PCR detection of Fusarium oxysporum f.sp. gladioli race 1, causal agent of Gladiolus yellows disease, from infected corms

2000 ◽  
Vol 49 (1) ◽  
pp. 89-100 ◽  
Author(s):  
de Haan ◽  
Numansen ◽  
Roebroeck ◽  
van Doorn
Keyword(s):  
Fusarium Oxysporum ◽  
Causal Agent ◽  
Pcr Detection ◽  
Race 1

Specific PCR detection of Fusarium oxysporum f. sp. raphani : a causal agent of Fusarium wilt on radish plants

2017 ◽  
Vol 65 (2) ◽  
pp. 133-140 ◽  
Author(s):  
H. Kim ◽  
S.-M. Hwang ◽  
J.H. Lee ◽  
M. Oh ◽  
J.W. Han ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Causal Agent ◽  
Pcr Detection ◽  
Specific Pcr

scholarly journals Identification of Fusarium oxysporum f. sp. lactucae race 1 as the causal agent of lettuce wilt in Brazil

2014 ◽  
Vol 39 (3) ◽  
pp. 197-202 ◽  
Author(s):  
Cléia S. Cabral ◽  
Kátia R. Brunelli ◽  
Hélcio Costa ◽  
Maria Esther de N. Fonseca ◽  
Leonardo S. Boiteux ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Causal Agent ◽  
Race 1

scholarly journals Races and Inoculum Density of Fusarium oxysporum f. sp. niveum in Commercial Watermelon Fields in Maryland and Delaware

Plant Disease ◽  
2003 ◽  
Vol 87 (6) ◽  
pp. 692-698 ◽  
Author(s):  
X. G. Zhou ◽  
K. L. Everts
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Total Population ◽  
Research Field ◽  
Causal Agent ◽  
Inoculum Density ◽  
Differential Cultivars ◽  
Race 1 ◽  
Race 2 ◽  
The Relationship

A survey was conducted to determine races and inoculum density of Fusarium oxysporum f. sp. niveum, the causal agent of Fusarium wilt of watermelon in Maryland and Delaware. Virulence on six differential cultivars was tested for each of 63 isolates of F. oxysporum f. sp. niveum, obtained from 25 commercial watermelon fields. Thirteen isolates (21%) were identified as race 0, 36 isolates (57%) as race 1, and 14 isolates (22%) as race 2. Races 0 and 1 were present in 12 (48%) and 10 (40%) of the fields, respectively. The highly aggressive race 2 was identified from five fields in two counties in Maryland and from one field in Delaware, representing 24% of the fields. Race 2 was copresent with one or two other races. Race 2 (19 isolates) predominated among the 25 isolates obtained from a research field in Maryland. Nineteen commercial fields had inoculum densities of F. oxysporum f. sp. niveum ranging from 100 to 1,200 CFU/g of soil at harvest. Within this range of inoculum densities, >20% incidence of wilt was observed when the susceptible watermelon cv. Sugar Baby was planted in samples of soil collected from these fields. The relationship (P < 0.0001) between inoculum density of F. oxysporum f. sp. niveum (X) and incidence of Fusarium wilt (Y) on Sugar Baby was best described using the monomolecular equation, Y = 1 - exp[-0.0013 (X + 166)]. The ratio of pathogenic to total population of F. oxysporum in the fields linearly increased with increasing inoculum density of F. oxysporum f. sp. niveum (R 2 = 0.4; P < 0.0009).

scholarly journals Draft Genome Sequence of FGL03-6, a Race 1 Strain of Fusarium oxysporum f. sp. conglutinans, the Causal Agent of Cabbage Fusarium Wilt

2018 ◽  
Vol 6 (16) ◽  
pp. e00191-18 ◽  
Author(s):  
Honghao Lv ◽  
Yuhong Yang ◽  
Xing Liu ◽  
Jian Ling ◽  
Zhiyuan Fang ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Genome Sequence ◽  
Draft Genome ◽  
Gc Content ◽  
Causal Agent ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Race 1

ABSTRACT We present here the draft genome sequence of FGL03-6, a race 1 strain of Fusarium oxysporum f. sp. conglutinans, the pathogen causing Fusarium yellows of cabbage. The FGL03-6 genome consists of 414 scaffolds with 61,662,789 bp (GC content, 47.9%) and 9,790 predicted genes.

