Genetic relationships between South African wheat cultivars as measured by gliadin banding patterns

2000 ◽  
Vol 119 (3) ◽  
pp. 280-282 ◽  
Author(s):  
M. T. Labuschagne ◽  
A. M. Swardt ◽  
C. D. Viljoen
2013 ◽  
Vol 94 (10) ◽  
pp. 2338-2351 ◽  
Author(s):  
Kristy Offerman ◽  
Olivia Carulei ◽  
Tertius A. Gous ◽  
Nicola Douglass ◽  
Anna-Lise Williamson

Thirteen novel avipoxviruses were isolated from birds from different regions of South Africa. These viruses could be divided into six groups, according to gross pathology and pock appearance on chick chorioallantoic membranes (CAMs). Histopathology revealed distinct differences in epidermal and mesodermal cell proliferation, as well as immune cell infiltration, caused by the different avipoxviruses, even within groups of viruses causing similar CAM gross pathology. In order to determine the genetic relationships among the viruses, several conserved poxvirus genetic regions, corresponding to vaccinia virus (VACV) A3L (fpv167 locus, VACV P4b), G8R (fpv126 locus, VLTF-1), H3L (fpv140 locus, VACV H3L) and A11R–A12L (fpv175–176 locus) were analysed phylogenetically. The South African avipoxvirus isolates in this study all grouped in clade A, in either subclade A2 or A3 of the genus Avipoxvirus and differ from the commercial fowlpox vaccines (subclade A1) in use in the South African poultry industry. Analysis of different loci resulted in different branching patterns. There was no correlation between gross morphology, histopathology, pock morphology and phylogenetic grouping. There was also no correlation between geographical distribution and virus phenotype or genotype.


Biologia ◽  
2011 ◽  
Vol 66 (2) ◽  
Author(s):  
Sanghamitra Samantaray ◽  
Tarun Patel ◽  
K. Geetha ◽  
Satyabrata Maiti

AbstractConservation of identified germplasm is an important component for efficient and effective management of plant genetic resources. Since Chlorophytum species are important medicinal plants, studies were carried out for identification and establish genetic relationships in three species of Chlorophytum and two high yielding genotypes of Chlorophtum borivilianum using RAPD markers. Out of one hundred primers tested, 47 decamers amplified a total of 454 distinct bands ranging from 0.25–3.0 kbp to identify and to evaluate genetic relationships between and among three species of Chlorophytum and two genotypes of Chlorophtum borivilianum. The cluster analysis indicated that three species of Chlorophytum and two genotypes (NRCCB-1 and NRCCB-2) of C. borivilianum formed two major clusters. The first major cluster constituted C. arundinaceum and C. tuberosum, and the second major cluster composed of two subclusters; the first subcluster represented NRCB-1 and NRCB-2 where as the second subcluster represented C. borivilianum. Thus, the RAPD markers have the potential for identification and characterization of genetic relatedness among the species and genotypes. C. borivilianum along with two genotypes also showed similar banding patterns which could be chosen as candidate markers for differentiating the other two species such as C. arundinaceum and C. tuberosum. This would helpful for breeding programmes and provides an important input in conservation biology.


2000 ◽  
Vol 13 (3) ◽  
pp. 409 ◽  
Author(s):  
I. J. Radford ◽  
P. Muller ◽  
S. Fiffer ◽  
P. W. Michael

An isozyme and morphological study of Senecio madagascariensis Poir. and closely related species in Australia, South Africa and Madagascar was used to investigate the most likely region of origin for Australian plants known as fireweed. Collections of seed and voucher specimens were made in New South Wales, KwaZulu-Natal, East and West Cape Provinces and southern Madagascar and specimens classified according to recognised taxa or descriptive variants. Plants were characterised by using isozyme analyses and morphological observations of both voucher specimens and achenes. Australian fireweed populations were found to be most closely related to populations of S. madagascariensis from KwaZulu-Natal in South Africa, and to be more distantly related to populations from East and West Cape Provinces in South Africa and from Madagascar. The implications of these findings are discussed in relation to efforts to locate host-specific biological control agents for fireweed.


2016 ◽  
Vol 141 (5) ◽  
pp. 520-526 ◽  
Author(s):  
Qijing Zhang ◽  
Dajun Gu

To improve the efficiency of breeding programs for Prunus rootstock hybrids in China, we analyzed the subgenus status and relationship of 10 Chinese rootstock species, by using 24 sets of simple sequence repeat (SSR) primers. The SSR banding patterns and phylogenetic analysis indicated that subgenus Cerasus is more closely related to subgenus Prunophora than to subgenus Amygdalus, and that subgenus Lithocerasus is more closely related to subgenus Prunophora and subgenus Amygdalus than to subgenus Cerasus. In addition, Prunus triloba was more closely related to Prunus tomentosa than to the members of subgenus Amygdalus. Therefore, we suggest that P. tomentosa and P. triloba should be assigned to the same group, either to subgenus Lithocerasus or Prunophora, and we also propose potential parent combinations for future Prunus rootstock breeding.


2000 ◽  
Vol 66 (4) ◽  
pp. 1362-1383 ◽  
Author(s):  
Yu-Sheng Chen ◽  
Antonel Olckers ◽  
Theodore G. Schurr ◽  
Andreas M. Kogelnik ◽  
Kirsi Huoponen ◽  
...  

2014 ◽  
Vol 33 (2) ◽  
pp. 127-131 ◽  
Author(s):  
M.T. Labuschagne ◽  
N. Mkhatywa ◽  
B. Wentzel ◽  
E. Johansson ◽  
A. van Biljon

2018 ◽  
Vol 6 (3) ◽  
pp. 95-109
Author(s):  
Manal Eid

The present study was carried out to conduct drought tolerance in three wheat cultivars including susceptible (Gemmiza7) and tolerant (Sakha93 and Sahel1). Molecular characterization was done by 26 SSR markers located on chromosome7 which was associated with drought tolerance in many previous studies. 26 SSR markers were polymorphic and thus showed 100% polymorphism. The number of alleles per locus varied from 2 to 3 alleles with an average (2.62). The polymorphism information content (PIC) value ranged from 0.34 to 0.59, with a mean of 0.51. The discrimination power (Dp) value ranged between 0.67 and 0.78 with an average of 0.71 per locus and Heterozygosity (He) value varied from 0.44 to 0.67 with an average of 0.59. The genetic relationships estimated by the polymorphism of SSR markers revealed a greater level of genetic variability in wheat cultivars of wide adaptability and applicability. Whereas an average of combined probability value for the SSR markers was 6.15 x 10-16, suggests the capability of the marker system to distinguish identity and purity of wheat cultivars. In addition to the SSR markers revealed various bands that were either absent or present within tolerant cultivars (Sakha93 and Sahel1) which were altogether absent in susceptible cultivar (Gemmiza7). Also, SSRs of diagnostic and curatorial importance were discerned as ‘stand-alone’ molecular descriptors for barcoding the application of DNA sequences of standardized genetic markers for the identification of wheat cultivars. However, the genetic information in this study could provide useful information to address breeding programs and germplasm resource management.


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