scholarly journals In sacco degradation of eight tropical forages

1995 ◽  
Vol 44 (Suppl. 1) ◽  
pp. 194-194
Author(s):  
BP Widyobroto ◽  
S. Padmowijoto ◽  
R. Utomo ◽  
M. Soejono
Keyword(s):  
1995 ◽  
Vol 44 (Suppl. 1) ◽  
pp. 194-194
Author(s):  
BP Widyobroto ◽  
S. Padmowijoto ◽  
R. Utomo ◽  
M. Soejono
Keyword(s):  

2017 ◽  
Vol 1 (1) ◽  
pp. 43-49
Author(s):  
Suryadi Suryadi ◽  
Darlis Darlis ◽  
Suhessy Syarif ◽  
M. Afdal

Penelitian ini bertujuan untuk mengetahui lama waktu fermentasi dan karakteristik degradasi komponen serat jerami jagung fermentasi secara In sacco. Fermentasi jerami jagung secara padat menggunakan  Trichoderma harzianum sebagai stater. Sebanyak 2,5 gram urea, 2,5 gram molases, 2,5 ml sediaan  Trichoderma harzianum dicampur dengan air menjadi 20 ml yang kemudian disemprotkan pada 1000 gram jerami jagung segar. Selanjutnya jerami jagung dimasukkan ke dalam toples plastik dan diperam sesuai dengan perlakuan yaitu 4, 8, 12 dan 16 hari. Uji karakteristik degradasi jerami jagung fermentasi dilakukan dengan metode In sacco atau nylon bag technique. Sebanyak 6 gram sampel jerami dimasukkan ke dalam kantong nylon dengan ukuran 140, 80 mm diinkubasi ke dalam rumen sapi dengan interval waktu 6, 12, 24, 48 dan 72. Penelitian ini  menggunakan rancangan acak lengkap (RAL) dengan perlakuan 4 lama fermentasi dan ulangan 3 untuk tiap perlakuan. Peubah yang diukur adalah karakteristik degradasi meliputi : Nilai fraksi a, nilai fraksi b dan nilai fraksi c dari NDF, ADF dan Hemiselulosa  Jerami jagung fermentasi. Hasil penelitian ini menunjukkan bahwa lama fermentasi berpengaruh nyata terhadap nilai fraksi (a) dan nilai fraksi (b) dari NDF, nilai fraksi (c) dari ADF dan Hemiselulosa, tetapi tidak berpengaruh nyata pada fraksi (a) dan fraksi (b) dari ADF,  fraksi (a) dan fraksi (b) dari hemiselulosa, fraksi (c) dari NDF jerami jagung fermentasi. Kesimpulan: Fermentasi jerami jagung dengan Trichoderma harzianum dapat meningkatkan nilai fraksi (a) dari NDF, ADF dan laju degradasi NDF ADF dan Hemiselulosa. Lama fermentasi yang terbaik pada jerami jagung fermentasi diperoleh pada perlakuan 16 hari.


1998 ◽  
Vol 22 ◽  
pp. 120-122
Author(s):  
A. S. Chaudhry

The need to develop an in vitro method to simulate ruminal digestion of protein foods has long been recognized. An in vitro method must be more rapid, consistent and convenient than in sacco methods. Purified enzymes have been examined in the past to estimate in vitro degradability of protein foods (Poos-Floyd et al., 1985; Aufrere et al., 1991; Luchini et al., 1996) or their fractions (Chaudhry and Webster, 1994). However, the selection of an appropriate enzyme for a range of foodstuffs remains to be realized. This study examined the potential of two enzymes and a centrifuged rumen fluid (CRF) to estimate proteolysis of food proteins.


1997 ◽  
Vol 64 (1) ◽  
pp. 71-75 ◽  
Author(s):  
M. Blümmel ◽  
P. Bullerdieck

AbstractThe need to complement in vitro gas production measurements with residue determination is demonstrated by the recalculation and reassessment of published data on in vitro gas production, in sacco degradabilities and voluntary dry matter intake (DMI). The in sacco degradability — gas volume ratio was determined at 24 and 48 h of incubation, termed partitioning factor (PF) and combined with rate and extent parameters of in sacco degradability and in vitro gas production to predict DMI. In vitro gas production and in sacco degradability characteristics (a + b) and c as described by the equation y = a + b(1−ect) explained 0·373 and 0·668 respectively of the variation in DMI of 19 legume and grass hays. The complementation of gas production parameters by the PF24 increased the R2 value to 0·744 with PF24 accounting for 0·407 of the variation in DMI, the rate of gas production (c) for 0·218 and the extent of gas production (a + b) for 0·119 of the variation in DMI. As a single parameter, PF48 showed the highest correlation (R2 = 0·597) with DMI but the combination of PF4S with rate and extent of in sacco or in vitro gas production measurements did not improve the correlation further, probably due to an intercorrelation between rates of fermentation and PF4S. Hays which were degraded at faster rates had higher PF values indicating proportionally higher microbial yield and lower short-chain fatty acid production per unit substrate degraded. Generally, hays with high in sacco degradabilities but proportionally low gas production i.e. hays with high PF values showed higher DMI.


1994 ◽  
Vol 39 (1) ◽  
pp. 49-52 ◽  
Author(s):  
T. Stensig ◽  
M.R. Weisbjerg ◽  
J. Madsen ◽  
T. Hvelplund

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