RESEARCH ON ACCURATE TEMPERATURE CONTROL ALGORITHM OF AGAROSE GEL SOL APPARATUS FOR NUCLEIC ACID DETECTION

Author(s):  
S. Zhang ◽  
X. Lang ◽  
A. TuErHong
1995 ◽  
Vol 81 (3) ◽  
pp. 191-196
Author(s):  
Takashi ODA ◽  
Yoshitaka KONDO ◽  
Seiji KONISHI ◽  
Hironori MURAKAMI ◽  
Masayoshi SUEHIRO ◽  
...  

Author(s):  
Paul R. van der Meer ◽  
Gerard C. M. Meijer ◽  
Michiel J. Vellekoop ◽  
Harry M. M. Kerkvliet ◽  
Ton J. J. van den Boom

1979 ◽  
Vol 22 (87) ◽  
pp. 389-391
Author(s):  
V. I. Morgan

AbstractA temperature controller is described which uses a thermistor sensor and electrical heating to maintain the temperature of a small bath of fluid to within a few millidegrees.


2011 ◽  
Vol 383-390 ◽  
pp. 1215-1218
Author(s):  
Wen Gang Chen ◽  
Xiao Jie Song ◽  
Wei Liu

Aiming to solve the problems of inaccuracy in temperature control and the slow reaction in condensing system in the automated distillation analyzer, an approach which combining electrical fan and thermoelectric cooler was put forward, using STM32 as the control unit; accurate temperature control during the distillation process was realized, data collection and processing can be completed by the control unit, and the wireless module can accomplish communication and control the analyzer under the instructions of computer. With this temperature control method, the automation and test accuracy of distillation analyzer can be enhanced, thus ensuring better ratio of performance-to-price and control performance.


Author(s):  
Z. Jay Cao ◽  
Aaron J. Knobloch ◽  
Wei-Cheng Tian ◽  
Stacey J. Kennerly ◽  
Nannan Chen ◽  
...  

Two key challenges to portable gas chromatography are reducing preconcentrator power consumption and accurate temperature control of adsorbent. This paper presents the results of thermal modeling performed to optimize a microfabricated preconcentrator based on a silicon microhotplate and utilizing Metal Organic Framework (MOF) adsorbents. From this modeling, two design changes are presented that reduce the power consumption by 1.5 W and reduce temperature variation across the microhotplate by 50%.


1969 ◽  
Vol 8 (2) ◽  
pp. 130-133 ◽  
Author(s):  
C. W. van der Wal ◽  
C. J. Nederveen ◽  
G. A. Schwippert

2021 ◽  
Vol 8 ◽  
Author(s):  
Caiyun Huo ◽  
Donghai Li ◽  
Zhenguo Hu ◽  
Guiping Li ◽  
Yanxin Hu ◽  
...  

Avibacterium paragallinarum, the pathogen of infectious coryza, caused a highly contagious respiratory disease that poses a serious threat to chickens. Hence, it is necessary to do diagnostic screening for Av. paragallinarum. Existing technologies have been used for Av. paragallinarum testing, which, however, have some drawbacks such as time consuming and expensive that require well-trained personnel and sophisticated infrastructure, especially when they are limitedly feasible in some places for lack of resources. Nucleic acid hybridization-based lateral flow assay (LFA) is capable of dealing with these drawbacks, which is attributed to the advantages, such low cost, rapid, and simple. However, nucleic acid determination of Av. paragallinarum through LFA method has not been reported so far. In this study, we developed a novel LFA method that employed gold nanoparticle probes to detect amplified Av. paragallinarum dsDNA. Compared with agarose gel electrophoresis, this LFA strip was inexpensive, simple- to- use, and time- saving, which displayed the visual results within 5–8 min. This LFA strip had higher sensitivity that achieved the detection limit of 101 CFU/ml compared with 102 CFU/ml in agarose gel electrophoresis. Besides, great sensitivity was also shown in the LFA strip, and no cross reaction existed for other bacteria. Furthermore, Av. paragallinarum in clinical chickens with infectious coryza were perfectly detected by our established LFA strip. Our study is the first to develop the LFA integrated with amplification and sample preparation techniques for better nucleic acid detection of Av. paragallinarum, which holds great potential for rapid, accurate, and on-site determination methods for early diagnosis of Av. paragallinarum to control further spreading.


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