Cerebral Dura Mater and Cephalalgia: Relationships Between Mast Cells and Catecholaminergic Nerve Fibers in the Rat

The aim of the present study was to examine whether mast cells have the same variations as the related catecholaminergic nerve fibers. Chemical sympathectomy or surgical removal of right superior cervical ganglion induced a rapid decrease of fluorescence in both nerve fibers and mast cells, as confirmed by quantitative analysis (nerve fibers 19±1.1 vs 1.3±0.6; mast cell 10.8±1.9 vs 2.1±0.3). The results of quantitative analysis after nerve fiber stimulation (electrical), however, showed an increase of the fluorescence in both the nerve fibers and the mast cells (nerve fibers 43.4 ±2.4; mast cells 18.6 ±1.6). Moreover, we found that the basal zone is more innervated (regarding catecholaminergic nerve fibers) than the apical one, and that the fluorescence level decreases passing from the vasal zone to the perivasal and intervasal zones. Further studies are needed in order to clarify the role of fluorescent nerve fibers and mast cells of cerebral dura mater in cephalalgia.

Download Full-text

Functional relationships between sensory nerve fibers and mast cells of dura mater in normal and inflammatory conditions

Neuroscience ◽

10.1016/s0306-4522(96)00488-5 ◽

1997 ◽

Vol 77 (3) ◽

pp. 829-839 ◽

Cited By ~ 79

Author(s):

V Dimitriadou ◽

A Rouleau ◽

M.D Trung Tuong ◽

G.J.F Newlands ◽

H.R.P Miller ◽

...

Keyword(s):

Mast Cells ◽

Dura Mater ◽

Sensory Nerve ◽

Nerve Fibers ◽

Functional Relationships ◽

Inflammatory Conditions

Download Full-text

Abstract 15228: Role of Sympathetic Innervation in the Pathogenesis of Abdominal Aortic Aneurysm

Circulation ◽

10.1161/circ.142.suppl_3.15228 ◽

2020 ◽

Vol 142 (Suppl_3) ◽

Author(s):

Bin JIANG ◽

Yugang Liu ◽

Guillermo A Ameer

Keyword(s):

Abdominal Aortic Aneurysm ◽

Aortic Aneurysm ◽

Sympathetic Innervation ◽

Nerve Fibers ◽

Elastic Fibers ◽

Surgical Removal ◽

Infrarenal Aorta ◽

Abdominal Aortic ◽

Nerve Bundles

Introduction: The objective of this study is to understand the role of neurological factors, specifically those from the perivascular sympathetic nervous system (SNS), on the initiation and development of Abdominal Aortic Aneurysm (AAA). Hypothesis: We hypothesize that the formation of AAA is associated with the loss of perivascular SNS-induced vasoconstriction specific to the aneurysm region. Methods: We developed a rat Abdominal Aortic Denervation (AAD) model, where the infrarenal aorta of Spauge Dawley rats was denervated with surgical removal of nerve fibers and chemical denervation with 10% phenol ( Figure. A ). A sham control group was included where the infrarenal aorta was treated with PBS. The arteries were harvested at 1 month after the surgeries for histological assessment. Results: The denervated aortas exhibited significant thinning of the aortic wall including the media and the adventitia, compared to the sham controls ( Figure. B ). Moreover, degradation of elastin, demonstrated by the fragmentation of elastic fibers and the decreased number of lamellar units, was also observed in the dennervated aortas in comparison to the sham controls. While the control aortas were well innervated with perivascular nerve bundles adjacent to the adventitia, no nerves were found surrounding the denervated aortas, suggesting successful denervation. Conclusions: We generated an AAD model that could be used for mechanistic understanding and therapeutic development of AAA. The preliminary data suggest a direct link between the lack of aortic sympathetic innervation and AAA formation. Long-term studies are currently underway to further characterize changes in the aortic walls after sympathetic denervation. Figure. (A) Illustration of the denervated region on the rat infrarenal aorta. ( B ) Histological staining of control and denervated rat abdominal aortas at 1 month after surgery. Yellow stars: para-aortic nerve bundles. Scale bar = 200 μm.

