Photochemical Inactivation of Bacteria and HIV in Buffy-Coat-Derived Platelet Concentrates under Conditions That Preserve in vitro Platelet Function

Quality control of platelet concentrates (PC) is an important prerequisite for good transfusion praxis. However, direct measurement of platelet function is complex, since available methods (e.g. aggregometry, serotonin release) are time consuming and require special equipment. Therefore a test system is needed, which is easy to handle, fast, and achieves reliable results. The present paper compares the results of conventional platelet function tests with those of a modified in-vitro bleeding test (IVBT) (Thrombostat 4000) in liquid-stored and cryopreserved PCs. A high correlation between aggregometry, serotonin release, GMP 140 expression upon stimulation, and IVBT was demonstrated. Therefore IVBT seems to be a good alternative to the conventional platelet function tests for quality control of PCs. In addition, a good correlation between the results of IVBT of patients’ blood after PC transfusion and IVBT of patients blood before transfusion supplemented with platelets of the respective PC could be found. Therefore IVBT seems to be able to predict PC transfusion success. However, since these data were obtained in a small sample undergoing bone marrow transplantation, further studies are needed to verify this hypothesis.

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In vitro platelet function of platelet concentrates transfused to high risk cardiac surgery patients as determined bedside by the Multiplate impedance aggregometry

European Journal of Anaesthesiology ◽

10.1097/00003643-200805001-00264 ◽

2008 ◽

Vol 25 (Sup 44) ◽

pp. 83

Author(s):

C. Jambor ◽

C. Weber ◽

K. van Beesel ◽

M. Mueller ◽

B. Zwissler

Keyword(s):

Cardiac Surgery ◽

High Risk ◽

Platelet Function ◽

Platelet Concentrates ◽

Impedance Aggregometry

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Normal platelet function in platelet concentrates requires non-platelet cells: a comparative in vitro evaluation of leucocyte-rich (type 1a) and leucocyte-poor (type 3b) platelet concentrates

BMJ Open Sport & Exercise Medicine ◽

10.1136/bmjsem-2015-000071 ◽

2016 ◽

Vol 2 (1) ◽

pp. e000071 ◽

Cited By ~ 19

Author(s):

William R Parrish ◽

Breana Roides ◽

Julia Hwang ◽

Michael Mafilios ◽

Brooks Story ◽

...

Keyword(s):

Platelet Function ◽

Platelet Concentrates ◽

In Vitro Evaluation ◽

Normal Platelet

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In vitro study of platelet function confirms the contribution of the ultraviolet B (UVB) radiation in the lesions observed in riboflavin/UVB-treated platelet concentrates

Transfusion ◽

10.1111/trf.13123 ◽

2015 ◽

Vol 55 (9) ◽

pp. 2219-2230 ◽

Cited By ~ 25

Author(s):

Mélanie Abonnenc ◽

Giona Sonego ◽

David Crettaz ◽

Alessandro Aliotta ◽

Michel Prudent ◽

...

Keyword(s):

Platelet Function ◽

In Vitro Study ◽

Ultraviolet B ◽

Vitro Study ◽

Uvb Radiation ◽

Platelet Concentrates

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Storage of Platelet Concentrates from Overnight-Stored Blood and Overnight-Stored Buffy Coat: In vitro Studies

Vox Sanguinis ◽

10.1159/000462920 ◽

1995 ◽

Vol 68 (3) ◽

pp. 160-163

Author(s):

Z. Ràcz ◽

C. Baróti

Keyword(s):

Buffy Coat ◽

In Vitro Studies ◽

Platelet Concentrates ◽

Stored Blood

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Photochemical inactivation of pathogenic bacteria in human platelet concentrates

Blood ◽

10.1182/blood.v83.9.2698.2698 ◽

1994 ◽

Vol 83 (9) ◽

pp. 2698-2706 ◽

Cited By ~ 59

Author(s):

L Lin ◽

H Londe ◽

JM Janda ◽

CV Hanson ◽

L Corash

Keyword(s):

Platelet Function ◽

Pathogenic Bacteria ◽

Uv Light ◽

Platelet Concentrates ◽

Gram Positive ◽

Plasma Protein Concentration ◽

Transfusion Therapy ◽

Gram Negative ◽

Gram Negative Organisms

Abstract Platelet concentrates (PC) may be infrequently contaminated with low levels of bacteria that can cause septicemia and death in patients receiving transfusion therapy. We evaluated the efficacy of a photochemical decontamination (PCD) technique using 8-methoxypsoralen (8-MOP) and long wavelength UV light (UVA) to inactivate bacteria in standard therapeutic PC. Twelve phylogenetically distinct pathogenic bacteria, 5 gram-positive and 7 gram-negative organisms, were seeded into PC to a final challenge dose ranging from 10(5) to 10(7) colony- forming units (CFU)/mL. Contaminated PC were treated with 8-MOP (5 micrograms/mL) and 5 J/cm2 of UVA, a PCD treatment regimen found to adequately preserve in vitro platelet function. Greater than 10(5) CFU/mL of all 5 gram-positive (Staphylococcus aureus, Streptococcus epidermidis, Streptococcus pyogenes, Listeria monocytogenes, and Corynebacterium minutissimum) and 2 of the gram-negative (Escherichia coli and Yersinia enterocolitica) organisms were inactivated. The remaining 5 gram-negative organisms were more resistant, with less than 10(1) to 10(3.7) CFU/mL inactivated under these conditions. The inactivation efficiency for this resistant group of gram-negative organisms was improved when PC were resuspended in a synthetic storage medium with reduced plasma protein concentration (15%) and an increased 8-MOP concentration (23.4 micrograms/mL). Illumination with 3 J/cm2 of UVA in this system inactivated greater than 10(5) CFU/mL of 4 resistant gram-negative organisms (Salmonella choleraesuis, Enterobacter cloacae, Serratia marcescens, and Klebsiella pneumoniae) and 10(4.1) CFU/mL of the most resistant gram-negative organism (Pseudomonas aeruginosa). This level of PCD treatment did not adversely affect in vitro platelet function. These results demonstrate that PCD using 8-MOP (5 to 23.4 micrograms/mL) effectively inactivated high levels of pathogenic bacteria in PC with adequate preservation of in vitro platelet properties.

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