Assay development and marker validation for marker assisted selection of Fusarium oxysporum f. sp. niveum race 1 in watermelon

2018 ◽  
Vol 38 (11) ◽  
Author(s):  
Leigh Ann Fall ◽  
Josh Clevenger ◽  
Cecilia McGregor
Keyword(s):  
Fusarium Oxysporum ◽  
Marker Assisted Selection ◽  
Assay Development ◽  
Marker Validation ◽  
Race 1 ◽  
Selection Of

scholarly journals Selection of somaclonal variants of the cultivar ‘Prata-Anã’ for resistance to Fusarium oxysporum f. sp. cubense race 1

2020 ◽  
Vol 42 (6) ◽  
Author(s):  
Mileide dos Santos Ferreira ◽  
Érica Rodrigues de Moura ◽  
Lucymeire Souza Morais Lino ◽  
Edson Perito Amorim ◽  
Janay Almeida dos Santos-Serejo ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Monthly Basis ◽  
Somaclonal Variants ◽  
Banana Tree ◽  
Regenerated Plants ◽  
Race 1 ◽  
Banana Variety ◽  
Selection Of

Abstract The banana tree is one of the most cultivated fruit globally; however, some diseases significantly affect its production, such as Fusarium wilt. The most appropriate measure for controlling this disease in areas with inoculum pressure is the use of resistant cultivars. Therefore, this study aimed to generate banana somaclones of the cultivar ‘Prata-Anã’ resistant to Fusarium wilt by inducing somaclonal variation. ‘Prata-Anã’ stem apexes were established in vitro in MS culture medium and, on a monthly basis, subcultivated in AIA and adenine sulfate supplemented MS medium with added plant regulators: 6-benzylaminopurine (BAP, 4 ml L-1), Thidiazuron (TDZ, 1 ml L-1), and Paclobutrazol (PBZ, 10 ml L-1). The treatments were: T0: no regulator, T1: BAP, T2: TDZ, T3: PBZ, T4: BAP + TDZ, T5: BAP + PBZ, T6: TDZ + PBZ, and T7: BAP + TDZ + PBZ. After the twelfth subculture, the regenerated plants were planted in boxes containing sterile soil infected with Fusarium oxysporum f. sp. cubense, and evaluated after 90 days for resistance to the pathogen. Somaclonal variants T2-1 and T2-2, generated in Treatment 2, with TDZ, were selected as resistant. This result is promising for the launch of a new Fusarium race 1-resistant banana variety.

scholarly journals Marker-Assisted Selection of Tomato Genotypes with the I-2 Gene for Resistance to Fusarium oxysporum f. sp. lycopersici Race 2

Plant Disease ◽  
2007 ◽  
Vol 91 (6) ◽  
pp. 758-762 ◽  
Author(s):  
Choaa A. El Mohtar ◽  
Hagop S. Atamian ◽  
Rachel B. Dagher ◽  
Yusuf Abou-Jawdah ◽  
Melinda S. Salus ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Gene Copy ◽  
Chromosome 11 ◽  
Resistant Varieties ◽  
Race 1 ◽  
Polymerase Chain ◽  
Race 2 ◽  
Environmentally Sound ◽  
Functional Copy ◽  
Selection Of

Fusarium vascular wilt, caused by Fusarium oxysporum f. sp. lycopersici, affects tomatoes worldwide. Development of resistant varieties of tomato would constitute an economically and environmentally sound approach for the management of this disease. Resistance genes to F. oxysporum f. sp. lycopersici race 1 (I-1 gene) and race 2 (I-2 gene) were mapped to chromosome 11. The I-2 gene cluster includes one functional copy and six nonfunctional homologs of the I-2 gene. This report describes the design of primers based on the functional gene copy and the development of a multiplex polymerase chain reaction (PCR)-based method that has the ability to differentiate I-2 genotypes from genotypes without the I-2 gene. In these trials, 39 of the 40 genotypes tested with known reactions to race 2 gave the expected results. The only exception was the cultivar Plum Crimson carrying the I-3 gene for resistance, which confers resistance to F. oxysporum f. sp. lycopersici races 1, 2, and 3. This method was validated in three countries and by bioassays.