Download Full-text

Influence of the Sympathetic Nervous System as Well as Trigeminal Sensory Fibres on Rat Dural Mast Cells

Cephalalgia ◽

10.1046/j.1468-2982.1991.1105215.x ◽

1991 ◽

Vol 11 (5) ◽

pp. 215-222 ◽

Cited By ~ 33

Author(s):

JT Keller ◽

RVW Dimlich ◽

M Zuccarello ◽

L Lanker ◽

TA Strauss ◽

...

Keyword(s):

Nervous System ◽

Mast Cells ◽

Sympathetic Nervous System ◽

Dura Mater ◽

Autonomic Nerve ◽

Trigeminal System ◽

Vascular Headache ◽

Cervical Ganglion ◽

Sympathetic Nervous ◽

Potential Clinical Impact

The dura mater has attracted considerable attention as an exquisitely sensitive tissue implicated as playing a role in various cephalalgias including vascular headache. Because of the potential clinical impact of the relationship/interaction of neural elements and mast cells the influence of sensory and autonomic nerve fibres on mast cells of the rat dura mater was studied. The trigeminal or superior cervical ganglion was electrically stimulated and the mast cells were examined. Wholemount supratentorial dural preparations were stained using berberine sulphate and the number of mast cells with intact vs dispersed granules counted. Unilateral stimulation of either ganglia resulted in a statistically significant increase in the percentage of mast cells with dispersed granules ipsilateral to the side of stimulation. These results support our idea that in addition to the trigeminal system the sympathetic nervous system must be considered as playing a role in the oedema pathophysiology of vascular headache.

Download Full-text

Role Of Cgrp In Sensitization Of Dura Mater

The Scientific World JOURNAL ◽

10.1100/tsw.2001.428 ◽

2001 ◽

Vol 1 ◽

pp. 20-20

Author(s):

K. Messlinger

Keyword(s):

Dura Mater ◽

Trigeminal Nerve ◽

Plasma Levels ◽

Nerve Fibers ◽

Considerable Proportion ◽

Trigeminovascular System ◽

Dura Mater Encephali ◽

Related Peptide ◽

Calcitonin Gene

The mammalian dura mater encephali is richly supplied by trigeminal nerve fibers, a considerable proportion of which contains calcitonin gene-related peptide (CGRP). As plasma levels of CGRP are increased in some forms of headaches, the question is in which way CGRP is involved in nociceptive mechanisms within the peripheral and the central trigeminovascular system.

Download Full-text

Tryptophan hydroxylase can be present in mast cells and nerve fibers of the rat dura mater but only mast cells contain serotonin

Neuroscience Letters ◽

10.1016/0304-3940(94)90781-1 ◽

1994 ◽

Vol 182 (2) ◽

pp. 133-137 ◽

Cited By ~ 18

Author(s):

Pierrette Mathiau ◽

Norbert Bakalara ◽

Pierre Aubineau

Keyword(s):

Mast Cells ◽

Dura Mater ◽

Tryptophan Hydroxylase ◽

Nerve Fibers

Download Full-text

Overexpression of NGF in mouse urothelium leads to neuronal hyperinnervation, pelvic sensitivity, and changes in urinary bladder function

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00367.2009 ◽

2010 ◽

Vol 298 (3) ◽

pp. R534-R547 ◽

Cited By ~ 100

Author(s):

Birthe Schnegelsberg ◽

Tung-Tien Sun ◽

Gary Cain ◽

Anindya Bhattacharya ◽

Philip A. Nunn ◽

...