PCR detection of Gremmeniella abietina, the causal agent of Scleroderris canker of pine

2000 ◽  
Vol 104 (5) ◽  
pp. 527-532 ◽  
Author(s):  
Richard C. Hamelin ◽  
Martin Bourassa ◽  
Jimmy Rail ◽  
Mathieu Dusabenyagasani ◽  
Volker Jacobi ◽  
...  
Keyword(s):  
Causal Agent ◽  
Pcr Detection

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

scholarly journals Outbreak of Fusarium oxysporum f. sp. lycopersici race 3 in commercial fresh-market tomato fields in Rio de Janeiro State, Brazil

2007 ◽  
Vol 25 (3) ◽  
pp. 451-454 ◽  
Author(s):  
Ailton Reis ◽  
Leonardo S Boiteux
Keyword(s):  
Fusarium Oxysporum ◽  
Rio De Janeiro ◽  
Present Report ◽  
Fresh Market ◽  
Solanum Pennellii ◽  
Espírito Santo ◽  
Race 1 ◽  
Serious Disease ◽  
Espirito Santo ◽  
Janeiro State

Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important tomato diseases. In Brazil, all three races were reported, however, race 3 has been so far restricted only to Espírito Santo State. In the present work, seven F. oxysporum isolates obtained from wilted plants of the race 1 and 2-resistant tomato hybrids 'Giovana', 'Carmen' and 'Alambra' in São José de Ubá and Itaocara (Rio de Janeiro State, Brazil) were characterized at race level. Virulence assays were performed using a set of race differential cultivars: 'Ponderosa' (susceptible to all races), 'IPA-5' (resistant to race 1), 'Floradade' (resistant to races 1 and 2), 'BHRS-2,3' (resistant to all three races). Two wild tomato accessions (Solanum pennellii 'LA 716' e S. chilense 'LA 1967') previously reported as resistant to all Brazilian isolates of F. oxysporum f. sp. lycopersici were also evaluated. Isolates from São José de Ubá and Itaocara were highly virulent to 'Ponderosa', 'IPA-5' and 'Floradade'. They were also able to infect a few plants of 'BHRS-2,3', inducing vascular browning and wilt symptoms. Solanum pennellii and S. chilense accessions displayed an extreme (immune-like) resistant response. These results indicated that all seven isolates could be classified as F. oxysporum f. sp. lycopersici race 3, expanding the geographical distribution of this pathogen within Brazil. The hypothesis of transmission via contaminated seeds is reinforced after the present report, which confirms the almost simultaneous outbreak of race 3 in two geographically isolated tomato-growing areas in Brazil (Espirito Santo and Rio de Janeiro). Evaluation of commercial seed lots imported into Brazil for contamination with the pathogen would be necessary in order to avoid nation-wide spread of this serious disease.

scholarly journals Biological control of Fusarium oxysporum causal agent of gladiolus corm rot by streptomycetes

2021 ◽  
Vol 39 (3) ◽  
Author(s):  
Tania Ameyally Rios-Hernández ◽  
Alberto Uc-Varguez ◽  
Zahaed Evangelista-Martínez
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Causal Agent

<em>Fusarium oxysporum</em> causa la pudrición del cormo en gladiolo provocando pérdidas de hasta el 100%. Se seleccionaron aislados de Fusarium a partir de cormos infectados, se identificó morfológica y molecularmente y se seleccionó un aislado a partir de prueba de patogenicidad. Se seleccionó entre 22 aislados de estreptomicetos una cepa que presentó una actividad antagonista del 40% contra <em>Fusarium</em>. Se obtuvo el Extracto Bioactivo (EB) mediante Fermentación en Fase Sólida y se determinó la concentración mínima inhibitoria (MIC) y concentración mínima letal (MLC) por el método de microdilución. Se obtuvo una MIC para el EB de 0.19 mg mL-1 y una MLC de 0.38 mg mL-1, que se confirmó con un ensayo de germinación de microconidios a 8 h, mostrando un porcentaje de inhibición del 17 y 98% para ¼ y ½ de la MIC. Se evaluó el efecto del EB a 1 y 2 MIC’s de concentración contra la pudrición en cormos de gladiolo infectados, obteniendo un efecto protector en los cormos al mantener su dureza después de 15 días, en comparación con el fungicida Carbendazim. Los resultados indican a <em>Streptomyces</em> sp., como un potencial agente de control biológico contra <em>F. oxysporum</em>.

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