scholarly journals Mapping QTL for spike fertility and related traits in two doubled haploid wheat (Triticum aestivum L.) populations

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nicole Pretini ◽  
Leonardo S. Vanzetti ◽  
Ignacio I. Terrile ◽  
Guillermo Donaire ◽  
Fernanda G. González
Keyword(s):  
Doubled Haploid ◽  
Marker Assisted Selection ◽  
Triticum Aestivum L ◽  
Physiological Model ◽  
Yield Potential ◽  
Breeding Programs ◽  
Per Se ◽  
Genomic Regions ◽  
Fertility Traits ◽  
Selection Of

Abstract Background In breeding programs, the selection of cultivars with the highest yield potential consisted in the selection of the yield per se, which resulted in cultivars with higher grains per spike (GN) and occasionally increased grain weight (GW) (main numerical components of the yield). In this study, quantitative trait loci (QTL) for GW, GN and spike fertility traits related to GN determination were mapped using two doubled haploid (DH) populations (Baguette Premium 11 × BioINTA 2002 and Baguette 19 × BioINTA 2002). Results In total 305 QTL were identified for 14 traits, out of which 12 QTL were identified in more than three environments and explained more than 10% of the phenotypic variation in at least one environment. Eight hotspot regions were detected on chromosomes 1A, 2B, 3A, 5A, 5B, 7A and 7B in which at least two major and stable QTL sheared confidence intervals. QTL on two of these regions (R5A.1 and R5A.2) have previously been described, but the other six regions are novel. Conclusions Based on the pleiotropic analysis within a robust physiological model we conclude that two hotspot genomic regions (R5A.1 and R5A.2) together with the QGW.perg-6B are of high relevance to be used in marker assisted selection in order to improve the spike yield potential. All the QTL identified for the spike related traits are the first step to search for their candidate genes, which will allow their better manipulation in the future.

scholarly journals Vegetative Compatibility Groups of Fusarium oxysporum f. sp. lactucae from Lettuce

Plant Disease ◽  
2005 ◽  
Vol 89 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Matias Pasquali ◽  
Flavia Dematheis ◽  
Giovanna Gilardi ◽  
Maria Lodovica Gullino ◽  
Angelo Garibaldi
Keyword(s):  
Fusarium Oxysporum ◽  
Genetic Relatedness ◽  
The United States ◽  
Vegetative Compatibility ◽  
Vegetative Compatibility Group ◽  
Vegetative Compatibility Groups ◽  
Compatibility Group ◽  
Race 1

Fusarium oxysporum f. sp. lactucae, the causal agent of Fusarium wilt of lettuce, has been reported in three continents in the last 10 years. Forty-seven isolates obtained from infected plants and seed in Italy, the United States, Japan, and Taiwan were evaluated for pathogenicity and vegetative compatibility. Chlorate-resistant, nitrate-nonutilizing mutants were used to determine genetic relatedness among isolates from different locations. Using the vegetative compatibility group (VCG) approach, all Italian and American isolates, type 2 Taiwanese isolates, and a Japanese race 1 were assigned to the major VCG 0300. Taiwanese isolates type 1 were assigned to VCG 0301. The hypothesis that propagules of Fusarium oxysporum f. sp. lactucae that caused epidemics on lettuce in 2001-02 in Italian fields might have spread via import and use of contaminated seeds is discussed.

MARKER ASSISTED SELECTION FOR RESISTANCE TO FUSARIUM OXYSPORUM IN THE GREENHOUSE CARNATION

2001 ◽  
pp. 151-156 ◽  
Author(s):  
G. Scovel ◽  
M. Ovadis ◽  
A. Vainstein ◽  
M. Reuven ◽  
Y. Ben-Yephet
Keyword(s):  
Fusarium Oxysporum ◽  
Marker Assisted Selection ◽  
Selection For

scholarly journals Molecular marker-assisted selection of the ae alleles in maize

2010 ◽  
Vol 9 (2) ◽  
pp. 1074-1084
Author(s):  
F. Chen ◽  
S.W. Zhu ◽  
Y. Xiang ◽  
H.Y. Jiang ◽  
B.J. Cheng
Keyword(s):  
Molecular Marker ◽  
Marker Assisted Selection ◽  
Selection Of

scholarly journals Outbreak of Fusarium oxysporum f. sp. lycopersici race 3 in commercial fresh-market tomato fields in Rio de Janeiro State, Brazil

