ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
The Impact ◽  
Thermal Stability Of

scholarly journals Unique Honey Bee (Apis mellifera) Hive Component-Based Communities as Detected by a Hybrid of Phospholipid Fatty-Acid and Fatty-Acid Methyl Ester Analyses

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0121697 ◽  
Author(s):  
Kirk J. Grubbs ◽  
Jarrod J. Scott ◽  
Kevin J. Budsberg ◽  
Harry Read ◽  
Teri C. Balser ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Apis Mellifera ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Honey Bee ◽  
Phospholipid Fatty Acid ◽  
Acid Methyl Ester ◽  
Acid Methyl

Branched-chain dicationic ionic liquids for fatty acid methyl ester assessment by gas chromatography

2017 ◽  
Vol 410 (19) ◽  
pp. 4633-4643 ◽  
Author(s):  
Mohsen Talebi ◽  
Rahul A. Patil ◽  
Leonard M. Sidisky ◽  
Alain Berthod ◽  
Daniel W. Armstrong
Keyword(s):  
Gas Chromatography ◽  
Ionic Liquids ◽  
Fatty Acid ◽  
Methyl Ester ◽  
Fatty Acid Methyl Ester ◽  
Acid Methyl Ester ◽  
Acid Methyl ◽  
Dicationic Ionic Liquids ◽  
Branched Chain
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