scholarly journals Synergism between a half-site and an imperfect estrogen-responsive element, and cooperation with COUP-TFI are required for estrogen receptor (ER) to achieve a maximal estrogen-stimulation of rainbow trout ER gene

1999 ◽  
Vol 259 (1-2) ◽  
pp. 385-395 ◽  
Author(s):  
Fabrice G. Petit ◽  
, Raphaël Métivier ◽  
, Yves Valotaire ◽  
Farzad Pakdel
Keyword(s):  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Estrogen Responsive Element ◽  
Responsive Element ◽  
Estrogen Stimulation ◽  
Half Site ◽  
Stimulation Of

Characterization of an estrogen-responsive element implicated in regulation of the rainbow trout estrogen receptor gene

1995 ◽  
Vol 15 (1) ◽  
pp. 37-47 ◽  
Author(s):  
Y Le Dréan ◽  
G Lazennec ◽  
L Kern ◽  
D Saligaut ◽  
F Pakdel ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Rainbow Trout ◽  
Receptor Gene ◽  
Expression System ◽  
Consensus Sequence ◽  
Gene Promoter ◽  
Dnase I Footprinting ◽  
Estrogen Responsive Element ◽  
Responsive Element ◽  
Simplex Virus

ABSTRACT We previously reported that the expression of the rainbow trout estrogen receptor (rtER) gene is markedly increased by estradiol (E2). In this paper, we have used transient transfection assays with reporter plasmids expressing chloramphenicol acetyl transferase (CAT), linked to 5′ flanking regions of the rtER gene promoter, to identify cis-elements responsible for E2 inducibility. Deletion analysis localized an estrogen-responsive element (ERE), at position +242, with one mutation on the first base compared with the consensus sequence. This element confers estrogen responsiveness to CAT reporter linked to both the herpes simplex virus thymidine kinase promoter and the homologous rtER promoter. Moreover, using a 0·2 kb fragment of the rtER promoter encompassing the ERE and the rtER DNA binding domain obtained from a bacterial expression system, DNase I footprinting experiments demonstrated a specific protection covering 20 bp (+240/+260) containing the ERE sequence. Based on these studies, we believe that this ERE sequence, identified in the rtER gene promoter, may be a major cis-acting element involved in the regulation of the gene by estrogen.

scholarly journals Estrogen Stimulation of Kiss1 Expression in the Medial Amygdala Involves Estrogen Receptor-α But Not Estrogen Receptor-β

Endocrinology ◽  
2016 ◽  
Vol 157 (10) ◽  
pp. 4021-4031 ◽  
Author(s):  
Shannon B. Z. Stephens ◽  
Navdeep Chahal ◽  
Nagambika Munaganuru ◽  
Ruby A. Parra ◽  
Alexander S. Kauffman
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Receptor Α ◽  
Estrogen Receptor Β ◽  
Medial Amygdala ◽  
Estrogen Stimulation ◽  
Stimulation Of

scholarly journals A 13 bp palindrome is a functional estrogen responsive element and interacts specifically with estrogen receptor

1988 ◽  
Vol 16 (2) ◽  
pp. 647-663 ◽  
Author(s):  
Ludger Klein-Hitpass ◽  
Gerhart U. Ryffel ◽  
Ellen Heitlinger ◽  
Andrew C.B. Cato
Keyword(s):  
Estrogen Receptor ◽  
Estrogen Responsive Element ◽  
Responsive Element

Measurement of vitellogenin from rainbow trout by rocket immunoelectrophoresis: application to the kinetic analysis of estrogen stimulation in the male

1985 ◽  
Vol 63 (9) ◽  
pp. 982-987 ◽  
Author(s):  
Jean Louis Maitre ◽  
Catherine Le Guellec ◽  
Stephane Derrien ◽  
Martin Tenniswood ◽  
Yves Valotaire
Keyword(s):  
Rainbow Trout ◽  
Salmo Gairdneri ◽  
Immature Female ◽  
External Appearance ◽  
Seasonal Regulation ◽  
Low Levels ◽  
Estrogen Stimulation ◽  
Different Levels ◽  
Estradiol Levels ◽  
Stimulation Of

The study of the seasonal regulation of vitellogenesis in rainbow trout (Salmo gairdneri) is hampered by two features of the system which are not seen in species such as Xenopus. First, it is impossible to sex immature trout by external appearance and, secondly, the quantitation of the very low levels of vitellogenin in previtellogenic serum is technically difficult and tedious. We describe the preparation of a specific, sensitive anti-vitellogenin antibody and the use of this antibody in a rocket immunoelectrophoresis system to measure serum vitellogenin. The sensitivity of the assay is such that, using only 2 μL of serum, it is possible to detect vitellogenin at levels of 10 μg/mL, making this assay extremely useful for selecting immature female trout for further studies on the basis of the presence of vitellogenin. Using this system we have also measured the response of individual male trout to stimulation with different levels of estradiol, and we have shown that it is possible to measure the stimulation of vitellogenin by estradiol levels equivalent to those seen during the previtellogenic phase of the reproductive cycle in females. This simple assay system thus alleviates two of the major hurdles in studying vitellogenesis in trout.

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