2007 ◽  
Vol 25 (3) ◽  
pp. 451-454 ◽  
Author(s):  
Ailton Reis ◽  
Leonardo S Boiteux
Keyword(s):  
Fusarium Oxysporum ◽  
Rio De Janeiro ◽  
Present Report ◽  
Fresh Market ◽  
Solanum Pennellii ◽  
Espírito Santo ◽  
Race 1 ◽  
Serious Disease ◽  
Espirito Santo ◽  
Janeiro State

Fusarium wilt, caused by three races of Fusarium oxysporum f. sp. lycopersici, is one of the most important tomato diseases. In Brazil, all three races were reported, however, race 3 has been so far restricted only to Espírito Santo State. In the present work, seven F. oxysporum isolates obtained from wilted plants of the race 1 and 2-resistant tomato hybrids 'Giovana', 'Carmen' and 'Alambra' in São José de Ubá and Itaocara (Rio de Janeiro State, Brazil) were characterized at race level. Virulence assays were performed using a set of race differential cultivars: 'Ponderosa' (susceptible to all races), 'IPA-5' (resistant to race 1), 'Floradade' (resistant to races 1 and 2), 'BHRS-2,3' (resistant to all three races). Two wild tomato accessions (Solanum pennellii 'LA 716' e S. chilense 'LA 1967') previously reported as resistant to all Brazilian isolates of F. oxysporum f. sp. lycopersici were also evaluated. Isolates from São José de Ubá and Itaocara were highly virulent to 'Ponderosa', 'IPA-5' and 'Floradade'. They were also able to infect a few plants of 'BHRS-2,3', inducing vascular browning and wilt symptoms. Solanum pennellii and S. chilense accessions displayed an extreme (immune-like) resistant response. These results indicated that all seven isolates could be classified as F. oxysporum f. sp. lycopersici race 3, expanding the geographical distribution of this pathogen within Brazil. The hypothesis of transmission via contaminated seeds is reinforced after the present report, which confirms the almost simultaneous outbreak of race 3 in two geographically isolated tomato-growing areas in Brazil (Espirito Santo and Rio de Janeiro). Evaluation of commercial seed lots imported into Brazil for contamination with the pathogen would be necessary in order to avoid nation-wide spread of this serious disease.

scholarly journals Development of KASP Markers for Germplasm Characterization and Fingerprinting Identification of Broccoli in China

2020 ◽  
Author(s):  
Yusen Shen ◽  
Jiansheng Wang ◽  
Huifang Yu ◽  
Xiaoguang Sheng ◽  
Zhenqing Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Marker Assisted Selection ◽  
Genetic Relationships ◽  
Cost Effective ◽  
Germplasm Characterization ◽  
2D Barcode ◽  
New Type ◽  
Generation Sequencing ◽  
Kasp Markers ◽  
Selection Of

Abstract Background: Broccoli (Brassica oleracea var. italica) is a vegetable widely cultivated in China. Many new-type broccoli cultivars were bred and developed by Chinese breeders during the recent three decades. However, the broccoli cultivar nomenclature and detailed information of genetic relationships among broccoli germplasms are unclear. Results: The present study identified millions of SNPs by next-generation sequencing of 23 representative broccoli lines. Through several steps of selection, 100 SNPs were successfully converted into KASP markers, and used to evaluate the genetic diversity, genetic relationship, and population structure of 392 broccoli accessions, which represent the mainly broccoli breeding materials in China. The initial, introduced and improved accessions were well clustered, though some accessions were overlapped between groups, probably reflecting the fact that breeding activities led to genetic similarities. To make the KASP genotyping more efficient and cost-effective, 25 of the 100 KASPs were selected for fingerprinting of all accessions, and the 2D barcode contained fingerprinting information were generated for elite varieties. Conclusion: The KASP markers developed in this study provided an efficient way for germplasm characterization, DNA fingerprinting, seed purity identification, and marker-assisted selection of broccoli in China.

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