Keyword(s):

Mast Cells ◽

Urinary Bladder ◽

Transgenic Mice ◽

Bladder Dysfunction ◽

Sympathetic Neurons ◽

Nerve Fibers ◽

Bladder Function ◽

Sensory Afferents ◽

Urinary Bladder Dysfunction

NGF has been suggested to play a role in urinary bladder dysfunction by mediating inflammation, as well as morphological and functional changes, in sensory and sympathetic neurons innervating the urinary bladder. To further explore the role of NGF in bladder sensory function, we generated a transgenic mouse model of chronic NGF overexpression in the bladder using the urothelium-specific uroplakin II (UPII) promoter. NGF mRNA and protein were expressed at higher levels in the bladders of NGF-overexpressing (NGF-OE) transgenic mice compared with wild-type littermate controls from postnatal day 7 through 12–16 wk of age. Overexpression of NGF led to urinary bladder enlargement characterized by marked nerve fiber hyperplasia in the submucosa and detrusor smooth muscle and elevated numbers of tissue mast cells. There was a marked increase in the density of CGRP- and substance P-positive C-fiber sensory afferents, neurofilament 200-positive myelinated sensory afferents, and tyrosine hydroxylase-positive sympathetic nerve fibers in the suburothelial nerve plexus. CGRP-positive ganglia were also present in the urinary bladders of transgenic mice. Transgenic mice had reduced urinary bladder capacity and an increase in the number and amplitude of nonvoiding bladder contractions under baseline conditions in conscious open-voiding cystometry. These changes in urinary bladder function were further associated with an increased referred somatic pelvic hypersensitivity. Thus, chronic urothelial NGF overexpression in transgenic mice leads to neuronal proliferation, focal increases in urinary bladder mast cells, increased urinary bladder reflex activity, and pelvic hypersensitivity. NGF-overexpressing mice may, therefore, provide a useful transgenic model for exploring the role of NGF in urinary bladder dysfunction.

Download Full-text

Trigeminal sensory fiber stimulation induces morphological changes reflecting secretion in rat dura mater mast cells

Neuroscience ◽

10.1016/0306-4522(91)90253-k ◽

1991 ◽

Vol 44 (1) ◽

pp. 97-112 ◽

Cited By ~ 198

Author(s):

V. Dimtriadou ◽

M.G. Buzzi ◽

M.A. Moskowitz ◽

T.C. Theoharides

Keyword(s):

Mast Cells ◽

Dura Mater ◽

Morphological Changes ◽

Sensory Fiber ◽

Fiber Stimulation

Download Full-text

Ultrastructural evidence for mast cell-macrophage interrelationships in the dura mater of the rat: Infrequent mast cell-nerve associations

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100159308 ◽

1990 ◽

Vol 48 (3) ◽

pp. 352-353

Author(s):

Ruth V.W. Dimlich

Keyword(s):

Mast Cell ◽

Mast Cells ◽

Dura Mater ◽

Spatial Relationship ◽

Potassium Phosphate ◽

Plasma Extravasation ◽

Close Spatial Relationship ◽

Male Wistar Rats ◽

Headache Pain ◽

Relationship Of

Mast cells in the dura mater of the rat may play a role in cerebral pathologies including neurogenic inflammation (vasodilation; plasma extravasation) and headache pain . As has been suggested for other tissues, dural mast cells may exhibit a close spatial relationship to nerves. There has been no detailed ultrastructural description of mast cells in this tissue; therefore, the goals of this study were to provide this analysis and to determine the spatial relationship of mast cells to nerves and other components of the dura mater in the rat.Four adult anesthetized male Wistar rats (290-400 g) were fixed by perfusion through the heart with 2% glutaraldehyde and 2.8% paraformaldehyde in a potassium phosphate buffer (pH 7.4) for 30 min. The head of each rat was removed and stored in fixative for a minimum of 24 h at which time the dural coverings were removed and dissected into samples that included the middle meningeal vasculature. Samples were routinely processed and flat embedded in LX 112. Thick (1 um) sections from a minimum of 3 blocks per rat were stained with toluidine blue (0.5% aqueous).

Download Full-text